A comparison of in vitro erosion-like mineral loss between continuous and intermittent acidic exposure with and without human saliva

Creanor, S.L.; Creanor, Siobhan; Al-Harthy, Nutayla

doi:10.1016/j.archoralbio.2010.12.014

Cited by 9 publications

(8 citation statements)

References 34 publications

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“…However, the results of pure lactic acid mixed with saliva or water suggest that severe acidic attacks may overwhelm this protective function. This is in agreement with the findings of Creanor et al [2011], who reported that the defensive role of saliva decreases with prolonged exposure to acid.…”

Section: Discussionsupporting

confidence: 83%

Influence of Human Saliva on the Development of Artificial Erosions

2013

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It was hypothesized that saliva from patients with erosion exhibits lower protective efficacy compared to saliva from patients without erosion, based on in vitro enamel softening studies. A total of 645 enamel specimens were distributed among seven experimental groups. Saliva was gathered from each of 10 volunteers without clinical signs of dental erosion and from 10 patients exhibiting severe erosive defects. Aliquots of 50 ml of saliva from each patient were mixed with sour drops or citric acid, respectively. Pooled saliva, sour drops and citric acid mixed with water served as controls. The enamel specimens were soaked in the respective mixture for 5 min and were subsequently incubated in pure saliva for 2 min. This cycle was repeated three times, then the specimens were kept in 100 ml of saliva for 8 h. Surface microhardness was evaluated at the beginning of the experiment and after each cycle. During the experiments, microhardness decreased significantly in all groups except for the pure saliva group. For sour drops and citric acid mixed with saliva from patients without erosion, the final microhardness was higher compared to the mixture of the two erosive compounds with saliva from patients with erosion. The storage of saliva for 8 h resulted in a certain amount of rehardening, with the highest level of rehardening being observed in the group that was least demineralized (sour drops plus saliva from patients without erosion). It is concluded that salivary components play a crucial role in the development of dental erosion.

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Section: Discussionsupporting

confidence: 83%

Influence of Human Saliva on the Development of Artificial Erosions

2013

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“…In practical terms, this is also important because acidic oral challenges (lactic acid from bacteria generated biofilms, citric acid from foods, etc.) are generally accepted as the primary cause of tooth mineral loss in humans [39]. …”

Section: Introductionmentioning

confidence: 99%

Structural and dynamical studies of acid-mediated conversion in amorphous-calcium-phosphate based dental composites

et al. 2014

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Objective To investigate the complex structural and dynamical conversion process of the amorphous-calcium-phosphate (ACP) -to-apatite transition in ACP based dental composite materials. Methods Composite disks were prepared using zirconia hybridized ACP fillers (0.4 mass fraction) and photo-activated Bis-GMA/TEGDMA resin (0.6 mass fraction). We performed an investigation of the solution-mediated ACP-to-apatite conversion mechanism in controlled acidic aqueous environment with in situ ultra-small angle X-ray scattering based coherent X-ray photon correlation spectroscopy and ex situ X-ray diffraction, as well as other complementary techniques. Results We established that the ACP-to-apatite conversion in ACP composites is a two-step process, owing to the sensitivity to local structural changes provided by coherent X-rays. Initially, ACP undergoes a local microstructural rearrangement without losing its amorphous character. We established the catalytic role of the acid and found the time scale of this rearrangement strongly depends on the pH of the solution, which agrees with previous findings about ACP without the polymer matrix being present. In the second step, ACP is converted to an apatitic form with the crystallinity of the formed crystallites being poor. Separately, we also confirmed that in the regular Zr-modified ACP the rate of ACP conversion to hydroxyapatite is slowed significantly compared to unmodified ACP, which is beneficial for targeted slow release of functional calcium and phosphate ions from dental composite materials. Significance For the first time, we were able to follow the complete solution-mediated transition process from ACP to apatite in this class of dental composites in a controlled aqueous environment. A two-step process, suggested previously, was conclusively identified.

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“…As lesões de erosão foram mais profundas na exposição intermitente e alternada com água deionizada, provavelmente porque 1 minuto não foi tempo suficiente para a fixação da película na superfície dentária para que esta pudesse exercer seu efeito tampão, ajudando na remineralização do dente (Creanor et al, 2011).…”

Section: Estrutura Dentalunclassified

“…A TMR é um método amplamente utilizado para estudos de cárie, necessitando de uma superfície paralela, cortes finos (50-200 μm de espessura), é destrutivo, mas fornece informação da distribuição e da profundidade da lesão. Assim, o método TRM vem sendo modificado para os estudos de erosão e é possível quantificar a extensão da perda ou ganho mineral e a profundidade da erosão em esmalte ou dentina, "in vitro" ou "in situ" (Ablal et al, 2009;Amaechi et al, 1999;Rees, 2004;Creanor et al, 2011;Elton et al, 2009;Pretty et al, 2004;Schlueter et al, 2011).…”

Section: Métodos Quantitativosunclassified

“…Um fator que poderia interferir no resultado desta pesquisa seria a diferença na preparação dos corpos de prova, pois para o método do QLF TM o esmalte não precisa ser planificado (Andrade, 2009;Elton et al, 2009;Pretty et al, 2004;Shimaoka, 2010), embora exista esta possibilidade nos estudos "in vitro" (Ablal et al, 2009;Nakata et al, 2009) Outros fatores que influenciam no processo de remineralização do esmalte são: o tipo de apatita, o tipo e idade do dente (Amaechi et al, 1999;Garone Filho;Silva, 2008;Turssi et al, 2010;White et al, 2010); as características do ácido, como o pH (Venasakulchai et al, 2010), a acidez titulável, as propriedades quelantes , a temperatura, o tempo e a freqüência de exposição (Amaechi et al, 1999) A saliva artificial (G8) foi utilizada como controle negativo em vez de água deionizada, para simular de maneira mais adequada a situação clínica na qual o esmalte é submetido aos desafios erosivos e cabe à saliva a função de proteger o dente através da barreira fornecida pela película adquirida de saliva, pela sua capacidade de remover, diluir e neutralizar os ácidos, além de fornecer íons de cálcio, fosfato e flúor para atuarem no processo de remineralização do esmalte Cheaib;Creanor et al, 2011;Hara et al, 2008). A saliva artificial, apesar de não possuir as proteínas da saliva humana, que fornecem um reservatório de cálcio e fosfato e atuam nas trocas iônicas entre a superfície do dente, a película de saliva e o meio oral, ela é supersaturada em cálcio e fosfato e tem capacidade para repor no esmalte os minerais perdidos (Amaechi et al, 1999;McKnight-Hanes;Whitford, 1992;Rehder Neto et al, 2009;Turssi et al, 2011), mas apesar da existência destes minerais na saliva artificial, nesta pesquisa ela não foi capaz de estabilizar o processo de erosão.…”

Section: Quantificação Da Fluorescência Induzida Por Luz (Qlf™)unclassified

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Efeito de materiais contendo compostos bioativos na remineralização de lesões de erosão no esmalte bovino: estudo in vitro

Pedrosa¹

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A comparison of in vitro erosion-like mineral loss between continuous and intermittent acidic exposure with and without human saliva

Cited by 9 publications

References 34 publications

Influence of Human Saliva on the Development of Artificial Erosions

Influence of Human Saliva on the Development of Artificial Erosions

Structural and dynamical studies of acid-mediated conversion in amorphous-calcium-phosphate based dental composites

Efeito de materiais contendo compostos bioativos na remineralização de lesões de erosão no esmalte bovino: estudo in vitro

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