A comparison of immediate-type respiratory reactions to immunologic and pharmacologic agents in rhesus monkeys

Examination of the role of reaginic antibodies in experimental canine asthma indicates that the immunologic species involved in the airway response to inhaled antigens in allergic dogs is in the 0.030 M DEAE-cellulose fraction, in cuts 19–21 by zone electrophoresis and in the ascending limb of the second peak by Sephadex G-200 chromatography. Reaginic activity is eliminated after heating to 56 °C for 4 h and after treatment with 2-mercaptoethanol. Reaginic activity can be transferred passively to the skin and airway of hyposensitive recipients from hypersensitive allergic dogs. Furthermore, radiolabeled antigens specifically bind to the 0.030 m DEAE-cellulose fraction which primarily contains reaginic activity. We have developed rabbit and guinea pig anti-canine reaginic sera which absorb the reaginic activity. Furthermore, we have demonstrated that rabbit antiserum can produce a reversed Prausnitz-Küstner response in both skin and airways. Because the response is completely abolished by atropine sulfate, we suggest that canine reaginic antibody produces airway constriction in the allergic dog by a vagally-mediated reflex mechanism in addition to a local effect caused by mediator release.

Section: Discussionmentioning

confidence: 99%

Immunologic and Physiologic Characterization of the Role of Reaginic Antibodies in Experimental Asthma in Dogs

Kessler

Frick

Gold³

1974

“…The experimental system of aerosol challenge has previously been described [7,11]. Briefly, aero sol challenges were conducted using a Mark VII Bird respirator with an in-line nebulizer using constant settings on the respiratory apparatus so that the amount of nebulized material delivered through the endotracheal tube to the airways with each inhalation was constant in each experiment [10].…”

Section: Aerosol Challenge and Determination Of Pulmonary Function Pamentioning

confidence: 99%

Differential Airway Reactivity to Carbachol and Physostigmine Sulfate in Rhesus Monkeys with and without Reagin-Mediated Respiratory Responses

Miller¹,

Patterson²

1977

Self Cite

Bronchial airway reactivity to carbachol (CAR) and physostigmine (PHY) was individually measured in three rhesus monkeys with and three rhesus monkeys without reagin-mediated respiratory responses (RR). The three monkeys with reagin-mediated RR demonstrated bronchial hyperreactivity to CAR (p <0.25) but not PHY when compared with the three control monkeys. No correlation between airway reactivity to CAR and PHY was noted in all six monkeys. We conclude that monkeys with reagin-mediated RR possess airway hyperreactivity to the exogenously administered acetylcholine analogue CAR, but not to the endogenous acetylcholine which is released in vivo by PHY.

“…A variety of animal models have been developed to at tempt to examine these problems. Animal models advocated have included guinea pigs [1], dogs [2] and monkeys [7], The rat has been used in the anaphylactic bronchial constriction model [4] in which the intrave nous injection of antigen in the sensitized pitched rat produces bronchial constriction. We have previously demonstrated [3] that appropriately sensitized conscious rats re spond to aerosol challenge delivered via the airway by characteristic alterations in re spiration.…”

Section: Introductionmentioning

confidence: 99%

Site of Respiratory Reaction in Allergic Rats Challenged via the Airways

Carswell¹,

Oliver²

1978

Rats systemically sensitized to DNP-ovalbumin show a characteristic respiratory response to antigen challenge delivered via the airway. The slowing of expiratory air flow which occurs on aerosol challenge still occurs when the antigen is injected via a tracheostomy but not when it is delivered solely to the upper airways. Disodium cromoglycate administered via the tracheostomy 1 min before intratracheal antigen challenge reduces the respiratory response.