A Comparison of Cryptosporidium Subgenotypes from Several Geographic Regions

Molecular Insights for Giardia, Cryptosporidium, and Soil-Transmitted Helminths from a Facility-Based Surveillance System in Guatemala

“…A previous report from Guatemala described only the anthroponotic C. hominis from four people. 20 Analysis of parasite infections by age showed that cryptosporidiosis was detected in young children, a finding that is in concordance with previous reports, 21 whereas infections with the other three parasites were not. This difference in age susceptibility to infections is important, because BZ-based MDA campaigns are focused on school age children.…”

Identification of a High Diversity of Cryptosporidium Species Genotypes and Subtypes in a Pediatric Population in Nigeria

“…Species data have been collected in only five countries; Kenya, Malawi, Uganda, Equatorial Guinea, and South Africa and include C. hominis , C. parvum , C. canis , C. felis , C. meleagridis , and C. muris . 5,16,17,22,23,39 However, only in Kenya have all six species been reported. 16 Cryptosporidium hominis was the dominant species identified in our study.…”

“…20,21 Although cryptosporidiosis is prevalent in developing countries, genetic characterization of species is lacking, especially in Africa, where only four subgenotyping studies have been conducted in Uganda, Malawi, Kenya, and South Africa. 7,15,22,23 Cryptosporidium meleagridis , although not found as commonly as C. hominis and C. parvum , is the third most common infection in humans. 4,5,14,18,24,25 Two subtypes of C. meleagridis have been identified at the small subunit (SSU) ribosomal RNA (rRNA) gene locus, and six 6 subtypes have been identified at the GP60 gene locus, 26 which indicate possible heterogeneity in host range, and therefore routes of transmission, for C. meleagridis .…”

Subgenotype Analysis of Cryptosporidium Isolates from Humans, Cattle, and Zoo Ruminants in Portugal

“…Sequence alignment revealed extensive differences in the nucleotide sequence through the entire length of the fragment and divided all C. parvum and C. hominis isolates into seven alleles (Fig. 1) (8,15,18,19). Within C. parvum alleles, allele IIa contained all the isolates from the zoo's wild ruminants, 29 of the 32 isolates from calves, and 4 human isolates.…”

“…A fragment of the GP60 gene (800 to 850 bp) was amplified by a nested PCR, with the primers AL3531 and AL3535 (5Ј-GGA AGG AAC GAT GTA TCT-3Ј) in the primary PCR and AL3532 and AL3534 in the secondary PCR (15). PCRs were performed as previously described (5).…”