A comparison of genetic variations among native andsome local chicken populations in Turkey

Abstract

Genetic variations among native and local chicken populations in six different locations across Turkey were established using 15 ISSR primers, and produced 87 bright and reproducible bands. According to pairwise genetic differentiation among the populations (G ST ), the highest genetic differentiation was determined between the Samsun and Yozgat population and the lowest was observed between the Dulkadirli and Budak populations. Shannon's index was calculated to be 0.239. The gene flow (Nm) among the populations was estimated to be 3.489. Analysis of Molecular Variance (AMOVA) identified 13% of the total genetic variation between the populations and the rest of the differences were 87% within the populations. Cluster analysis revealed two main branches, one leading to the domestic chicken population collected from Samsun in the Black Sea region of Turkey, the other branch clustered into two branches; one branch consisting of the Denizli Native Chicken population and the other one of domestic chicken populations sampled from Central Anatolia populations. The study is important to clarify the indigenous chicken genetic resources in Turkey. The results could be used for future breeding research conducted by either the public or private sectors.

SSR and ISSR markers in assessing genetic diversity in Gallus gallus domesticus: a quantitative analysis of scientific production

“…al.., 2007 the eukaryote genome and highly polymorphic being adequate to characterize and estimate genetic distance between population, allowing the comparison between races and the comprehension of the nature of avian biodiversity (ABOU-EMERA et al, 2017). The ISSR marker is of simple use, besides being cheaper than SSR, don't need previous knowledge of the genome and might be more frequently used in the future on research of chicken or birds in general due to these advantages (TUNCA & TASKIN, 2016). However, ISSR is characterized by being a dominant marker and the presence of amplified fragment might mean a dominant homozygous or heterozygous, while its absence represents a recessive homozygous, because is lowest informative than SSR; uhowever, been useful for characterization of genetic structure, mainly in preliminary studies (COSTA et al 2015).…”

“…The table 2 also demonstrated that researches involving molecular markers and chicken aimed to assess the level of genetic variability in populations from different places, highlighting any possible distinction between chickens under different environments. These studies are essentials when the aim is to maintain genetic variability to be useful in future crosses (TUNCA & TASKIN, 2016).…”