A Comparison of Five Treatment Protocols for Contaminated Bone Grafts in Reference to Sterility and Cell Viability

Bauer, Jennifer M.; Liu, Raymond W.; Kean, Thomas J.; Dennis, James E.; Petersilge, William J.; Gilmore, Allison

doi:10.2106/jbjs.j.00418

Cited by 33 publications

(32 citation statements)

References 19 publications

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“…Furthermore, the increased exposure time allowed drying of the PI solution, which has been shown to increase the effectiveness at surface decontamination. 51 This leads us to believe that 30 seconds is likely a sufficient conjunctival exposure time to cause a significant reduction in CFUs. It is possible that the BSS irrigation used in our study does not allow actual representation of the sterilizing potential of PI because many practitioners do not wash the inferior fornix until after the intraocular injection is complete.…”

Section: Resultsmentioning

confidence: 99%

Povidone–iodine Contact Time and Lid Speculum Use During Intravitreal Injection

Friedman

Mason

Emond

et al. 2013

Retina

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Section: Resultsmentioning

confidence: 99%

Povidone–iodine Contact Time and Lid Speculum Use During Intravitreal Injection

Friedman

Mason

Emond

et al. 2013

Retina

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“…Some disinfection methods, for example, gamma irradiation, chemical disinfection, microwave, or autoclaving have been shown to more efficiently inactivate viruses and bacteria than other treatments [19–23, 33–35], for example, using high hydrostatic pressure or thermal disinfection at 82.5°C with a limited effect on the viability of pathogens while preserving the biomechanical integrity [13, 18]. Thermal disinfection at 82.5°C for at least 15 min was showed to be very efficient to eliminate virus, vegetative bacteria, fungi, and fungal spores, but heat-resistant spores of B. subtilis and C. sporogenes were reduced only by one to two orders of magnitude [13].…”

Section: Discussionmentioning

confidence: 99%

Comparative Biomechanical and Microstructural Analysis of Native versus Peracetic Acid-Ethanol Treated Cancellous Bone Graft

Rauh

Despang

Baas

et al. 2014

BioMed Research International

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Bone transplantation is frequently used for the treatment of large osseous defects. The availability of autologous bone grafts as the current biological gold standard is limited and there is a risk of donor site morbidity. Allogenic bone grafts are an appealing alternative, but disinfection should be considered to reduce transmission of infection disorders. Peracetic acid-ethanol (PE) treatment has been proven reliable and effective for disinfection of human bone allografts. The purpose of this study was to evaluate the effects of PE treatment on the biomechanical properties and microstructure of cancellous bone grafts (CBG). Forty-eight human CBG cylinders were either treated by PE or frozen at −20°C and subjected to compression testing and histological and scanning electron microscopy (SEM) analysis. The levels of compressive strength, stiffness (Young's modulus), and fracture energy were significantly decreased upon PE treatment by 54%, 59%, and 36%, respectively. Furthermore, PE-treated CBG demonstrated a 42% increase in ultimate strain. SEM revealed a modified microstructure of CBG with an exposed collagen fiber network after PE treatment. We conclude that the observed reduced compressive strength and reduced stiffness may be beneficial during tissue remodeling thereby explaining the excellent clinical performance of PE-treated CBG.

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“…Investigations of decontamination regimes conclude that washing with diluted iodine solutions is recommended. 46,47 It can be seen by these entirely contradictory experimental results across a range of disparate studies that evidence may be found for both cytotoxicity and tolerance of fibroblasts, chondrocytes, keratinocytes, and bone-forming cells to iodine solutions. No consensus exists apart from the trend of increased tolerance for iodine solutions when applied at low concentrations, which as previously established, coincides with the maximal antimicrobial efficacy of the solution.…”

Section: Use In Periarticular Trauma and Concern Over Chondrotoxicitymentioning

confidence: 99%

Limb Wounding and Antisepsis

Eardley

Watts

Clasper

2012

The International Journal of Lower Extremity Wounds

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Extremity injury and contamination as consequence are features of high-energy wounding. A leading cause of disability and the commonest cause of late complications, prevention of wound infection determines the ultimate outcome in these populations. Multiple variables influence the development of infection, one of which is the dressing used on the wound. Antiseptic-soaked gauze dressings feature in the early management of limb trauma despite a lack of evidence to support this. Iodine and chlorhexidine are ubiquitous in other aspects of health care however, and a plethora of studies detail their role in skin antisepsis, the recommendations from which are often anecdotally applied to acute wounding. To contextualize the role for antiseptic dressing use in acute, significant limb injury this review explores the evidence for the use of chlorhexidine and iodine in skin antisepsis. The paucity of experimental data available for antiseptic use in early wound management and the need for further research to address this evidence void is highlighted.

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A Comparison of Five Treatment Protocols for Contaminated Bone Grafts in Reference to Sterility and Cell Viability

Abstract: Of the easily accessible protocols studied, mechanical agitation and serial washes of bone graft in povidone-iodine that is allowed to dry offers the best balance between complete sterilization of contaminated bone and maintenance of tissue viability.

Cited by 33 publications

References 19 publications

Povidone–iodine Contact Time and Lid Speculum Use During Intravitreal Injection

Povidone–iodine Contact Time and Lid Speculum Use During Intravitreal Injection

Comparative Biomechanical and Microstructural Analysis of Native versus Peracetic Acid-Ethanol Treated Cancellous Bone Graft

Limb Wounding and Antisepsis

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