Comparative Biomechanical and Microstructural Analysis of Native versus Peracetic Acid-Ethanol Treated Cancellous Bone Graft

Abstract

Bone transplantation is frequently used for the treatment of large osseous defects. The availability of autologous bone grafts as the current biological gold standard is limited and there is a risk of donor site morbidity. Allogenic bone grafts are an appealing alternative, but disinfection should be considered to reduce transmission of infection disorders. Peracetic acid-ethanol (PE) treatment has been proven reliable and effective for disinfection of human bone allografts. The purpose of this study was to evaluate the effects of PE treatment on the biomechanical properties and microstructure of cancellous bone grafts (CBG). Forty-eight human CBG cylinders were either treated by PE or frozen at −20°C and subjected to compression testing and histological and scanning electron microscopy (SEM) analysis. The levels of compressive strength, stiffness (Young's modulus), and fracture energy were significantly decreased upon PE treatment by 54%, 59%, and 36%, respectively. Furthermore, PE-treated CBG demonstrated a 42% increase in ultimate strain. SEM revealed a modified microstructure of CBG with an exposed collagen fiber network after PE treatment. We conclude that the observed reduced compressive strength and reduced stiffness may be beneficial during tissue remodeling thereby explaining the excellent clinical performance of PE-treated CBG.

Characterization of Bone Marrow Mononuclear Cells on Biomaterials for Bone Tissue EngineeringIn Vitro

“…It is safe to assume that the impairment of adhesion and function is not limited to osteoblasts; other cell types may also be affected. The DBM used in this study received a peracetic acid treatment which is a validated and “a reliable sterilization method” [ 38 , 39 ] but may alter mechanical properties of the DBM [ 40 ]. Biocompatibility, however, appears not to be significantly impaired by this sterilization procedure: Endres et al cultured bone marrow derived cells on differentially pretreated allogeneic bone transplants and assessed cell vitality and the percentages of immune cell subpopulations four weeks after transplantation.…”

Comparison of Antimicrobial Activity between Chemical Disinfectants on Contaminated Orthodontic Pliers

“…New disinfecting products have been recently introduced to the market, with good results in medicine practice. 7,15 Therefore, it is important to evaluate them in dentistry also.…”

Vreden Russian Research Institute of Traumatology and Orthopedics

“…Так, например, j. Rauh c соавторами используют способ, при котором костные имплантаты инкубируют в смеси хло-роформа (99,4%) и метанола (99,8%) в соотно-шении 2/1 в течении 2 ч, затем восьмикратно (по 15 мин.) промывают в ультразвуковой мойке в растворе метанола, затем костные имплантаты дважды промывают от химических веществ де-ионизированой водой [31].…”

“…j. Rauh с соавторами в своем исследовании продемон-стрировали высокие стерилизующие свойства, сохранение механической прочности костных имплантатов, а также отметили, что этот метод позволяет создавать материал, пригодный для заселения культуры клеток [31]. Однако в лите-ратуре описаны 9 случаев вторичного инфици-рования после имплантации материалов, стери-лизованных этим способом [37].…”