A comparison of cultural characteristics and infectivity of Frankia isolates from root nodules of Casuarina species

Zhang, Zhongze; Lopez, Mary F.; Torrey, John G.

doi:10.1007/978-94-009-6158-6_8

Cited by 74 publications

(60 citation statements)

References 7 publications

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“…CcI3 was isolated from Casuarina cunninghamiana plants growing in a greenhouse at Harvard Forest in Petersham, MA (Zhang et al 1984) on soils coming from its original provenance. ACN was isolated initially from Alnus viridis subsp.…”

Section: Methods Strainsmentioning

confidence: 99%

Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography

Normand¹,

Lapierre²,

Tisa³

et al. 2006

Genome Res.

347

298

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Soil bacteria that also form mutualistic symbioses in plants encounter two major levels of selection. One occurs during adaptation to and survival in soil, and the other occurs in concert with host plant speciation and adaptation. Actinobacteria from the genus Frankia are facultative symbionts that form N 2 -fixing root nodules on diverse and globally distributed angiosperms in the "actinorhizal" symbioses. Three closely related clades of Frankia sp. strains are recognized; members of each clade infect a subset of plants from among eight angiosperm families. We sequenced the genomes from three strains; their sizes varied from 5.43 Mbp for a narrow host range strain (Frankia sp. strain HFPCcI3) to 7.50 Mbp for a medium host range strain (Frankia alni strain ACN14a) to 9.04 Mbp for a broad host range strain (Frankia sp. strain EAN1pec.) This size divergence is the largest yet reported for such closely related soil bacteria (97.8%-98

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Section: Methods Strainsmentioning

confidence: 99%

Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography

Normand¹,

Lapierre²,

Tisa³

et al. 2006

Genome Res.

347

298

View full text Add to dashboard Cite

show abstract

“…The Frankia CcI3 genome is completely sequenced (22), and it represents a narrow-host-range symbiont. The relationship between the plant host and symbiont, including the nodulation process, is well studied (17,18,27,35,41). The purpose of this study was to develop a system to identify chemical signaling between the microbe and its host plant.…”

mentioning

confidence: 99%

Casuarina Root Exudates Alter the Physiology, Surface Properties, and Plant Infectivity of Frankia sp. Strain CcI3

Beauchemin

Furnholm

Lavenus

et al. 2012

Appl Environ Microbiol

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The actinomycete genus Frankia forms nitrogen-fixing symbioses with 8 different families of actinorhizal plants, representing more than 200 different species. Very little is known about the initial molecular interactions between Frankia and host plants in the rhizosphere. Root exudates are important in Rhizobium-legume symbiosis, especially for initiating Nod factor synthesis. We measured differences in Frankia physiology after exposure to host aqueous root exudates to assess their effects on actinorhizal symbioses. Casuarina cunninghamiana root exudates were collected from plants under nitrogen-sufficient and -deficient conditions and tested on Frankia sp. strain CcI3. Root exudates increased the growth yield of Frankia in the presence of a carbon source, but Frankia was unable to use the root exudates as a sole carbon or energy source. Exposure to root exudates caused hyphal "curling" in Frankia cells, suggesting a chemotrophic response or surface property change. Exposure to root exudates altered Congo red dye binding, which indicated changes in the bacterial surface properties at the fatty acid level. Fourier transform infrared spectroscopy (FTIR) confirmed fatty acid changes and revealed further carbohydrate changes. Frankia cells preexposed to C. cunninghamiana root exudates for 6 days formed nodules on the host plant significantly earlier than control cells. These data support the hypothesis of early chemical signaling between actinorhizal host plants and Frankia in the rhizosphere.A ctinorhizal symbioses are mutualistic interactions that occur between actinorhizal plants and the actinomycete genus Frankia (27,38). Frankia exists either in a free-living state in the soil or in symbiosis with actinorhizal plants (2, 4, 31). Actinorhizal plants consist of 8 different plant families, including over 200 different species of woody dicotyledonous trees and shrubs (31,38,42). The globally distributed actinorhizal plants are found on every continent except Antarctica and are able to grow in a diverse set of natural habitats, including arid lands, plains, tundra, and temperate forests. Actinorhizal plants are pioneer plant species that are able to grow in extremely nutrient-poor soil conditions and, with the aid of Frankia, are able to reclaim surrounding soil in disrupted environments. Actinorhizal plants are important in agroforestry, in soil reclamation, and as a fuel source.While actinorhizal symbiosis has been well studied at a morphological level since the 1970s, very little is known about the molecular interactions that occur between the plant hosts and Frankia during the establishment of the association. The establishment of the symbiosis encompasses the infection and nodulation processes (23,27,38). In general, the bacterium needs to recognize a host plant and the host needs to identify the bacterium as a friend, not a foe. The bacteria enter the plant and establish the association after several steps. The infection ultimately leads to the formation of the mature nodule.There is a paucity of information o...

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“…Cultures of Frankia strain CcI3, originally isolated from root nodules of Casuarina cunninghamiana (27), were grown on a defined nitrogen-free medium (3) with propionate as the carbon source. Growth was in 1-liter flasks without shaking, for a period of 1 to 3 months.…”

mentioning

confidence: 99%

Hemoglobin in Frankia , a Nitrogen-Fixing Actinomycete

Tjepkema

Cashon

Beckwith

et al. 2002

Appl Environ Microbiol

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A comparison of cultural characteristics and infectivity of Frankia isolates from root nodules of Casuarina species

Cited by 74 publications

References 7 publications

Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography

Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography

Casuarina Root Exudates Alter the Physiology, Surface Properties, and Plant Infectivity of Frankia sp. Strain CcI3

Hemoglobin in Frankia , a Nitrogen-Fixing Actinomycete

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