A Comparison of Conventional Collagen Sponge and Collagen-Gelatin Sponge in Wound Healing

Abstract

The objective of this study was to compare the effectiveness of the collagen-gelatin sponge (CGS) with that of the collagen sponge (CS) in dermis-like tissue regeneration. CGS, which achieves the sustained release of basic fibroblast growth factor (bFGF), is a promising material in wound healing. In the present study, we evaluated and compared CGSs and conventional CSs. We prepared 8 mm full-thickness skin defects on the backs of rats. Either CGSs or CSs were impregnated with normal saline solution (NSS) or 7 μg/cm2 of bFGF solution and implanted into the defects. At 1 and 2 weeks after implantation, tissue specimens were obtained from the rats of each group (n = 3, total n = 24). The wound area, neoepithelial length, dermis-like tissue area, and the number and area of capillaries were evaluated at 1 and 2 weeks after implantation. There were no significant differences in the CGS without bFGF and CS groups. Significant improvements were observed in the neoepithelial length, the dermis-like tissue area, and the number of newly formed capillaries in the group of rats that received CGSs impregnated with bFGF. The effects on epithelialization, granulation, and vascularization of wound healing demonstrated that, as a scaffold, CGSs are equal or superior to conventional CSs.

Assessment of the characteristics and biocompatibility of gelatin sponge scaffolds prepared by various crosslinking methods

“…Gelatin is a partial hydrolysis product of native collagen and characterized by non-toxicity, non-carcinogenicity, biocompatibility, and biodegradability 1 – 3 ; gelatin is widely used in the pharmaceutical and medical fields, such as in wound dressing materials 4 , 5 , tissue engineering scaffolds 6 – 8 , and drug delivery carriers 9 , 10 . Gelatin can be prepared in a spongy form to be suitable for tissue engineering applications.…”

Polyethylenimine-alginate nanocomposites based bone morphogenetic protein 2 gene-activated matrix for alveolar bone regeneration

“…5,6 A previous study observed new bone formation in the maxillary sinus using only gelatin sponges as the gra material, which may provide adequate bone mass for implant placement. 7 Since gelatin sponges have excellent biocompatibility, degradation into physiological endproducts [8][9][10] and suitable interaction with cells and other macromolecules, [11][12][13][14] they have received increasing attention as scaffolds in the emerging eld of tissue engineering. [15][16][17] Bone morphogenetic proteins (BMPs) are potent growth factors for bone formation and remodeling, due to their powerful osteoinductive ability.…”

A comparison of the wound healing process after the application of three dermal substitutes with or without basic fibroblast growth factor impregnation in diabetic mice

“…Newly formed capillaries stained with visible lumen with erythrocytes on anti-CD31 stained sections were evaluated. The number and area of the stained capillaries in 3 squares of 200 μm × 200 μm at the center and both marginal wounds in each section were counted manually 21 . The sum of the capillary numbers or the area in square microns observed in the three squares in each section was used for evaluation.…”