A comparison of allogeneic and autologous mesenchymal stromal cells and osteoprogenitor cells in augmenting bone formation around massive bone tumor prostheses

Coathup, Melanie; Kalia, Priya; Konan, Sujith; Mirza, Kamran; Blunn, Gordon

doi:10.1002/jbm.a.34536

Cited by 17 publications

(17 citation statements)

References 33 publications

(54 reference statements)

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“…However, a radiolucent line between the extracortical bone and the shaft of the implant in the Noncollar Group clearly was evident in all cases except one. The importance of osteointegration at the shoulder has been recognized and some experimental studies have investigated methods to enhance extracortical bone formation, including the use of bone morphogenetic proteins [22] and stem cells [9,18].…”

Section: Discussionmentioning

confidence: 99%

Hydroxyapatite-coated Collars Reduce Radiolucent Line Progression in Cemented Distal Femoral Bone Tumor Implants

Coathup

Sanghrajka

Aston

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Aseptic loosening of massive bone tumor implants is a major cause of prosthesis failure. Evidence suggests that an osteointegrated hydroxyapatite (HA)-coated collar would reduce the incidence of aseptic loosening around the cemented intramedullary stem in distal femoral bone tumor prostheses. Because these implants often are used in young patients with a tumor, such treatment might extend the longevity of tumor implants. Questions/purposes We asked whether (1) HA-coated collars were more likely to osteointegrate; (2) HA collars were associated with fewer progressive radiolucent lines around the stem-cement interface; and (3) HA-coated collars were associated with less bone loss at the boneshoulder implant junction? Methods Twenty-two patients were pair-matched to one of two groups-either (1) implants with a HA-coated ingrowth collar (HA Collar Group); or (2) implants without an ingrowth collar (Noncollar Group). Age, sex, and length of followup were similar in both groups. HA-coated collars were developed and used at our institution from 1992 to address the high failure rate attributable to aseptic loosening in patients with massive bone tumor implants. Before this, smooth titanium shafts were used routinely adjacent to bone at the transection site. The minimum followup was 2 years (mean, 7 years; range, 2-12 years). Radiographs obtained throughout the followup period were analyzed and osteointegration at the shaft of the implant quantified. Radiolucent line progression around the cemented stem was semi-quantitatively assessed and cortical bone loss at the bone-shoulder implant junction was measured during the followup period.

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Section: Discussionmentioning

confidence: 99%

Hydroxyapatite-coated Collars Reduce Radiolucent Line Progression in Cemented Distal Femoral Bone Tumor Implants

Coathup

Sanghrajka

Aston

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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“…Although most previous studies have used allogeneic MSCs due to their unique properties, some studies have reported that allogeneic MSCs induce memory T‐cell response resulting in cell rejection (Nauta et al , ). An in vivo study reported that no osteointegration was seen in allogeneic MSCs, and that an increased immunological response within soft tissue was observed, compared with autologous MSCs (Coathup et al , ). However, no T‐cell response with inflammatory markers was detected.…”

Section: Discussionmentioning

confidence: 99%

Monitoring immune response after allogeneic transplantation of mesenchymal stem cells for osteochondral repair

Mahmoud

Tanaka

Kamei

et al. 2017

J Tissue Eng Regen Med

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The aim of this study was to investigate the safety of using allogeneic magnetically labelled mesenchymal stem cells (m-MSCs) to ameliorate osteochondral repair, with immune surveillance using a mixed lymphocyte reaction (MLR) assay. Twenty knees of Japanese white rabbits were randomly divided into two groups: the control (autologous) group, where 2 × 10 5 autologous m-MSCs were transplanted into the defect site; the experimental (allogeneic) group, where 2 × 10 5 m-MSCs from Dutch rabbits were transplanted into the defect of Japanese white rabbits. The rabbits were then euthanized after 12 weeks. The repaired tissue was stained with toluidine blue stain in order to produce histological scoring on the Fortier scale. Splenocytes were used to evaluate anti-donor alloreactivity by a MLR assay using the carboxyfluorescein diacetate succimidyl ester (CFSE) labelling technique (CFSE-MLR). In both groups, complete repair of the subchondral bone covered by a layer of chondrogenic tissue was confirmed. Also, there was no histologically significant difference on the Fortier scale. Using the CFSE-MLR assay, CD4+ T-cells showed alloreactivity in each combination, while the stimulation index of CD4+ T-cells was not statistically different between the control and experimental groups. Allogeneic m-MSCs are a safe alternative source to autologous m-MSCs, upholding repair of an osteochondral defect for clinical application using universal donor MSCs through a one-stage surgical procedure.

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“…Kalia et al 126 and Coathup et al 127 sprayed autologous and allogenic MSCs or differentiated MSC-derived osteoblasts in fibrin glue onto hydroxyapatite coated ingrowth collars of massive bone tumor implants and studied the integration of these implants in a sheep model. 126,127 Autologous MSCs and MSC-derived osteoblasts but not allogenic MSCs increased implant integration. Thus transplanted autologous MSCs can be utilized to enhance initial implant integration but clinical studies in humans remain to be conducted.…”

Section: Mscs For Improving Initial Implant Integrationmentioning

confidence: 99%

Mesenchymal stem cells in the aseptic loosening of total joint replacements

Pajarinen

Lin

Nabeshima

et al. 2017

J Biomedical Materials Res

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Peri-prosthetic osteolysis remains as the main long-term complication of total joint replacement surgery. Research over four decades has established implant wear as the main culprit for chronic inflammation in the peri-implant tissues and macrophages as the key cells mediating the host reaction to implant-derived wear particles. Wear debris activated macrophages secrete inflammatory mediators that stimulate bone resorbing osteoclasts; thus bone loss in the peri-implant tissues is increased. However, the balance of bone turnover is not only dictated by osteoclast-mediated bone resorption but also by the formation of new bone by osteoblasts; under physiological conditions these two processes are tightly coupled. Increasing interest has been placed on the effects of wear debris on the cells of the bone-forming lineage. These cells are derived primarily from multipotent mesenchymal stem cells (MSCs) residing in bone marrow and the walls of the microvasculature. Accumulating evidence indicates that wear debris significantly impairs MSC-to-osteoblast differentiation and subsequent bone formation. In this review, we summarize the current understanding of the effects of biomaterial implant wear debris on MSCs. Emerging treatment options to improve initial implant integration and treat developing osteolytic lesions by utilizing or targeting MSCs are also discussed.

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A comparison of allogeneic and autologous mesenchymal stromal cells and osteoprogenitor cells in augmenting bone formation around massive bone tumor prostheses

Cited by 17 publications

References 33 publications

Hydroxyapatite-coated Collars Reduce Radiolucent Line Progression in Cemented Distal Femoral Bone Tumor Implants

Hydroxyapatite-coated Collars Reduce Radiolucent Line Progression in Cemented Distal Femoral Bone Tumor Implants

Monitoring immune response after allogeneic transplantation of mesenchymal stem cells for osteochondral repair

Mesenchymal stem cells in the aseptic loosening of total joint replacements

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