A Comparison Between Shaker and Bioreactor Performance Based on the Kinetic Parameters of Xanthan Gum Production

Faria, Sandra; Vieira, Patrícia Angélica; Resende, Miriam Maria de; França, Francisca Pessôa de; Cardoso, Vicelma Luiz

doi:10.1007/s12010-008-8485-8

Cited by 20 publications

(17 citation statements)

References 25 publications

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“…The xanthan market is about US $270 million and it is expected to reach US $400 million with a production of 80,000 tons/year in 2015 [2]. In the industrial production is used 2-4% of carbon source as sucrose or glucose, and 0.05-0.1% of nitrogen source as yeast extract, peptone, ammonium nitrate or urea [3].…”

Section: Introductionmentioning

confidence: 99%

New Culture Medium to Xanthan Production by Xanthomonas campestris pv. campestris

et al. 2011

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Xanthan is a important biopolymer for commercial purpose and it is produced in two stages by Xanthomonas campestris. In the first one, the bacterium is cultivated in the complex medium enriched in nitrogen and the biomass produced is used as inoculum for the next stage in which the gum is produced in another medium. In this work a new medium for the first stage is proposed in place of currently used YM medium. Different formulated growth media were studied and the correspondent biomass produced was analysed as inoculum for the second stage. The inoculum and gum were produced by batch process in shaker at 27°C in pH 6.0 and at 30°C in pH 7.0, respectively. The gum was precipitated with ethanol (3:1 v/v). The dryed biomass and xathan gum produced were determined by drying in oven at 105 and 40°C, respectively. The viscosity of the fermentation broth and 1% gum solution in water were determined in Brookfield viscometer. The formulated medium presented the increase in gum production (30%), broth (136%) and 1% gum solution viscosity (60%) compared to YM, besides the inferior cost. The results showed the importance of the quality of the inoculum from the first stage of the culture which influenced on the gum viscosity in the second stage.

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Section: Introductionmentioning

confidence: 99%

New Culture Medium to Xanthan Production by Xanthomonas campestris pv. campestris

et al. 2011

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“…The supernatant was filtered and treated with a saturated solution of KCl, and the polymer was recovered by precipitation with ethanol. Finally, the product was dried under vacuum system at 30 ± 1 °C [10] .…”

Section: Recovery and Purification Of Biopolymermentioning

confidence: 99%

“…The precipitate (cells) was washed three times in distilled water for complete removal of medium components and metabolites [10] .…”

Section: Determination Of Biomassmentioning

confidence: 99%

“…The rheology of gum was obtained by preparing a polymer solution of 0.5%, 0.75% and 1% under magnetic stirring for approximately 10 h. The gum used to prepare this solution was dried at room temperature, and then crushed and hydrated [10,12] .The rheology was measured using a Brookfield rheometer RVDVIII coupled with a water bath mark Brookfield, model TC-502P, using the small sample adapter, spindle 18. Following the template Ostwald Waele or power-law, data on shear stress, measured from shear rates, were used.…”

Section: Evaluation Of Rheological Behavior Of the Biopolymermentioning

confidence: 99%

“…Faria et al [10] also studied the effect of the addition of yeast extract on the production of xanthana gum by Xanthomonas campestris pv. using broth cane sugar as a carbon source, and found that the optimum condition of the yeast extract concentration was 1.8 g.L -1…”

Section: Experiments Employing Stirred Conical Flasksmentioning

confidence: 99%

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Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate

et al. 2016

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SbstractThis study evaluated extracellular production of biopolymer using fungus Mucor racemosus Fresenius and glycerol as a carbon source. Initially employing conical flasks of 500 mL containing 100 mL of cultive medium with 0.18 ± 0.03 g.L -1 of microorganisms, the results showed that the best conditions of the variables studied were: initial concentration of glycerol 50 g.L gum formed and 75% glycerol consumption. These conditions produced a biopolymer with the molecular weight and total sugar content of 4.607×10 6 g.mol -1 (Da) and 89.5%, respectively.

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Modeling and steady state simulation: production of xanthan gum from sugarcane broth

Vignesh

Arumugam

Venkatachalam

2014

Bioprocess Biosyst Eng

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The work is focused on developing a mathematical model for continuous process of xanthan gum production. The main objective of the study is to simulate the model, observe the behavior of substrate consumption, biomass and product formation with respect to dilution rate and determine the optimum dilution rate for which the reactor is to be designed. Systems with and without recycling of cells are considered and the optimum dilution rate is found. For the kinetic parameters used, the optimum dilution rate for the system with no recycling is 0.205 and 0.35 h(-1) for the system that includes recycling of cells.

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A Comparison Between Shaker and Bioreactor Performance Based on the Kinetic Parameters of Xanthan Gum Production

Cited by 20 publications

References 25 publications

New Culture Medium to Xanthan Production by Xanthomonas campestris pv. campestris

New Culture Medium to Xanthan Production by Xanthomonas campestris pv. campestris

Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate

Modeling and steady state simulation: production of xanthan gum from sugarcane broth

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