A comparative toxicity study between small and large size zinc oxide nanoparticles in tilapia (Oreochromis niloticus): Organ pathologies, osmoregulatory responses and immunological parameters

Kaya, Hasan; Aydın, Fatih; Gürkan, Mert; Yılmaz, Sevdan; Ateş, Mehmet; Demir, Veysel; Arslan, Zikri

doi:10.1016/j.chemosphere.2015.09.024

Cited by 103 publications

(39 citation statements)

References 59 publications

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“…Though differences in LA and MPO activities could be associated with morphological differences, overall these results demonstrate immunotoxicity of Fe 2 O 3 NPs even at environmentally feasible concentrations. Similar adverse effects were observed from ZnO NPs on O. niloticus ( Kaya et al, 2016 ) . While RBA levels were increased by small ZnO NPs, large ZnO NPs showed suppressive effects.…”

Section: Discussionsupporting

confidence: 72%

Chronic exposure of tilapia (Oreochromis niloticus) to iron oxide nanoparticles: Effects of particle morphology on accumulation, elimination, hematology and immune responses

et al. 2016

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Effects of chronic exposure to alpha and gamma iron oxide nanoparticles (α-Fe2O3 and γ-Fe2O3 NPs) were investigated through exposure of tilapia (Oreochromis niloticus) to 0.1, 0.5 and 1.0 mg/L (9.2×10−4, 4.6×10−3 and 9.2×10−3 mM) aqueous suspensions for 60 days. Fish were then transferred to NP-free freshwater and allowed to eliminate ingested NPs for 30 days. The organs, including gills, liver, kidney, intestine, brain, spleen, and muscle tissue of the fish were analyzed to determine the accumulation, physiological distribution and elimination of the Fe2O3 NPs. Largest accumulation occurred in spleen followed by intestine, kidney, liver, gills, brain and muscle tissue. Fish exposed to γ-Fe2O3 NPs possessed significantly higher Fe in all organs. Accumulation in spleen was fast and independent of NP concentration reaching to maximum levels by the end of the first sampling period (30th day). Dissolved Fe levels in water were very negligible ranging at 4–6 μg/L for α-Fe2O3 and 17–21 μg/L for γ-Fe2O3 NPs (for 1 mg/L suspensions). Despite that, Fe levels in gills and brain reflect more dissolved Fe accumulation from metastable γ-Fe2O3 polymorph. Ingested NPs cleared from the organs completely within 30-day elimination period, except the liver and spleen. Liver contained about 31% of α- and 46% of γ-Fe2O3, while spleen retained about 62% of α- and 35% of the γ-polymorph. No significant disturbances were observed in hematological parameters, including hemoglobin, hematocrit, red blood cell and white blood cell counts (p > 0.05). Serum glucose (GLU) levels decreased in treatments exposed to 1.0 mg/L of γ-Fe2O3 NPs at day 30 (p < 0.05). In contrast, GLU levels increased during the elimination period for 1.0 mg/L α-Fe2O3 NPs treatments (p < 0.05). Transient increases occurred in glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and lactate dehydrogenase (LDH). Serum Fe levels did not change during exposure (p > 0.05), but increased significantly within elimination period due to mobilization of ingested NPs from liver and spleen to blood. Though respiratory burst activity was not affected (p > 0.05), lysozyme activity (LA) was suppressed suggesting an immunosuppressive effects from both Fe2O3 NPs (p < 0.05). In contrast, myeloperoxidase (MPO) levels increased significantly in treatments exposed to α-Fe2O3 NPs (p < 0.05), and the effect from γ-polymorph was marginal (p ≥ 0.05). The results indicate that morphological differences of Fe2O3 NPs could induce differential uptake, assimilation and immunotoxic effects on O. niloticus under chronic exposure.

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Section: Discussionsupporting

confidence: 72%

Chronic exposure of tilapia (Oreochromis niloticus) to iron oxide nanoparticles: Effects of particle morphology on accumulation, elimination, hematology and immune responses

et al. 2016

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“…In a study, McGeer et al () reported that suspensions of CuNPs caused hyperplasia, fusion and epithelial separations in the secondary lamella of the gills of rainbow trout. Similarly, exposure ZnO NPs caused inflections at the tips of secondary lamella, basal hyperplasia, fusion and epithelial separations in O. niloticus (Kaya et al, ). The changes identified in gill histopathology in this study are consistent with the existing literature.…”

Section: Resultsmentioning

confidence: 92%

“…Various fish species (e.g., rainbow trout, carp, and zebrafish) have been used as model animals to investigate toxicological effects of a number of metal and metal oxide NPs, such as Ag (Imani et al, ), Cu (Shaw et al, ; Sovová et al, ), ZnO (Hao & Chen, 2012, ; Ates et al, ; Kaya et al, ), CuO (Zhao et al, ; Ates et al, ), TiO 2 (Hao et al, ; Boyle et al ), Fe 2 O 3 (Remya et al, ; Saravanan et al ; Zhang et al ). To date, studies attempting to elucidate the adverse effects of ZnNPs have been conducted only recently by a handful of groups (Ates et al, 2103b; Abdel‐Khalek et al, 2015); therefore, there is a need for comprehensive studies detailing the ecotoxicological effects of ZnNPs.…”

Section: Introductionmentioning

confidence: 99%

Effects of subchronic exposure to zinc nanoparticles on tissue accumulation, serum biochemistry, and histopathological changes in tilapia (Oreochromis niloticus)

Kaya

Duysak

Akbulut

et al. 2016

Environmental Toxicology

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Zinc nanoparticles (ZnNPs) are among the least investigated NPs and thus their toxicological effects are not known. In this study, tilapia (Oreochromis niloticus) were exposed to 1 and 10 mg/L suspensions of small size (SS, 40–60 nm) and large size (LS, 80–100 nm) ZnNPs for 14 days under semi-static conditions. Total Zn levels in the intestine, liver, kidney, gill, muscle tissue and brain were measured. Blood serum glucose (GLU), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and lactate dehydrogenase (LDH) were examined to elucidate the physiological disturbances induced by ZnNPs. Organ pathologies were examined for the gills, liver and kidney to identify injuries associated with exposure. Significant accumulation was observed in the order of intestine, liver, kidney and gills. Zn levels exhibited time- and concentration-dependent increase in the organs. Accumulation in kidney was also dependent on particle size; NPs SS-ZnNPs were trapped more effectively than LS-ZnNPs. No significant accumulation occurred in the brain (p>0.05) while Zn levels in muscle tissue increased only marginally (p≥0.05). Significant disturbances were noted in serum GOT and LDH (p<0.05). The GPT levels fluctuated and were not statistically different from those of controls (p>0.05). Histopathological tubular deformations and mononuclear cell infiltrations were observed in kidney sections. In addition, an increase in melano-macrophage aggregation intensity was identified on the 7th day in treatments exposed to LS-ZnNPs. Mononuclear cell infiltrations were identified in liver sections for all treatments. Both ZnNPs caused basal hyperplasia in gill sections. Fusions appeared in the gills after the 7th day in fish treated with 10 mg/L suspensions of SS-ZnNPs. In addition, separations in the secondary lamella epithelia were observed. The results indicated that exposure to ZnNPs could lead to disturbances in blood biochemistry and cause histopathological injuries in the tissues of O. niloticus.

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“…Another part of blood was harvested in Z serum sep. tubes (MiniCollect ® Tube, Austria). Blood tubes were centrifuged at 4000x g for 10 min, and blood serum was separated (Kaya et al, 2016). Serum samples were stored at -20 °C and below until analysis (30 days).…”

Section: Blood Samplingmentioning

confidence: 99%

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Ozdemir¹,

Çelik²,

Yılmaz³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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A comparative toxicity study between small and large size zinc oxide nanoparticles in tilapia (Oreochromis niloticus): Organ pathologies, osmoregulatory responses and immunological parameters

Cited by 103 publications

References 59 publications

Chronic exposure of tilapia (Oreochromis niloticus) to iron oxide nanoparticles: Effects of particle morphology on accumulation, elimination, hematology and immune responses

Chronic exposure of tilapia (Oreochromis niloticus) to iron oxide nanoparticles: Effects of particle morphology on accumulation, elimination, hematology and immune responses

Effects of subchronic exposure to zinc nanoparticles on tissue accumulation, serum biochemistry, and histopathological changes in tilapia (Oreochromis niloticus)

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