A comparative study on the effects of ultrasound and some growth factors on somatic embryogenesis and artificial seed production in cucumber (Cucumis sativus L.)

Koochani, Morvarid; Majd, Ahmad; Arbabian, Sedigheh; Ghanati, Faezeh; Marandi, Sayeh Jafari

doi:10.15835/nbha48411669

Cited by 4 publications

(1 citation statement)

References 36 publications

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“…Plantlets were hardened and established in the field after being extracted from the encapsulated shoot tips. Furthermore, Koochani et al, [80] were able to develop artificia seeds using somatic embryos and apical buds of cucumber. The eggplant, a Solanaceae family member, is cultivated throughout the tropics as a vegetable crop and as a summer annual in the subtropics.…”

Section: Cucumber (Cucumis Sativus L)mentioning

confidence: 99%

Production of Synthetic Seeds in Vegetable Crops: A Review

Abbas

Mahood

Alhasan

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Over the last few decades, biotechnology research has advanced to the point where it is now possible to improve essential agricultural crops. Synthetic seed manufacturing has opened up new possibilities of in vitro plant propagation technique since it has various practical benefits on a commercial scale for the cultivation of a wide range of agricultural crops. It is one of the most important tools for plant tissue culture breeders and scientists, since it provides significant benefits for massive production of distinct (elite) plant species. Synthetic seeds offer a promising strategy for mass plant production in a way of encapsulation of non-embryonic or embryonic (somatic embryos) tissues in a gel-like matrix, such as shoot buds, shoot tips, microshoots, protocoms and nodal segments. When the strategies minimize the cost of the production of elite plant genotypes, this strategy can be adapted for commercial production. The technology of the synthetic seed necessitates the use of in vitro growth systems in order to produce viable materials that can be converted into plants on a wide scale. The discovery of an artificial seed technology opens up new possibilities of a promising strategy for improving a wide range of commercially significant plant species, including cereals, fruit crops, medicinal plants, and vegetable crops, which we focus on in this review article.

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Section: Cucumber (Cucumis Sativus L)mentioning

confidence: 99%

Production of Synthetic Seeds in Vegetable Crops: A Review

Abbas

Mahood

Alhasan

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Enhancing in vitro regeneration via somatic embryogenesis and Fusarium wilt resistance of Egyptian cucumber (Cucumis sativus L.) cultivars

Hamza,

Diab,

Khatab

et al. 2024

Journal of Genetic Engineering and Biotechnology

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Protocol for plant regeneration from encapsulated somatic embryos of Citrus sinensis L.

et al. 2023

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Background Citrus is an important fruit crop worldwide; artificial seeds are through encapsulation techniques of somatic embryos having many applications such as large-scale propagation and germplasm conservation. The aim of this study is the investigation of the viability of encapsulated citrus somatic embryos after different storage periods and to convert them into plantlets. Results Cotyledonary-stage somatic embryos (5–7-mm size) regenerated from stigma explants of Washington navel orange (Citrus sinensis L.) were encapsulated individually in 3% sodium alginate. After different preservation periods (1, 2 or 3 months) at 10 °C, the encapsulated somatic embryos were cultured on Murashige and Skooge (MS) medium solidified with 7 g/l agar and supplemented with 50 g/l sucrose for germination. Percentage of regrowth, germination percentage, number of plantlets, plantlets height and number of leaves/shoot were recorded after different recovery periods. The germination percentages of encapsulated embryos were 90, 62.5 and 40% with storage for one, two and three months, respectively. Encapsulated somatic embryos preserved for 1 month developed the highest number of plantlets, while those preserved for 2 months developed the highest length of plantlets and the highest number of leaves/shoot after a recovery period of 10 months. Molecular analysis was performed of plantlets recovered from somatic embryos after preservation by encapsulation, and the results showed that the percentages of polymorphism were 7.7% with the two primers in all treatments. Conclusions It could be concluded that callus developed from stigma explants was able to regenerate indirect somatic embryogenesis after 3 months. Cotyledonary-stage somatic embryos of citrus were successfully preserved by encapsulation using 3% sodium alginate. Frequencies of germination of encapsulated somatic embryos increased with increasing the recovery period on the germination medium. A maximum recovery frequency of 60.8% was obtained from encapsulated somatic embryos cultured on germination medium for up to 10 months. Also, recovery frequency of 62.5% was noticed from encapsulated somatic embryos preserved for 2 months at 10 °C. In addition, results indicated that recovered somatic embryos obtained from encapsulated somatic embryos were able to convert to normal plantlets.

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A comparative study on the effects of ultrasound and some growth factors on somatic embryogenesis and artificial seed production in cucumber (Cucumis sativus L.)

Cited by 4 publications

References 36 publications

Production of Synthetic Seeds in Vegetable Crops: A Review

Production of Synthetic Seeds in Vegetable Crops: A Review

Enhancing in vitro regeneration via somatic embryogenesis and Fusarium wilt resistance of Egyptian cucumber (Cucumis sativus L.) cultivars

Protocol for plant regeneration from encapsulated somatic embryos of Citrus sinensis L.

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