A comparative study on the composition and importance of free amino acids in semen of gilthead sea bream, Sparus aurata, and perch, Perca fluviatilis

Lahnsteiner, Franz

doi:10.1007/s10695-010-9442-3

Cited by 15 publications

(16 citation statements)

References 27 publications

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“…Amino acids are also oxidisable substrates for sperm and can bind toxic heavy metals, thus preserving the viability of spermatozoa (Chaudhury, Sharma, Jagannathan, & Guha, ; Papp, Gróf, & Menyhárt, ; Patel, Srivastava, Phadke, & Govil, ). Lahnsteiner () reported that valine has positive effect on sperm motility in Perca fluviatilis . Our experiment confirmed that deficiency of valine in seminal plasma may contribute to the poor sperm motility of asthenozoospermic men.…”

Section: Discussionmentioning

confidence: 99%

Metabonomic analysis of fatty acids in seminal plasma between healthy and asthenozoospermic men based on gas chromatography mass spectrometry

Tang

Shang

et al. 2017

Andrologia

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The mechanism of asthenozoospermia remains unclear. The knowledge of the metabolism of fatty acids in seminal plasma is important and meaningful for the pathological study of asthenozoospermia. We present an optimised assay of extraction and derivatisation followed by GC/MS to analyse metabolites, especially fatty acids, in seminal plasma from healthy and asthenozoospermic men. Eighty-nine peaks including 17 kinds of fatty acids were analysed and identified in the chromatogram. The GC/MS data were analysed using t test, fold change and partial least squares discriminant analysis to explore the potential biomarkers of asthenozoospermia. Seven metabolites in asthenozoospermic group were found to be significantly different from those in the normal group (with p < .05, fold change >1.2 and variable importance for projection >1). Of which, high levels of oleic acid and palmitic acid in seminal plasma from asthenozoospermic men may indicate a membrane metabolism disorder in spermatozoa and the lack of valine in the asthenozoospermic group may contribute to poor sperm motility. The results may facilitate the understanding of the role of fatty acids and amino acids in asthenozoospermia and provide solid foundation for further pathological study of asthenozoospermia.

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Section: Discussionmentioning

confidence: 99%

Metabonomic analysis of fatty acids in seminal plasma between healthy and asthenozoospermic men based on gas chromatography mass spectrometry

Tang

Shang

et al. 2017

Andrologia

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“…Lysine, threonine, tryptophan, and valine are essential amino acids, and their levels in plasma were seriously affected by food intake. Good sources of these amino acids are high-protein foods such as animal products [ 48 , 49 ]. Hydroxyproline is a non-proteinogenic amino acid, which is produced by the hydroxylation of proline.…”

Section: Discussionmentioning

confidence: 99%

Meat and Seafood Consumption in Relation to Plasma Metabolic Profiles in a Chinese Population: A Combined Untargeted and Targeted Metabolomics Study

Zou

et al. 2017

Nutrients

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We examined the relationship between different patterns of meat and seafood consumption and plasma metabolic profiles in an Asian population. We selected 270 ethnic Chinese men and women from the Singapore Prospective Study Program based on their dietary habits assessed with a validated food frequency questionnaire. Participants were divided into four subgroups: high meat and high seafood (n = 60), high meat and low seafood (n = 64), low meat and high seafood (n = 60), and low meat and low seafood (n = 86) consumers. Plasma metabolites were measured using both targeted and untargeted mass spectroscopy-based analyses. A total of 42 metabolites differed significantly by dietary group. Higher concentrations of essential amino acids, polyunsaturated fatty acids, and d-glucose were found in high meat and/or seafood consumers as compared with the group with a low consumption of these animal foods. Red meat, poultry, fish, shellfish, soy products, and dairy were each correlated with at least one differential metabolite (r = −0.308 to 0.448). Some observations, such as the correlation between fish and 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF), confirmed previous studies. Other observations, such as the correlation between shellfish and phosphatidylethanolamine (p36:4), were novel. We also observed significant correlations between plasma metabolites and clinical characteristics, such as CMPF with fasting blood glucose (r = 0.401). These findings demonstrate a significant influence of meat and seafood consumption on metabolic profiles in the Asian population.

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“…Arginine was the main free amino acid in the spermatozoa of Oncorhynchus mykis s, Cyprinus carpio and Perca fluviatilis . In addition, arginine, glutamic acid and methionine were found in the semen of several fish species where sperm motility was improved when spermatozoa were co-incubated with methionine [ 3 , 4 ]. In mouse, cysteine analogs can increase the fertilization of frozen-thawed sperms [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway

Zhang

Liu

et al. 2017

Oncotarget

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Amino acid supplementation is an efficient and effective strategy to increase sperm quality. In our research, a comparative study was conducted to screen free amino acids to improve sperm motility, and we found that leucine was the most efficient one. Leucine treatment increases sperm motility depending on the activation of PI3K/Akt signaling pathway, while the chemical inhibitor of PI3K/Akt signal could reduce the amount of pAkt activated by leucine treatment. Moreover, leucine treatment improved the expression of P62 and LC3-II, substantially suppressed the autophagy process in zebrafish testis. In vitro studies showed that leucine could reduce the fusion of autophagosome and lysosome that was indicated by the co-localization of EGFP-LC3 and lysosome marker. Two chemical modulators of autophagy, such as LY294002 (the inhibitor of PI3K/Akt signal) and chloroquine were administered to investigate the process of autophagy on zebrafish sperm motility. LY294002 inhibited autophagosome formation to reduced sperm motility, while chloroquine inhibited the fusion of autophagosome and lysosome to improve sperm motility. Our data suggest that short-term treatment with leucine could increase zebrafish sperm motility by affecting the autophagy and inhibiting the fusion of autophagosome and lysosomes, depending on the activation of PI3K/Akt signaling pathway.

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A comparative study on the composition and importance of free amino acids in semen of gilthead sea bream, Sparus aurata, and perch, Perca fluviatilis

Cited by 15 publications

References 27 publications

Metabonomic analysis of fatty acids in seminal plasma between healthy and asthenozoospermic men based on gas chromatography mass spectrometry

Metabonomic analysis of fatty acids in seminal plasma between healthy and asthenozoospermic men based on gas chromatography mass spectrometry

Meat and Seafood Consumption in Relation to Plasma Metabolic Profiles in a Chinese Population: A Combined Untargeted and Targeted Metabolomics Study

Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway

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