A comparative study on the transformation of Aspergillus nidulans by microprojectile bombardment of conidia and a more conventional procedure using protoplasts treated with polyethyleneglycol

Herzog, Roland W.; Daniell, Henry; Singh, Narendra K.; Lemke, Paul A.

doi:10.1007/s002530050692

Cited by 42 publications

(30 citation statements)

References 20 publications

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“…Direct comparisons of these results might be insignificant in the light of different protocols, and fungal genera and the selection markers used in the biolistic transformations. Herzog et al 30) found that the transformation frequency of Aspergillus nidulans was higher under PEG/CaCl 2 electroporation than under particle bombardment, but the rate of stable transformation was considerably higher with particle bombardment. Similarly, Lorito et al 29) found with Trichoderma harzianum and Gliocladium virens that the biolistic procedure produced genetically stable progeny as compared with protoplast-mediated transformation.…”

Section: )mentioning

confidence: 99%

Transformation of the Mycorrhizal Basidiomycetes,Suillus grevilleiandS. bovinus, by Particle Bombardment

Sunagawa

Murata

Miyazaki

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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Mycorrhizal basidiomycetes, Suillus grevillei and S. bovinus, were transformed by particle bombardment. We isolated eight and four transformants from S. grevillei and S. bovinus respectively under three conditions differing in terms of target distance and helium pressure. The transformation frequencies were 1.2 and 0.6 transformants/g DNA. Plasmid DNAs were integrated into the genomic DNA of the transformants and stably maintained. This is the first description of the transformation of S. grevillei and S. bovinus by particle bombardment.

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Section: )mentioning

confidence: 99%

Transformation of the Mycorrhizal Basidiomycetes,Suillus grevilleiandS. bovinus, by Particle Bombardment

Sunagawa

Murata

Miyazaki

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…This result indicated that the DNA transferred by particle bombardment was stably maintained in the mycelium. The 100% mitotic stability of the fi ve transformants was higher than the 60%-70% of A. nidulans (Herzog et al 1996) achieved by particle bombardment. In addition, Hazell et al (2000) and Te'o et al (2002) obtained results similar to ours.…”

Section: Abstract Lyophyllum Decastes · Particle Bombardment · Transmentioning

confidence: 83%

“…Transformation frequency is affected by the nature (gold and tungsten) and size of particles, the distance from the mycelial mat to the particles, and the bombardment pressure. Herzog et al (1996) compared particle bombardment and PEG/CaCl 2 treatment of A. nidulans. They reported that the transformation frequency with the former was somewhat lower than that with the latter; however, the rate of stable transformation was considerably higher with particle bombardment.…”

Section: Abstract Lyophyllum Decastes · Particle Bombardment · Transmentioning

confidence: 99%

Transformation of Lyophyllum decastes by particle bombardment

Sunagawa¹,

Murata²,

Miyazaki³

et al. 2007

Mycoscience

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“…Examples of fungal species of which the genome has been transformed by biolistic bombardment include "academic" fungi such as Aspergillus nidulans (Herzog et al 1996 ) and Neurospora crassa (Armaleo et al 1990 ) and the high protein secreting industrial "workhorse" Trichoderma reesei (Hazell et al 2000 ;Te'o et al 2002 ), amongst others.…”

Section: Introductionmentioning

confidence: 98%