Transformation of Lyophyllum decastes by particle bombardment

Sunagawa, Masahide; Murata, Hitoshi; Miyazaki, Yasumasa; Nakamura, Masaya

doi:10.1007/s10267-007-0345-9

Cited by 4 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LiAc and ssDNA also increase the permeability of nucleic acids, although the detailed mechanism is not fully understood [ 49 , 50 ]. PEG treatment has been applied to the transformation of Pleurotus mushrooms using PEG3350 or PEG4000, as shown in Table 2 [ 38 , 39 , 41 , 43 , 44 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ]. Electroporation is a method for local disturbance of the cell membrane by applying electric pulses with a certain electric potential (V) and capacitance (F).…”

Section: Methods For Mushroom Transformationmentioning

confidence: 99%

“…Ballistic bombardment is a transformation method using helium pressure to introduce DNA-coated gold particles into cells. This method has been generally applied for the transient expression of genes in plants, and it has been seldom applied for mushroom transformation [ 57 , 58 ]. Agrobacterium tumefaciens can make holes in the membrane of any living organism.…”

Section: Methods For Mushroom Transformationmentioning

confidence: 99%

See 1 more Smart Citation

Current Technologies and Related Issues for Mushroom Transformation

Kim

2015

Mycobiology

View full text Add to dashboard Cite

Mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector DNA, removal of host cell wall, introduction of foreign DNA across the cell membrane, and integration into host genomic DNA or maintenance of an autonomous vector DNA inside the host cell. This review introduces limitations and obstacles related to transformation technologies along with possible solutions. Current methods for cell wall removal and cell membrane permeabilization are summarized together with details of two popular technologies, Agrobacterium tumefaciens-mediated transformation and restriction enzyme-mediated integration.

show abstract

Section: Methods For Mushroom Transformationmentioning

confidence: 99%

Section: Methods For Mushroom Transformationmentioning

confidence: 99%

Current Technologies and Related Issues for Mushroom Transformation

Kim

2015

Mycobiology

View full text Add to dashboard Cite

show abstract

“…However, the molecular mechanism underlining the biochemical evidence was still unknown. Sunagawa et al firstly reported the transformation of L. decastes ( Sunagawa et al, 2007 ). The pHHM192 plasmid containing the hygromycin B reporter gene was transformed into L. decastes N1 strain by particle bombardment.…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequences and comparative secretomic analysis for the industrially cultivated edible mushroom Lyophyllum decastes reveals insights on evolution and lignocellulose degradation potential

Yang²,

Qiu

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Lyophyllum decastes, also known as Luronggu in China, is a culinary edible and medicinal mushroom that was widely cultivated in China in recent years. In the present study, the complete high-quality genome of two mating compatible L. decastes strain was sequenced. The L. decastes LRG-d1-1 genome consists of 47.7 Mb in 15 contigs with a contig N90 of 2.08 Mb and 14,499 predicted gene models. Phylogenetic analysis revealed that L. decastes exhibits a close evolutionary relationship to the Termitomyces and Hypsizygus genus and was diverged from H. marmoreus ~ 45.53 Mya ago. Mating A loci of L. decastes compose of five and four HD genes in two monokaryotic strains, respectively. Mating B loci compose of five STE genes in both two monokaryotic strains. To accelerate the cross-breeding process, we designed four pairs of specific primers and successfully detected both mating types in L. decastes. As a wood-rotting mushroom, a total of 541 genes accounting for 577 CAZymes were identified in the genome of L. decastes. Proteomic analysis revealed that 1,071 proteins including 182 CAZymes and 258 secreted enzymes were identified from four groups (PDB, PDB + bran, PDB + cotton hull, and PDB + sawdust). Two laccases and a quinone reductase were strongly overproduced in lignin-rich cultures, and the laccases were among the top-3 secreted proteins, suggesting an important role in the synergistic decomposition of lignin. These results revealed the robustness of the lignocellulose degradation capacity of L. decastes. This is the first study to provide insights into the evolution and lignocellulose degradation of L. decastes.

show abstract

“…Its agricultural, commercial, and medical value make it an attractive option for consumers worldwide, with significant potential for growth and economic benefit in the fields of industry, medicine, and agriculture ( Ukawa et al, 2007 ). Previous research on L. decastes mainly focused on disease prevention, genetics and bioactive substances ( Pokhrel & Ohga, 2007 ; Sunagawa et al, 2008 ; Parada et al, 2011 ; Clericuzio et al, 2013 ). In spite of the critical role played by the fully developed fruiting body of L. decastes in commercial and medicinal applications, there exists a notable paucity of information concerning the molecular mechanism that governs its growth and development.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis on candidate genes associated with fruiting body growth and development in Lyophyllum decastes

Ke,

Ding,

Niu

et al. 2023

PeerJ

View full text Add to dashboard Cite

Lyophyllum decastes is a mushroom that is highly regarded for its culinary and medicinal properties. Its delectable taste and texture make it a popular choice for consumption. To gain a deeper understanding of the molecular mechanisms involved in the development of the fruiting body of L. decastes, we used RNA sequencing to conduct a comparative transcriptome analysis. The analysis encompassed various developmental stages, including the vegetative mycelium, primordial initiation, young fruiting body, medium-size fruiting body, and mature fruiting body stages. A range of 40.1 to 60.6 million clean reads were obtained, and de novo assembly generated 15,451 unigenes with an average length of 1,462.68 bp. Functional annotation of transcriptomes matched 76.84% of the unigenes to known proteins available in at least one database. The gene expression analysis revealed a significant number of differentially expressed genes (DEGs) between each stage. These genes were annotated and subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. Highly differentially expressed unigenes were also identified, including those that encode extracellular enzymes, transcription factors, and signaling pathways. The accuracy of the RNA-Seq and DEG analyses was validated using quantitative PCR. Enzyme activity analysis experiments demonstrated that the extracellular enzymes exhibited significant differences across different developmental stages. This study provides valuable insights into the molecular mechanisms that underlie the development of the fruiting body in L. decastes.

show abstract

Transformation of Lyophyllum decastes by particle bombardment

Cited by 4 publications

References 23 publications

Current Technologies and Related Issues for Mushroom Transformation

Current Technologies and Related Issues for Mushroom Transformation

Complete genome sequences and comparative secretomic analysis for the industrially cultivated edible mushroom Lyophyllum decastes reveals insights on evolution and lignocellulose degradation potential

Comparative transcriptome analysis on candidate genes associated with fruiting body growth and development in Lyophyllum decastes

Contact Info

Product

Resources

About