A comparative study on morphological versus molecular identification of dermatophyte isolates

Ahmadi, Bahram; Mirhendi, Hossein; Shidfar, Mohammad Reza; Nouripour‐Sisakht, Sadegh; Jalalizand, Nilufar; Geramishoar, Mohsen; Shokoohi, Gholamreza

doi:10.1016/j.mycmed.2014.10.022

Cited by 38 publications

(31 citation statements)

References 31 publications

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“…In addition to ITS regions, other gene encoding sequencing regions have also been utilized to identify fungal species including β-tubulin, calmodulin, and the mitochondrial gene CO1 [37, 39]. Targeting the ITS region in surveys of indoor and occupational environments has improved the identification of fungi compared to culture-dependent approaches where morphological examination can result in misidentifications [40, 41]. However, several sequence amplification considerations within the analysis matrix could introduce selection biases and should be considered before utilizing ITS region sequencing methods.…”

Section: Its Region Sequencingmentioning

confidence: 99%

Emerging Insights into the Occupational Mycobiome

Green

2018

Curr Allergy Asthma Rep

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Purpose of Review The evolution of molecular-based methods over the last two decades has provided new approaches to identify and characterize fungal communities or “mycobiomes” at resolutions previously not possible using traditional hazard identification methods. The recent focus on fungal community assemblages within indoor environments has provided renewed insight into overlooked sources of fungal exposure. In occupational studies, internal transcribed spacer (ITS) region sequencing has recently been utilized in a variety of environments ranging from indoor office buildings to agricultural commodity and harvesting operations. Recent Findings Fungal communities identified in occupational environments have been primarily placed in the phylum Ascomycota and included classes typically identified using traditional fungal exposure methods such as the Eurotiomycetes, Dothideomycetes, Sordariomycetes, and Saccharomycetes. The phylum Basidiomycota has also been reported to be more prevalent than previously estimated and ITS region sequences have been primarily derived from the classes Agaricomycetes and Ustilaginomycetes. These studies have also resolved sequences placed in the Basidiomycota classes Tremellomycetes and Exobasidiomycetes that include environmental and endogenous yeast species. Summary These collective datasets have shown that occupational fungal exposures include a much broader diversity of fungi than once thought. Although the clinical implications for occupational allergy are an emerging field of research, establishing the mycobiome in occupational environments will be critical for future studies to determine the complete spectrum of worker exposures to fungal bioaerosols and their impact on worker health.

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Section: Its Region Sequencingmentioning

confidence: 99%

Emerging Insights into the Occupational Mycobiome

Green

2018

Curr Allergy Asthma Rep

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“…Consequently, the investigation of ITS region by sequencing method was provided with a simple technique for fungal characterization [32]. The diverse length of ITS region among fungal species confirmed the results of laboratory conventional identification [33,34]. The finding PCR product was sequenced according to Macrogen company/ Korea using one primer ITS1 and our sequence was placed in GenBank with accession no.…”

Section: Discussionmentioning

confidence: 70%

Molecular Identification of Fusarium proliferatum from toenails: A case report

Gharib

Tahir

2019

KJAR

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Fusarium is a saprophytic fungus which is broadly disseminated in soil, plant debris and other organic substrates. These fungi can affect indigenous tissue in immunocompromised patients and result in some fungal infections such as Onychomycosis, bone and joint disease, or sinusitis. In this paper, we report a 56-year-old female has Diabetes milletus who established to disseminate Fusarium infection in her left first toenail from the Dermatology Department / Khabat Skin Center, Sulaimani Province, Iraq. To study of these fungi as the true causative agents of nail infection, we describe a PCR/sequencing assay to confirm routinely diagnosis of the infecting fungi in this case with toenail Onychomycosis. Designation of the ordinary laboratory of Fusarium is very challenging because of the septate hyphae of Fusarium are hard to discriminate from those of Aspergillus, which has a more favourable consequence and this time-consuming. Therefore, molecular tools were used for identification of F. proliferatum from this case by amplifying ITS region of rDNA using a pair of universal primers ITS1 and ITS4. Infectious fungal molecular detection advances the select of right antifungal therapy, in that way improving the medication rate of nails infections. DNA sequence analysis and Phylogenetic tree of the amplified ITS-rDNA was used for species identification of this strain of F. proliferatum. For final identification sequencing of DNA has been carried out by [Macrogen Korea] using forward primer ITS1, The obtained consensus of query sequence was compared with the ITS DNA database on the BLAST homepage, aligned and diagnosed strain deposited to the GenBank and we received an accession number (MK112619). The sequence of the F. proliferatum amplicon was aligned using ClustalW and the alignment was used to make phylogenetic analysis using MEGA software version X. To the best of our knowledge, the isolated Fusarium in this paper is the first case of its kind to be sequenced and reported by the molecular method in Kurdistan region of Iraq

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“…Furthermore, since all T rubrum strains tested in our study had only recently been isolated from active tinea lesions, it is clear that they all were equipped with the physiological capacities necessary to induce active infections in humans. This is a major advantage to strains obtained from collections, because the physiological properties of dermatophytes tend to dwindle away in the course of prolonged storage and cultivation because of pleomorphism . The use of clinical isolates therefore better models the clinical situation.…”

Section: Discussionmentioning

confidence: 99%

“…This is a major advantage to strains obtained from collections, because the physiological properties of dermatophytes tend to dwindle away in the course of prolonged storage and cultivation because of pleomorphism. [37][38][39] The use of clinical isolates therefore better models the clinical situation. Our control tests with terbinafine and itraconazole revealed MICs within the range to be expected for T rubrum 40,41 ; only 1 strain showed a rather high MIC (8 mg/L) for itraconazole.…”

Section: Discussionmentioning

confidence: 99%

Clinical isolates of Trichophyton rubrum are completely inhibited by photochemical treatment with a γ‐cyclodextrin formulation of curcuminoids

Brasch

Beck-Jendroschek

Walther

et al. 2020

Mycoses

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Summary Introduction It was shown previously that dermatophytes can markedly be inhibited by a photochemical treatment with curcumin. This kind of photo‐inactivation needs to be improved, however, because curcumin is poorly water‐soluble. Therefore, a new water‐soluble γ‐cyclodextrin formulation of curcuminoids was tested for its photochemical inactivation of Trichophyton (T.) rubrum. Materials and methods Conidia were harvested from 6 typical strains of T rubrum and used to inoculate wells of microtiter plates. These wells were also filled with a γ‐cyclodextrin curcuminoid formulation with 0.1% DMSO and Sabouraud broth. The assays were then irradiated with visible light (wavelength 420 nm, 45 J/cm2). After 24 hours, curcuminoid was added once more, and irradiation was repeated. Fungal growth was monitored photometrically for 8 days and compared with controls. Results Growth of all 6 T rubrum strains was completely inhibited by the photochemical treatment with the γ‐cyclodextrin formulation of curcuminoids. The same curcuminoid formulation applied without irradiation had only a minor inhibitory effect. Discussion Photo‐inactivation of dermatophytes with a γ‐cyclodextrin formulation of curcuminoids plus visible light is a very promising procedure with potential for a new treatment of patients with superficial tinea.

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A comparative study on morphological versus molecular identification of dermatophyte isolates

Cited by 38 publications

References 31 publications

Emerging Insights into the Occupational Mycobiome

Emerging Insights into the Occupational Mycobiome

Molecular Identification of Fusarium proliferatum from toenails: A case report

Clinical isolates of Trichophyton rubrum are completely inhibited by photochemical treatment with a γ‐cyclodextrin formulation of curcuminoids

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