Clinical isolates of Trichophyton rubrum are completely inhibited by photochemical treatment with a γ‐cyclodextrin formulation of curcuminoids

Abstract

Summary Introduction It was shown previously that dermatophytes can markedly be inhibited by a photochemical treatment with curcumin. This kind of photo‐inactivation needs to be improved, however, because curcumin is poorly water‐soluble. Therefore, a new water‐soluble γ‐cyclodextrin formulation of curcuminoids was tested for its photochemical inactivation of Trichophyton (T.) rubrum. Materials and methods Conidia were harvested from 6 typical strains of T rubrum and used to inoculate wells of microtiter plates. These wells were also filled with a γ‐cyclodextrin curcuminoid formulation with 0.1% DMSO and Sabouraud broth. The assays were then irradiated with visible light (wavelength 420 nm, 45 J/cm2). After 24 hours, curcuminoid was added once more, and irradiation was repeated. Fungal growth was monitored photometrically for 8 days and compared with controls. Results Growth of all 6 T rubrum strains was completely inhibited by the photochemical treatment with the γ‐cyclodextrin formulation of curcuminoids. The same curcuminoid formulation applied without irradiation had only a minor inhibitory effect. Discussion Photo‐inactivation of dermatophytes with a γ‐cyclodextrin formulation of curcuminoids plus visible light is a very promising procedure with potential for a new treatment of patients with superficial tinea.

Antimicrobial Activity of Curcumin in Nanoformulations: A Comprehensive Review

“…When the formulation was used as a PS, aPDT resulted in complete growth inhibition of all strainS. No mycelium was observed in samples submitted to aPDT [140].…”

“…The antifungal effect of a formulation of γ-CD with curcuminoids (65-82% CUR, 15-25% demethoxycurcumin, and 2-7% bisdemethoxycurcumin) was evaluated against six strains of the dermatophyte Trichophyton rubrum isolated from active tinea lesions [140]. The MIC of terbinafine was <0.06 mg/L while the MIC of itraconazole ranged from ≤1 to 8 mg/L for all strainS.…”