A comparative study on cytotoxicity and genotoxicity of the hydroxyapatite‐bioactive glass and fluorapatite‐bioactive glass nanocomposite foams as tissue scaffold for bone repair

Seyedmajidi, Seyedali; Seyedmajidi, Maryam; Zabihi, Ebrahim; Hajian‐Tilaki, Karimollah

doi:10.1002/jbm.a.36452

Cited by 16 publications

(11 citation statements)

References 22 publications

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“…As a traditional coating material for dental implants, HA is widely recognized as a type of bioactive material with good biocompatibility and bone conductivity. A large number of studies have reported that HA has good biocompatibility and can meet the requirements of implant materials, which is consistent with the results of our study [ 33 , 34 ]. Plasma sprayed HA-coated implants are commonly used.…”

Section: Discussionsupporting

confidence: 93%

Tantalum Particles Induced Cytotoxic and Inflammatory Effects in Human Monocytes

Yang

Zhang

Kang

et al. 2021

BioMed Research International

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The aim of this study is to evaluate the biological safety of tantalum (Ta) particles and to further explore the effects of Ta particles on human monocyte toxicity and inflammatory cytokine expression. Human monocyte leukemia (THP-1) cells were cultured with Ta and hydroxyapatite (HA) particles. Cell counting kit-8 method was used to evaluate the cytotoxicity of Ta and HA particles. The apoptosis effects were evaluated by flow cytometry, and the protein expression levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were evaluated by ELISA. The protein levels of inflammation-related signaling pathways including nuclear factor-kappa B (NF-κB) and extracellular regulated kinase (ERK) were detected by western blotting. The cytotoxicity test showed that the toxicity level of Ta in vitro was grade l, which is within the clinically acceptable range. Compared with the HA control, Ta had no significant effect on THP-1 cell apoptosis, IL-6, and TNF-α release. The phosphorylated levels of NF-κB and ERK at 3 h in the Ta group were lower than those in the HA and control groups ( P < 0.001 both). These results reveal Ta particles behave good biosafety properties and provide some new insights for the future clinical use of Ta.

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Section: Discussionsupporting

confidence: 93%

Tantalum Particles Induced Cytotoxic and Inflammatory Effects in Human Monocytes

Yang

Zhang

Kang

et al. 2021

BioMed Research International

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“…The results obtained are in good agreement with studies presented by Cai et al (2007) who showed that hydroxyapatite crystals affects the metabolic activity of U-2 OS [56]. Additionally, Seyedmajidi et al [59] showed that hydroxyapatite/bioactive glass (HA/BG) at certain concentration may have a cytotoxic effect, and decrease metabolic potential of Saos-2. In turn, in this study we have shown that mitochondrial metabolism of MG-63 is accelerated in cultures on 10 wt % 3 mol % Eu 3+ : nHAp/PLLA scaffold.…”

Section: Discussionsupporting

confidence: 89%

The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells

et al. 2019

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Osteosarcoma (OSA) is malignant bone tumor, occurring in children and adults, characterized by poor prognosis. Despite advances in chemotherapy and surgical techniques, the survival of osteosarcoma patients is not improving significantly. Currently, great efforts are taken to identify novel selective strategies, distinguishing between cancer and normal cells. This includes development of biomimetic scaffolds with anticancer properties that can simultaneously support and modulate proper regeneration of bone tissue. In this study cytotoxicity of scaffolds composed from poly (L-lactic acid) functionalized with nanohydroxyapatite (nHAp) and doped with europium (III) ions—10 wt % 3 mol % Eu3+: nHAp@PLLA was tested using human osteosarcoma cells: U-2 OS, Saos-2 and MG-63. Human adipose tissue-derived stromal cells (HuASCs) were used as non-transformed cells to determine the selective cytotoxicity of the carrier. Analysis included evaluation of cells morphology (confocal/scanning electron microscopy (SEM)), metabolic activity and apoptosis profile in cultures on the scaffolds. Results obtained indicated on high cytotoxicity of scaffolds toward all OSA cell lines, associated with a decrease of cells’ viability, deterioration of metabolic activity and activation of apoptotic factors determined at mRNA and miRNA levels. Simultaneously, the biomaterials did not affect HuASCs’ viability and proliferation rate. Obtained scaffolds showed a bioimaging function, due to functionalization with luminescent europium ions, and thus may find application in theranostics treatment of OSA.

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“…Seyedmadiji et al [85] investigated the functionality of HA/bioactive glass (BG) and fluorapatite (FA)/BG materials. They also employed the Comet assay to investigate potential genotoxic effects on Saos-2 cells and found a dose-dependent increase in DNA degradation, but within the limits of safety (therefore, below any threshold of genotoxicity).…”

Section: Biocompatibility Assessment Of Cap-based Bioceramicsmentioning

confidence: 99%

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

et al. 2019

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Recently, a large spectrum of biomaterials emerged, with emphasis on various pure, blended, or doped calcium phosphates (CaPs). Although basic cytocompatibility testing protocols are referred by International Organization for Standardization (ISO) 10993 (parts 1–22), rigorous in vitro testing using cutting-edge technologies should be carried out in order to fully understand the behavior of various biomaterials (whether in bulk or low-dimensional object form) and to better gauge their outcome when implanted. In this review, current molecular techniques are assessed for the in-depth characterization of angiogenic potential, osteogenic capability, and the modulation of oxidative stress and inflammation properties of CaPs and their cation- and/or anion-substituted derivatives. Using such techniques, mechanisms of action of these compounds can be deciphered, highlighting the signaling pathway activation, cross-talk, and modulation by microRNA expression, which in turn can safely pave the road toward a better filtering of the truly functional, application-ready innovative therapeutic bioceramic-based solutions.

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A comparative study on cytotoxicity and genotoxicity of the hydroxyapatite‐bioactive glass and fluorapatite‐bioactive glass nanocomposite foams as tissue scaffold for bone repair

Cited by 16 publications

References 22 publications

Tantalum Particles Induced Cytotoxic and Inflammatory Effects in Human Monocytes

Tantalum Particles Induced Cytotoxic and Inflammatory Effects in Human Monocytes

The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

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