A comparative study on cockroach and ovalbumin sensitizations and challenge responses in Hartley guinea-pigs

Chen, Gang; Zhou, Daohong; Kang, Bann C.

doi:10.1016/s0034-5687(00)00222-x

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…Moreover, the combination of intraperitoneal and subcutaneous injections together with aerosol boosters cause EAR and OVA-specific IgG 1 and IgE, besides AHR and eosinophilia, but no LAR [39,107]. Several other compounds have been used as allergens in guinea pig models, such as HDM extracts [108,109], purified proteins from mites [109], cockroach extracts [110][111][112], and trimellitic anhydride dust [113,114]. However, there have been very few models developed and AHR was not established.…”

Section: The Allergic Responsementioning

confidence: 99%

Back to the future: re-establishing guinea pigin vivoasthma models

et al. 2020

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Research using animal models of asthma is currently dominated by mouse models. This has been driven by the comprehensive knowledge on inflammatory and immune reactions in mice, as well as tools to produce genetically modified mice. Many of the identified therapeutic targets influencing airway hyper-responsiveness and inflammation in mouse models, have however been disappointing when tested clinically in asthma. It is therefore a great need for new animal models that more closely resemble human asthma. The guinea pig has for decades been used in asthma research and a comprehensive table of different protocols for asthma models is presented. The studies have primarily been focused on the pharmacological aspects of the disease, where the guinea pig undoubtedly is superior to mice. Further reasons are the anatomical and physiological similarities between human and guinea pig airways compared with that of the mouse, especially with respect to airway branching, neurophysiology, pulmonary circulation and smooth muscle distribution, as well as mast cell localization and mediator secretion. Lack of reagents and specific molecular tools to study inflammatory and immunological reactions in the guinea pig has however greatly diminished its use in asthma research. The aim in this position paper is to review and summarize what we know about different aspects of the use of guinea pig in vivo models for asthma research. The associated aim is to highlight the unmet needs that have to be addressed in the future.

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Section: The Allergic Responsementioning

confidence: 99%

Back to the future: re-establishing guinea pigin vivoasthma models

et al. 2020

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“…Clinically, it has been shown that the rate of hospitalization, the number of days missed from school due to asthma and the number of days of wheezing were all significantly higher in asthmatic children sensitive to cockroach allergen compared to asthmatic children who are not [2]. Experimental models comparing the responses of guinea‐pigs sensitized to ovalbumin or cockroach allergen showed that animals sensitized to cockroach allergen had increased airway resistance that persisted far longer than those sensitized to ovalbumin and the cockroach allergen sensitized animals had significantly higher pulmonary cellular influx compared to ovalbumin sensitized animals [3]. Environmentally, asthmatic subjects are exposed to an infinite number of allergens and particulates which may affect the morbidity of disease.…”

Section: Introductionmentioning

confidence: 99%

Reproducibility of a novel model of murine asthma-like pulmonary inflammation

McKinley

Kim

Bolgos

et al. 2004

Clinical and Experimental Immunology

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SUMMARYSensitization to cockroach allergens (CRA) has been implicated as a major cause of asthma, especially among inner-city populations. Endotoxin from Gram-negative bacteria has also been investigated for its role in attenuating or exacerbating the asthmatic response. We have created a novel model utilizing house dust extract (HDE) containing high levels of both CRA and endotoxin to induce pulmonary inflammation (PI) and airway hyperresponsiveness (AHR). A potential drawback of this model is that the HDE is in limited supply and preparation of new HDE will not contain the exact components of the HDE used to define our model system. The present study involved testing HDEs collected from various homes for their ability to cause PI and AHR. Dust collected from five homes was extracted in phosphate buffered saline overnight. The levels of CRA and endotoxin in the supernatants varied from 7·1 to 49·5 mg/ml of CRA and 1·7-6 m g/ml of endotoxin in the HDEs. Following immunization and two pulmonary exposures to HDE all five HDEs induced AHR, PI and plasma IgE levels substantially higher than normal mice. This study shows that HDE containing high levels of cockroach allergens and endotoxin collected from different sources can induce an asthma-like response in our murine model.

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“…Therefore, better models such as cockroach allergen (e.g. Blag2) (Chen, Zhou, & Kang, 2001) or house dust mite allergen (Ahn et al, 2007) may be used in the further study of ours.…”

Section: Establishment Of Dehp-ova-asthma Rat Modelmentioning

confidence: 99%

Adjuvant effect of di-(2-ethylhexyl) phthalate on asthma-like pathological changes in ovalbumin-immunised rats

Yang

Qiao

et al. 2008

Food and Agricultural Immunology

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Pollution of phthalates, including di-(2-ethylhexyl)phthalate (DEHP), is widespread because of their use in plastics and other daily consumer products. Epidemiological studies have shown that the largest source of general population exposure to DEHP is on dietary. Studies have suggested an association between exposure to phthalate plasticisers, and increased prevalence of asthma. To investigate the adjuvant effect of DEHP on asthmatic pathological changes in ovalbumin-immunised rat model. Wistar rats were randomly divided into five groups (eight rats of each group): (1) saline; (2) ovalbumin (OVA); (3) OVA'DEHP 0.7mg ×kg (1 ×d (1 ; (4) OVA'DEHP 70 mg ×kg (1 × d (1 ; and (5) DEHP 70 mg ×kg (1 ×d (1 . DEHP treatment groups which were administered to 0.7 mg and 70 mg DEHP/kg/d by gastric gavage for 30d before and during the process of OVA immunisation or saline treatment. The in vivo pulmonary function analysis, bronchoalveolar lavage (BAL) fluid collection and section histological observation were conducted. DEHP exposure could significantly increase airway hyperresponsiveness, airway remolding, and eosinophil infiltration in the OVA-immunised rats. But the DEHP exposure alone group does not show significant airway structural change and inflammatory cell infiltration, compared with control group. DEHP administered by gastric gavage play an adjuvant effect on respiratory systems in ovalbumin-immunised rat model.

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A comparative study on cockroach and ovalbumin sensitizations and challenge responses in Hartley guinea-pigs

Cited by 3 publications

References 29 publications

Back to the future: re-establishing guinea pigin vivoasthma models

Back to the future: re-establishing guinea pigin vivoasthma models

Reproducibility of a novel model of murine asthma-like pulmonary inflammation

Adjuvant effect of di-(2-ethylhexyl) phthalate on asthma-like pathological changes in ovalbumin-immunised rats

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