A comparative study on antibody immobilization strategies onto solid surface

Lee, Ji Eun; Seo, Jeong Hyun; Kim, Chang Sup; Kwon, Yunkyeoung; Ha, Jeong Hyub; Choi, Suk Soon; Joon, Hyung

doi:10.1007/s11814-013-0117-5

Cited by 38 publications

(17 citation statements)

References 13 publications

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“…A reduction in signal SPR was observed (points E to F) because of the detachment of some uncoupled not adsorbed, leaving only antibodies strongly connected. Comparing these data with previous studies by (Lee et al, 2013;Franco et al, 2013;Peluso et al, 2013), is confirmed affinity binding and irreversible adsorption of the antibody on the sensor surface, BSA is necessary to block the rest of the electrode surface, thereby preventing non-specific binding (point G). In Point I, the first positive urine sample with concentration 200μg/ml was added on the surface, where the total quantity of antigen immobilized on the electrode surface was estimated from the difference of SPR angle generated between points I and J, the value of the resonant unit (RU) measured by SPR was 496.6 millidegree.…”

Section: Spr Graphic For Detecting Antibody Positive and Negative Sasupporting

confidence: 85%

Detection of Canine Distemper Virus by Surface Plasmon Resonance

Basso

Sempionatto²,

Tozato³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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A PMMA polymer adsorbed in gold sensor through surface plasmon resonance (SPR) technique was developed for detection the canine distemper virus (CDV) through antibody/antigen binding. The antibodies anti-CDV were physically adsorbed on the surface of the sensor through covalent bonds with the thiol group formed by PMMA/EDC-NHS, the urine samples with the CDV virus were deposited on the sensor to be detected. The highest sensitive sensor surface for urine sample detection was the 200µg/ml of immobilized in 20µg/ml of antibodies, with 1 µg/ml of detection limit yielding 50 millidegree of SPR angle shift. After each binding experiment, the sensor surface was completely regenerated prior to the next measurement using 0.2 mol L −1 glycine-HCl (pH 3). All assays were performed in triplicate showing a high efficiency and good reproducibility of the technique.

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Section: Spr Graphic For Detecting Antibody Positive and Negative Sasupporting

confidence: 85%

Detection of Canine Distemper Virus by Surface Plasmon Resonance

Basso

Sempionatto²,

Tozato³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

View full text Add to dashboard Cite

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“…Consequently, the second strategy exploited the recombinant PG whose N-terminus was tailored by means of a 6-histidine chain to immobilize the Abs in a certain orientation to the Au surface. Ideally, PG binds specifically to fragment crystallizable of Abs [40], whereas fragment antigen-binding (Fab) stretches toward the liquid phase, so that Abs can maintain their natural conformation and expose more free antigen-binding regions. Thus, controlling the directional arrangement of Abs can increase the sensitivity of the biosensor.…”

Section: Antibody Immobilization Strategies On Gatementioning

confidence: 99%

Recent Advances in Immobilization Strategies for Biomolecules in Sensors Using Organic Field-Effect Transistors

Wang

Xiao

et al. 2020

Trans. Tianjin Univ.

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Organic field-effect transistors (OFETs) are fabricated using organic semiconductors (OSCs) as the active layer in the form of thin films. Due to its advantages of high sensitivity, low cost, compact integration, flexibility, and printability, OFETs have been used extensively in the sensing area. For analysis platforms, the construction of sensing layers is a key element for their efficient detection capability. The strategy used to immobilize biomolecules in these devices is especially important for ensuring that the sensing functions of the OFET are effective. Generally, analysis platforms are developed by modifying the gate/electrolyte or OSC/electrolyte interface using biomolecules, such as enzymes, antibodies, or deoxyribonucleic acid (DNA) to ensure high selectivity. To provide better or more convenient biological immobilization methods for researchers in this field and thereby improve detection sensitivity, this review summarizes recent developments in the immobilization strategies used for biological macromolecules in OFETs, including cross-linking, physical adsorption, embedding, and chemical covalent binding. The influences of biomolecules on device performance are also discussed.

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“…Rusmini et al described in depth the various schemes involved in the immobilization of protein [26]. A strong affinity towards the noble metal surfaces is exhibited by the thiol groups which allow the formation of a covalent bond between the metallic atom and the sulphur head group of thiol [27][28][29].…”

Section: Sensor Fabricationmentioning

confidence: 99%

Enhanced Biosensing Activity of Bimetallic Surface Plasmon Resonance Sensor

et al. 2019

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Surface plasmon resonance (SPR) sensors present a challenge when high sensitivity and small FWHM (full width at half maximum) are required to be achieved simultaneously. FWHM is defined by the difference between the two extreme values of the independent variable at which the value of the dependent variable is equal to half of its maximum. A smaller value of FWHM indicates better accuracy of SPR measurements. Theoretically, many authors have claimed the possibility of simultaneously achieving high sensitivity and small FWHM, which in most of the cases has been limited by experimental validation. In this report, an experimental study on the improved surface plasmon resonance (SPR) characteristics of gold over silver bimetallic sensor chips of different film thicknesses is presented. A comparative study of antigen–antibody interaction of the bimetallic chip using a custom-made, low-cost, and portable SPR device based on an angular interrogation scheme of Kretschmann configuration is performed. Pulsed direct current (DC) magnetron-sputtered bimetallic films of gold over silver were used in the construction of the SPR chip. The FWHM and sensitivity of the bimetallic sensors were firstly characterized using standard solutions of known refractive index which were later immobilized with monoclonal anti-immunoglobulin G (IgG) in the construction of the SPR biochip. Spectroscopic measurements such as ultraviolet–visible light spectroscopy (UV–Vis) and Fourier-transform infrared spectroscopy (FTIR) were used for the confirmation of the immobilization of the antibody. The performance of the bimetallic SPR biochip was investigated by exposing the sensor to various concentrations of the target protein. The results indicated that the bimetallic sensors of silver/gold had a 3.5-fold reduced FWHM compared to pure gold-based sensors, indicating a higher detection accuracy. In addition, they exhibited a significant shift in resonance angle as high as 8.5 ± 0.2 due to antigen–antibody interaction, which was ~1.42-fold higher than observed for pure silver-based sensors.

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A comparative study on antibody immobilization strategies onto solid surface

Cited by 38 publications

References 13 publications

Detection of Canine Distemper Virus by Surface Plasmon Resonance

Detection of Canine Distemper Virus by Surface Plasmon Resonance

Recent Advances in Immobilization Strategies for Biomolecules in Sensors Using Organic Field-Effect Transistors

Enhanced Biosensing Activity of Bimetallic Surface Plasmon Resonance Sensor

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