Detection of Canine Distemper Virus by Surface Plasmon Resonance

Abstract

A PMMA polymer adsorbed in gold sensor through surface plasmon resonance (SPR) technique was developed for detection the canine distemper virus (CDV) through antibody/antigen binding. The antibodies anti-CDV were physically adsorbed on the surface of the sensor through covalent bonds with the thiol group formed by PMMA/EDC-NHS, the urine samples with the CDV virus were deposited on the sensor to be detected. The highest sensitive sensor surface for urine sample detection was the 200µg/ml of immobilized in 20µg/ml of antibodies, with 1 µg/ml of detection limit yielding 50 millidegree of SPR angle shift. After each binding experiment, the sensor surface was completely regenerated prior to the next measurement using 0.2 mol L −1 glycine-HCl (pH 3). All assays were performed in triplicate showing a high efficiency and good reproducibility of the technique.

Trace detection of canine distemper virus based on surface-functionalized optic-fiber Mach–Zehnder interferometer and the Vernier effect

“…Based on the gold nanoparticle-labeled surface plasmon resonance (SPR) CDV sensor, Basso et al achieved a detection limit of 700 pg ml −1 and a detection range of 0-0.0015 ng ml −1 [11]. Basso et al fabricated the optic-fiber SPR sensor for CDV detection, the detection limit of the sensor is about 10 6 pg ml −1 in the detection range of 0.004-0.2 ng ml −1 [12]. In optic-fiber sensors, the Vernier effect is a common sensitization method, which realizes signal amplification by reducing the difference of the free spectral range (FSR) between the sensing arm and the reference arm.…”