A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods

Chen, Nai-Dong; You, Tianyan; Li, Jun; Bai, Lina; Hao, Jingwen; Xu, Xiaoyuan

doi:10.1016/j.jfda.2016.05.006

Cited by 20 publications

(27 citation statements)

References 23 publications

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“…Although some methods, such as gas chromatography–mass spectrometry fingerprint (Chen et al 2016), fourier transform infrared spectroscopy (Chen et al 2015), capillary electrophoresis (Zha et al 2009) have been applied for the authentication of Dendrobium species, they are complicated needing professional operators, cost- and time-consuming, and hard to distinguish the commercial samples that share similar textures, chemical and microscopic characteristics after processing and preparation as decoction pieces. In recent decades, molecular techniques, such as DNA barcoding (Wilson et al 2019) and real-time PCR (Sobrino-Gregorio et al 2019) have been used to differentiate many closely related species with significant advantages in the fields of taxonomy, phylogeny, evolution and breeding (Teixeira da Silva et al 2016), especially for the morphological analogues or complexes including Dendrobium species.…”

Section: Introductionmentioning

confidence: 99%

Molecular authentication and differentiation of Dendrobium species by rDNA ITS region sequence analysis

Fang

Tingmo

et al. 2019

AMB Expr

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Owing to their significant medicinal and edible values, the natural Dendrobium species have underdone over-collection and habitat destruction, and cultivated species emerged for candidates. However, these Dendrobium plants are similar in shape to be easily confused, leading to extreme difficulties for identification based on their morphological and chemical features. In this study, the rDNA ITS region sequence analysis was developed for rapid and accurate identification of thirteen wild and cultivated Dendrobium species belonging to two sections Formosae and Chrysotoxae . By cloning and sequencing the rDNA ITS region genes from 13 Dendrobium species, the phylogenetic relationships among them were analyzed. Results showed that the variation of the ITS region, together with the lengths and Guanine and Cytosine contents of ITS, 5.8s rDNA, ITS1 and ITS2 sequences occurred in the tested Dendrobium species, and which from section Chrysotoxae was higher than that from section Formsae . Phylogenetic analysis based on neighbor-joining and maximum p-arsimony trees indicated that the Dendrobium species of sections Formosae and Chrysotoxae could be well divided into two groups. A majority of Dendrobium species exhibited distinctive ITS2 secondary structures, while for those with close genetic relationships were similar. Therefore, the ITS2 region sequence analysis is simple, quick, and highly reliable that can be used as an effective tool for molecular identification and classification, as well as the reconstruction of the phylogeny of wild and cultivated Dendrobium species belonging to different sections.

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Section: Introductionmentioning

confidence: 99%

Molecular authentication and differentiation of Dendrobium species by rDNA ITS region sequence analysis

Fang

Tingmo

et al. 2019

AMB Expr

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“…Significantly different metabolic profiling between C. roseus and Vinca minor was characterized using GC-MS and LC-MS methods [ 20 ]. Recently, metabolomic characterization based on non-targeted GC-MS of medicinal Dendrobium plants was also reported [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate

Chen

Song

Zheng

et al. 2018

IJMS

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Alkaloids are the main active ingredients in the medicinal plant Dendrobium officinale. Based on the published genomic and transcriptomic data, a proposed terpenoid indole alkaloid (TIA) biosynthesis pathway may be present in D. officinale. In this study, protocorm-like bodies (PLBs) with a high-yielding production of alkaloids were obtained by the optimization of tryptophan, secologanin and methyl jasmonate (MeJA) treatment. The results showed that the total alkaloid content was 2.05 times greater than that of the control group when the PLBs were fed with 9 µM tryptophan, 6 µM secologanin and 100 µM MeJA after 36 days. HPLC analysis showed that strictosidine synthase (STR) activity also increased in the treated plants. A total of 78 metabolites were identified using gas chromatography-mass spectrometry (GC-MS) in combination with liquid chromatography-mass spectrometry (LC-MS) methods; 29 differential metabolites were identified according to the multivariate statistical analysis. Among them, carapanaubine, a kind of TIA, exhibited dramatically increased levels. In addition, a possible underlying process of the metabolic flux from related metabolism to the TIA biosynthetic pathway was enhanced. These results provide a comprehensive view of the metabolic changes related to alkaloid biosynthesis, especially TIA biosynthesis, in response to tryptophan, secologanin and MeJA treatment.

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“…Chen et al separated and relatively quantified 63 volatile compounds, with 53 being identified from three Dendrobium spp. samples by HS-GC–MS [ 62 ].…”

Section: Advanced Analytical Techniques For Quality Standard Of Tcmmentioning

confidence: 99%

Quality standard of traditional Chinese medicines: comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances

et al. 2020

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Traditional Chinese medicine (TCM) are becoming more and more popular all over the world. However, quality issues of TCM may lead to medical incidents in practice and therefore quality control is essential to TCM. In this review, the state of TCM in European Pharmacopoeia are compared with that in Chinese Pharmacopoeia, and herbal drugs that are not considered as TCM and not elaborated by TCM working party at European Directorate for the Quality of Medicines & Health Care (EDQM) but present in both European Pharmacopoeia and Chinese Pharmacopoeias are also discussed. Different aspects in quality control of TCM including origins, identification, tests and assays, as well as sample preparation, marker selection and TCM processing are covered to address the importance of establishing comprehensive quality standard of TCM. Furthermore, advanced analytical techniques for quality control and standard establishment of TCM are also reviewed. Electronic supplementary material The online version of this article (10.1186/s13020-020-00357-3) contains supplementary material, which is available to authorized users.

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A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods

Cited by 20 publications

References 23 publications

Molecular authentication and differentiation of Dendrobium species by rDNA ITS region sequence analysis

Molecular authentication and differentiation of Dendrobium species by rDNA ITS region sequence analysis

Metabolic Profiling of Dendrobium officinale in Response to Precursors and Methyl Jasmonate

Quality standard of traditional Chinese medicines: comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances

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