A Comparative Study of the Effects upon LPS Induced Macrophage RAW264.7 Inflammation <i>in vitro </i>of the Lipids of <i>Hippocampus trimaculatus</i> Leach

Abstract

The present study attempts to investigate the anti-inflammatory potential of the isolated lipid extracts of three-spot seahorse which is rare marine bony fish. Petroleum ether (PE) extract was obtained from systematic solvent extraction after reflux extraction with 95% ethanol. FrIV was collected after silica gel column chromatography, and neutral lipids (NL), glycolipids (GL), phospholipids (PL) were separated from FrIV. Basic compositions were detected and analyzed via thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). Anti-inflammatory activities of total lipids (TL), isolated NL, GL, and PL were detected by secretion of pro-inflammatory cytokines induced by lipopolysaccharide (LPS) in murine monocyte macrophage RAW264.7 cells in vitro. The results revealed that lipids of seahorse showed a positive correlation with the in vitro suppression of the release of nitric oxide (NO), interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α potently in a dose dependent manner, and showed cell compatibility. Among the fractions, GL (50 μg/mL) showed the highest capacity to attenuate the generation of pro-inflammatory cytokines which was comparable to that of the positive drug dexamethasone (DX) (20 μg/mL). Collectively, our findings indicated that the lipids from seahorse may be effective in the management of inflammation.

A Review - Biology, Aquaculture and Medical Use of Seahorse, Hippocampus spp

“…Chen [65] proved that the petroleum ether extract of seahorse was composed of phospholipids, unsaturated fatty acid and phenolic compounds, Coγ treated only), the levels of peripheral white blood cells and platelets were higher for 3 days and were significantly higher for 7 days. When it lasted for 30 days, the survival rates of experimental groups (2 groups) and control group were 33.3% (20 mg/kg), 53.3% (40 mg/kg) and 6.7% respectively [68].…”

Activation of Macrophages <i>in vitro</i> by Phospholipids from Brain of <i>Katsuwonus pelamis</i> (Skipjack Tuna)

“…It has been reported that phospholipids have anti-inflammatory action on acute exudation of inflammatory modes induced by LPS 30 , but the direct immune of PLs has barely reported. In more recent years, PL derivatives have been implicated in the regulation of immune system 31 , such as innate immune response to products of phospholipid peroxidation 32 .…”

Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages

“…aurantium [ 12 ]. In addition, the lipid extracts of Hippocampus trimaculatus , containing neutral lipids, glycolipids, phospholipids reduced the production of NO, IL-6, IL-1β, and TNF-α in LPS-stimulated RAW264.7 cells [ 18 ]. Moreover, many studies investigated the anti-inflammatory activities of various bioactive compounds from ascidian species.…”