Collagen peptides can promote wound
healing and are closely related to microbiome
colonization. We investigated the relationship among collagen peptides,
wound healing, and wound microflora colonization by administering
the murine wound model with Salmo salar skin collagen peptides (Ss-SCPs) and Tilapia nilotica skin collagen peptides (Tn-SCPs). We analyzed the vascular endothelial growth factor
(VEGF), fibroblast growth factors (β-FGF), pattern recognition
receptor (NOD2), antimicrobial peptides (β-defence14, BD14),
proinflammatory (TNF-α, IL-6, and IL-8) and anti-inflammatory
(IL-10) cytokines, macrophages, neutrophil infiltration levels, and
microbial communities in the rat wound. The healing rates of the Ss-SCP- and Tn-SCP-treated groups were
significantly accelerated, associated with decreased TNF-α,
IL-6, and IL-8 and upregulated BD14, NOD2, IL-10, VEGF, and β-FGF.
Accelerated healing in the collagen peptide group shows that the wound
microflora such as Leuconostoc, Enterococcus, and Bacillus have a positive effect on wound healing
(P < 0.01). Other microbiome species such as Stenotrophomonas, Bradyrhizobium, Sphingomonas, and Phyllobacterium had a
negative influence and decreased colonization (P <
0.01). Altogether, these studies show that collagen peptide could
upregulate wound NOD2 and BD14, which were implicated in microflora
colonization regulation in the wound tissue and promoted wound healing
by controlling the inflammatory reaction and increasing wound angiogenesis
and collagen deposition.
