A comparative study of the bispecific monoclonal antibody, blinatumomab expression in CHO cells and<i>E. coli</i>

Naddafi, Fatemeh; Shirazi, Farshad H.; Talebkhan, Yeganeh; Tabarzad, Maryam; Barkhordari, Farzaneh; Farahani, Zahra Aliabadi; Bayat, Elham; Moazzami, Reza; Mahboudi, Fereidoun; Davami, Fatemeh

doi:10.1080/10826068.2018.1525562

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Expression Systems and Approaches To Production Of Bispecifi...1

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“…Also, CHO cells ensure post-translational modifications (e.g., glycosylation) important for the functioning and stability of many recombinant proteins. Some studies have shown that the same proteins, in particular, bsAbs, produced in E. coli and CHO cells demonstrate significantly different results in the functional activity tests [55,56].…”

Section: Expression Systems and Approaches To Production Of Bispecifi...mentioning

confidence: 99%

State-of-the-Art Approaches to Heterologous Expression of Bispecific Antibodies Targeting Solid Tumors

Misorin,

Chernyshova,

Karbyshev

2023

Biochemistry Moscow

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Bispecific antibodies (bsAbs) are some of the most promising biotherapeutics due to the versatility provided by their structure and functional features. bsAbs simultaneously bind two antigens or two epitopes on the same antigen. Moreover, they are capable of directing immune effector cells to cancer cells and delivering various compounds (radionuclides, toxins, and immunologic agents) to the target cells, thus offering a broad spectrum of clinical applications. Current review is focused on the technologies used in bsAb engineering, current progress and prospects of these antibodies, and selection of various heterologous expression systems for bsAb production. We also discuss the platforms development of bsAbs for the therapy of solid tumors.

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Section: Expression Systems and Approaches To Production Of Bispecifi...mentioning

confidence: 99%