Expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems

Wang, Tianyun

doi:10.1007/s00253-020-10640-w

Cited by 24 publications

(20 citation statements)

References 157 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, expression vectors are usually integrated into the host cell genome at random and the expression level of recombinant proteins depends on the integration site on the chromosome, but most of the genomic loci are transcriptionally repressive, resulting in some transgenic sequences cannot be expressed efficiently ( Wang and Guo 2020 ). Thus, it is impossible to further increase the expression level even with optimized vectors.…”

Section: Mammalian Cells and Expression Vectorsmentioning

confidence: 99%

Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells

Wei

Mi²,

Jing

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

In recent years, mammalian cells have become the primary host cells for the production of recombinant therapeutic proteins (RTPs). Despite that the expression of RTPs in mammalian cells can be improved by directly optimizing or engineering the expression vectors, it is still influenced by the low stability and efficiency of gene integration. Transposons are mobile genetic elements that can be inserted and cleaved within the genome and can change their inserting position. The transposon vector system can be applied to establish a stable pool of cells with high efficiency in RTPs production through facilitating the integration of gene of interest into transcriptionally active sites under screening pressure. Here, the structure and optimization of transposon vector system and its application in expressing RTPs at high level in mammalian cells are reviewed.

show abstract

Section: Mammalian Cells and Expression Vectorsmentioning

confidence: 99%

Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells

Wei

Mi²,

Jing

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Most often, researchers are interested in promoters that can interact with RNA polymerase II. This enzyme is responsible for the synthesis of mRNAs, which are templates for the synthesis of polypeptides [ 1 ]. The functioning of any promoter is due to the interaction of proteins that are part of RNA polymerase with certain nucleotide motifs in the promoter.…”

Section: Structural Elements Of Dna- and Rna-based Expression Vectors...mentioning

confidence: 99%

Structural Elements of DNA and RNA Eukaryotic Expression Vectors for In Vitro and In Vivo Genome Editor Delivery

Zagoskin

Захарова²,

Nagornykh³

2022

Mol Biol

View full text Add to dashboard Cite

Gene editing with programmable nucleases opens new perspectives in important practice areas, such as healthcare and agriculture. The most challenging problem for the safe and effective therapeutic use of gene editing technologies is the proper delivery and expression of gene editors in cells and tissues of different organisms. Virus-based and nonviral systems can be used for the successful delivery of gene editors. Here we have reviewed structural elements of nonviral DNA- and RNA-based expression vectors for gene editing and delivery methods in vitro and in vivo.

show abstract

“…Even though the gene expression aspect of GECI is one of its strengths as a calcium indicator, the ramifications of longer cultivation time and phenotypic heterogeneity of transgenic cell clones can present a bottleneck to optimization of recombinant protein production in animal cell systems, as reviewed by Wang et al [143]. The background literature on bioengineering has long recognized how the components in expression vectors, including promoters, enhancers, and additional cis-elements, influence the stability and magnitude of transgene expression [144][145][146].…”

Section: Stability Of Geci Delivery In Transgenicsmentioning

confidence: 99%

Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology

Saha

2021

Biomolecules

View full text Add to dashboard Cite

Since the 1970s, the emergence and expansion of novel methods for calcium ion (Ca2+) detection have found diverse applications in vitro and in vivo across a series of model animal systems. Matched with advances in fluorescence imaging techniques, the improvements in the functional range and stability of various calcium indicators have significantly enhanced more accurate study of intracellular Ca2+ dynamics and its effects on cell signaling, growth, differentiation, and regulation. Nonetheless, the current limitations broadly presented by organic calcium dyes, genetically encoded calcium indicators, and calcium-responsive nanoparticles suggest a potential path toward more rapid optimization by taking advantage of a synthetic biology approach. This engineering-oriented discipline applies principles of modularity and standardization to redesign and interrogate endogenous biological systems. This review will elucidate how novel synthetic biology technologies constructed for eukaryotic systems can offer a promising toolkit for interfacing with calcium signaling and overcoming barriers in order to accelerate the process of Ca2+ detection optimization.

show abstract

Expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems

Cited by 24 publications

References 157 publications

Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells

Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells

Structural Elements of DNA and RNA Eukaryotic Expression Vectors for In Vitro and In Vivo Genome Editor Delivery

Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology

Contact Info

Product

Resources

About