A comparative study of the structure of tobacco mosaic virus and cucumber virus 4 by laser Raman spectroscopy

Shie, Ming‐You; Добров, Е. Н.; Tikchonenko, T.I.

doi:10.1016/0006-291x(78)91437-7

Cited by 11 publications

(9 citation statements)

References 20 publications

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“…Their spectrum of TMV RNA contains lines at 1460, 1126, 1180, 1050, 998, and 888 cm"1 which have intensities not typical of RNA (Thomas, 1970) and might be due in part to inorganic phosphate contaminants (Chapman & Thirlwell, 1964). Shie et al (1978) have also published and interpreted a…”

Section: Discussionmentioning

confidence: 99%

“…The Raman frequencies cited by Shie et al (1978) at 816 (peak) and 796 cm"1 (shoulder) are very important since they are in the region of conformationally sensitive, phosphodiester frequencies discussed above. We have reviewed all of our spectra of TMV taken both before and after the publication of the data of Shie et al (1978), at resolutions of 5 cm'1 as well as 10 cm"1, and in no case do we find a shoulder at 796 cm'1. The latter value is in fact near a minimum in our spectra.…”

Section: Discussionmentioning

confidence: 99%

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Studies of virus structure by laser Raman spectroscopy. IX. Structural studies of tobacco mosaic virus and its components by laser Raman spectroscopy

Fish¹,

Hartman²,

Stubbs³

et al. 1981

Biochemistry

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Raman spectra have been recorded for oriented gels of tobacco mosaic virus, aqueous solutions of TMV protein in the form of capsid and disk, and protein-free TMV RNA. The protein-free RNA molecule contains about 80% ordered type-A structure, which is similar to other RNA molecules in solution. RNA in the virus particle does not exhibit the base stacking and pairing normally found in protein-free RNA but instead shows at least two distinct structures characterized by different phosphodiester group frequencies in the Raman spectrum. Approximately one out of three such linkages retains the type-A geometry characteristic of aqueous RNA, while two out of three appear to have a different geometry not encountered previously for RNA. The secondary structure of the TMV coat protein molecule is similar for the three aggregation states studied (virus, capsid, and disk) and consists of 40-50% alpha helix, 40-50% irregular structure, and 0-20% beta sheet. Two of the three tryptophan residues per protein molecule reside in hydrophobic regions, and the third is in contact with water. The distribution of the four tyrosine residues per protein molecule among hydrogen-bonding states is the same for capsid and virus but different for the disk. The latter could be a consequence of the presence of Cl- ions which may form hydrogen bonds with one or more p-hydroxyl groups of tyrosine residues.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Studies of virus structure by laser Raman spectroscopy. IX. Structural studies of tobacco mosaic virus and its components by laser Raman spectroscopy

Fish¹,

Hartman²,

Stubbs³

et al. 1981

Biochemistry

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“…Therefore, the enhancement factor can be corrected to be at least 10 6 , which corresponds well with values given in the literature. [19,28,29] However, an even higher enhancement factor can be achieved by adjusting the excitation wavelength to perfectly match with the [33] 1629 1632 1632 Tyr, Phe, Trp [9,32] 1617…”

Section: Resultsmentioning

confidence: 99%

“…Trp [12,30,32,33] 1331 1338 1337 1333 C-H def in proteins, Trp [12,31 -33] ; A, G [10,12,31,32] 1298 1301 C, A, U [10,12,32,33] ; Amide III [12,30] 1186 1187 1191 1195 Tyr, Phe [32,33] 1134 C-N stretch in proteins [12,30,32,33] ; C, U [32] 1035 1045…”

Section: Resultsmentioning

confidence: 99%

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Raman to the limit: tip‐enhanced Raman spectroscopic investigations of a single tobacco mosaic virus

Cialla

Deckert‐Gaudig

Budich

et al. 2008

J Raman Spectroscopy

167

142

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The development of fast identification techniques of viruses is an ongoing important research topic. Conventional virus detection and identification is generally based on various different microbiological methods. However, these techniques are not suitable for the analysis of single virus particles. Therefore, our goal is to establish tip-enhanced Raman scattering (TERS), providing vibrational spectroscopic information with a spatial resolution less than 50 nm, to characterize single viruses at a molecular level. Here we report, to the best of our knowledge for the first time, about TERS spectra of a tobacco mosaic virus, showing the great capability of this technique. However, the application of the TERS technique for a rapid and direct detection of different species of single viruses is under development, which is useful for a wide range of analytical fields.

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References

1995

Infrared and Raman Spectroscopy

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A comparative study of the structure of tobacco mosaic virus and cucumber virus 4 by laser Raman spectroscopy

Cited by 11 publications

References 20 publications

Studies of virus structure by laser Raman spectroscopy. IX. Structural studies of tobacco mosaic virus and its components by laser Raman spectroscopy

Studies of virus structure by laser Raman spectroscopy. IX. Structural studies of tobacco mosaic virus and its components by laser Raman spectroscopy

Raman to the limit: tip‐enhanced Raman spectroscopic investigations of a single tobacco mosaic virus

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