A comparative study of some physico-chemical properties of human serum albumin samples from different sources—I

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“…On the other hand, Kane (7) found no influence of the batches of amino acids. Droge et al (6) have shown that the main differences between albumin lots concerned their fatty acid content, which have a great influence on embryo development. Indeed, palmitic acid and polyunsaturated fatty acids inhibit mouse embryo development through the generation of superoxide radicals (30).…”

“…The use of such supplementation raises two problems: reproducibility and safety. Indeed, its has been shown that some sera could have detrimental effects on embryo growth (4,5) and that there are wide batch-tobatch variations among albumin preparations (6,7). To solve this problem, standardized serum substitutes, which contain proteins extracted from pooled human sera (8), have been successfully used in in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) (9,10).…”

Baboon serum is superior to human or bovine serum albumin for baboon sperm capacitation and zona binding

“…Indeed, replacement of BSA with polyvinylalcohol (without albumin) resulted in decreased rhesus macaque sperm–zona binding with complete absence of zona penetration [22]. Variability in albumin contents and in fatty acid composition among serum batches may partly explain the variable efficiency of culture media in sperm capacitation and IVF [8, 11, 14]. Furthermore, variation in protein source results in major variations in the zona‐free hamster egg penetration test [6].…”

The fatty-acid-induced conformational states of human serum albumin investigated by means of multiple co-binding of protons and oleic acid

“…820512. The albumin was deionized before use and its concentration determined as described elsewhere (Droge et al, 1982). The deionizing procedure yielded a protein solution in which 0.9 mol of fatty acid is bound/mol of albumin.…”

“…The method of measurement has been described previously (Dr6ge et al, 1982). We used this albumin sample with 0.9 mol of fatty acid/mol of albumin as our starting material because we thought it might be advisable to work with albumin that contains some bound fatty acid (Droge et al, 1982). This value of 0.9 is included in those quoted in the present paper and also in the Figures shown.…”