“…The use of such supplementation raises two problems: reproducibility and safety. Indeed, its has been shown that some sera could have detrimental effects on embryo growth (4,5) and that there are wide batch-tobatch variations among albumin preparations (6,7). To solve this problem, standardized serum substitutes, which contain proteins extracted from pooled human sera (8), have been successfully used in in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) (9,10).…”