Baboon serum is superior to human or bovine serum albumin for baboon sperm capacitation and zona binding

Abstract

Baboon serum is superior to HSA or BSA for baboon sperm capacitation and zona binding.

Assisted Reproductive Technologies (ART) With Baboons Generate Live Offspring: A Nonhuman Primate Model for ART and Reproductive Sciences

“…Certainly, optimizing in vitro culture media will be paramount in improving preimplantation development in the baboon, as shown for other NHP species (Wolf, 2004). Interestingly baboon serum is superior to human serum or bovine serum albumin for inducing capacitation in baboon sperm (Nyachieo et al, 2009a; Nyachieo et al, 2009c). …”

“…Protocols for baboon ART are now generating live offspring by ART and further improvements can be anticipated by this and other teams (e.g. Nyachieo et al, 2009a; (Nyachieo et al, 2009b). Yet, one reason why NHPs are not fully contributing to bridging the intellectual and biomedical gaps between rodents and people is because of continuing and significant limitations in NHP research resources.…”

“…We investigated 9 protocols for the hyperstimulation of female baboons (Table 1), starting on Days 1-2 from the onset of visible menses (Nyachieo et al, 2009a; Nyachieo et al, 2009b; Nyachieo et al, 2009c). Protocol I : a single daily intr-amuscular injection of 60 IU recombinant human follicle stimulating hormone (r-FSH; Gonal-F; Serono, Randolph, MA) for 9 days, followed by a final subcutaneous injection of 1000 IU of recombinant human chorionic gonadotropin (r-hCG; Ovidrel; Serono); Protocol II : a daily intra-muscular injection of 60 IU r-FSH and daily subcutaneous injection of 0.25 μg/ml of a gonadotropin-releasing hormone (GnRH) agonist (Leuprolide Acetate; Sicor Pharmaceuticals, Irvine, CA) for 9 days, the final 3 of which also included 60 IU recombinant human luteinizing hormone (r-hLH; Luveris; Serono) combined with the r-FSH.…”

“…Assisted Reproductive Technologies (ART) using nonhuman primates (NHPs) have been instrumental in bridging gaps between research in rodents, nearly exclusively using mice, and humans (reviewed by ((Hewitson, 2004); (Nyachieo et al, 2009a 2009c; Nyachieo et al, 2009b 2009c; Nyachieo et al, 2009c 2009c). Now thirty years after the birth of the first child conceived by ART (Edwards et al; Steptoe and Edwards; Steptoe and Edwards; Steptoe et al), clinical ART practices raise significant biological and medical issues – many of which can best be solved by responsible investigations of NHP ART outcomes.…”

“…Because research advances by confirming results in related species, and also because variations exist between related species, we have sought to expand the baboon as an alternative research resource to macaques (review by (Nyachieo et al, 2009a; Nyachieo et al, 2009b; Nyachieo et al, 2009c)). Previously, Nyachieo et al (2009) had shown that the baboon menstrual cycle could be synchronized using oral contraceptives followed by successful ovarian stimulation using a GnRH agonist in combination with human recombinant hormones. The authors showed an average yield of 19 oocytes at retrievals, with an ICSI fertilization rate of 23-54% and successful development to the 8-cell stage.…”