2001
DOI: 10.1590/s0074-02762001000400022
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A comparative study of preservation and storage of Haemophilus influenzae

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Cited by 27 publications
(13 citation statements)
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“…Isolates were identified using standard biochemical tests such as the oxidase test, growth and lactose fermentation in MacConkey and triple sugar iron agar, the MRVP test, and growth in Simmons' citrate agar (9). All isolates were stored at -80°C in tryptic soy broth containing 40% glycerol (10).…”
Section: Bacterial Isolatesmentioning
confidence: 99%
“…Isolates were identified using standard biochemical tests such as the oxidase test, growth and lactose fermentation in MacConkey and triple sugar iron agar, the MRVP test, and growth in Simmons' citrate agar (9). All isolates were stored at -80°C in tryptic soy broth containing 40% glycerol (10).…”
Section: Bacterial Isolatesmentioning
confidence: 99%
“…Alternatively, skim milk, a common freeze-drying protectant (Barbaree et al, 1982), can be used as a freezing solution (Takahashi et al, 1982, Essiain & Flournoy, 1986, Sinha et al, 1974, Hasegawa et al, 1967, as is regularly used in our laboratory to store Pseudomonas aeruginosa. A literature review indicates that the predominant studies of cyroprotectants focus on sensitivity to freeze/thaw cycles (Takahashi et al, 1982, Aulet de Saab et al, 2001 with few comparisons of viability over time at elevated temperature (Gruft et al, 1968, Essiain & Flournoy, 1986. While recovering our bacterial strains from 31 days at ambient temperatures (in this case, sustained temperatures of ∼30°C) due to power loss as a result of hurricanes Katrina and Rita, we observed an increase in recovery of strains that were stored in 10% skim milk versus 15% glycerol.…”
Section: Introductionmentioning
confidence: 72%
“…Isolates were cultured on Haemophilus test medium, confi rmed as H. infl uenzae using standard methodology and stored as glycerol stock until further use. [8,9] capB gene of H. infl uenzae is known as virulence gene as Type b capsular polysaccharide is a major virulence factor and target for serum antibodies. Isolates with Type b serotype from both invasive and commensal isolates were detected through capB polymerase chain reaction (PCR) as described earlier.…”
Section: Methodsmentioning
confidence: 99%