“…Originally, PBP and GOBP in adult moths were considered to be absent from the thorax, midgut, fat body, and metabolic tissues but abundant in the antennae and legs; this also included proteins/genes of the current study, namely, “PBP1, PBP2, GOBP1, and GOBP2” of the silkworm moth, B. mori (BmorPBP1, BmorPBP2, BmorGOBP1, and BmorGOBP2; Vogt et al, 1991a , b ; Krieger et al, 1996 ; Sandler et al, 2000 ; Forstner et al, 2006 ; Gong D. et al, 2009 ; Zhou et al, 2009 ; Li et al, 2012 ; Xuan et al, 2014 ; Picimbon, 2019 ). In addition, while the larval expression of GOBP2 was restricted to large sensilla basiconica, sensilla styloconica, or other gustatory chemosensilla from maxillary palps and antenna (Vogt et al, 2002 ), the adult expression of BmorGOBP2 was shown to be associated with the female moth pheromone gland (Xuan et al, 2014 ), strongly suggesting multiple functions for this protein. Gel digestion of SDS-PAGE-separated proteins and liquid chromatography coupled to tandem mass spectrometry (Nano-LC/MS/MS) analysis showed the presence of GOBP2 with many other OBPs (OBP6, OBP56d, PBP-related protein 3, sericotropin, and protein B1) in a library of more than 9,000 peptides from the Bombyx pheromone gland (Xuan et al, 2014 ), urging us to use molecular biology and quantitative real-time PCR as an alternative/complementary approach of biochemistry and immunoblotting to focus on the PBP/GOBP clade.…”