A comparative study of novel spectrophotometric methods based on isosbestic points; application on a pharmaceutical ternary mixture

Lotfy, Hayam M.; Saleh, Sarah S.; Hassan, Nagiba Y.; Salem, Hesham

doi:10.1016/j.saa.2014.01.130

Cited by 56 publications

(36 citation statements)

References 25 publications

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“…This method was based on the same principle as the absorption factor method [34][35][36][37]. The method has mainly depended on the presence of an isoabsorptive point in the zero order absorption spectra of the drugs (λiso 267 nm) where the drugs have equal absorptivities at this point.…”

Section: Absorbance Subtraction Methods (As)mentioning

confidence: 99%

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Simultaneous determination of levonorgestrel and ethinyl estradiol in combined dosage form utilizing spectrophotometric methods and high performance thin layer chromatographic method on nanosilica gel plates

et al. 2017

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Simultaneous quantification of levonorgestrel (LEV) and ethinyl estradiol (EE) was performed utilizing five different spectrophotometric methods and a high performance thin layer chromatographic method (HPTLC). The applied spectrophotometric methods were based on either ratio spectra namely; ratio difference, ratio subtraction and derivative ratio or the presence of isosbestic point specifically; absorbance subtraction and amplitude modulation. The proposed methods had the ability to resolve the overlapped spectra of the drugs with a linear relationship in the concentration range 1-65 µg/mL and 1-95 µg/mL for LEV and EE, respectively. The developed HPTLC method has revealed a good separation of the drugs upon utilizing Nano Silica Gel on TLC plates with fluorescent indicator 254 nm glass plates as the stationary phase and chloroform: methanol (99:1, v:v) as the mobile phase. The proposed HPTLC method has shown high sensitivity, where the linearity range was 0.02-3.00 µg/band and 0.5-20.0 µg/band, for LEV and EE, respectively. The proposed methods were successfully applied for the analysis of laboratory prepared mixtures as well as combined dosage form. Validation for all methods was conducted in compliance with the ICH guidelines proving the methods to be selective, linear, precise and accurate. The proposed methods were statistically compared with the pharmacopoeial method, where the obtained results showed no significant difference regarding accuracy and precision.

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Section: Absorbance Subtraction Methods (As)mentioning

confidence: 99%

“…In addition, the extension of the spectra of one component was another requirement [34,36]. The AM method has depended on the utilization of the normalized divisor to eliminate the effect of the choice of the divisor which greatly affects the results of manipulating ratio spectra techniques.…”

Section: Amplitude Modulation Methods (Am)mentioning

confidence: 99%

Simultaneous determination of levonorgestrel and ethinyl estradiol in combined dosage form utilizing spectrophotometric methods and high performance thin layer chromatographic method on nanosilica gel plates

et al. 2017

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“…The concentration of each X and Y, separately, is calculated using the isobsorptive point unified regression equation (obtained by plotting the absorbance values of the zero order spectra of either X or Y at isobsorptive point (k iso ) against the corresponding concentrations X or Y respectively) [41][42][43].…”

Section: Absorbance Subtraction Methods (As)mentioning

confidence: 99%

A comparative study of the novel spectrophotometric methods versus conventional ones for the simultaneous determination of Esomeprazole magnesium trihydrate and Naproxen in their binary mixture

Lotfy

Amer

Zaazaa

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…Ternary mixtures suffer a higher degree of difficulty in spectrophotometric resolution than binary mixtures, due to the severe overlap usually noticed in their spectra. Many methods have been developed for resolution of the overlapped spectra of ternary mixtures using different spectrophotometric and chemometric techniques [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Mean centering of double divisor ratio spectra, a novel spectrophotometric method for analysis of ternary mixtures

Hassan

Elzanfaly

Salem

et al. 2016

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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A comparative study of novel spectrophotometric methods based on isosbestic points; application on a pharmaceutical ternary mixture

Cited by 56 publications

References 25 publications

Simultaneous determination of levonorgestrel and ethinyl estradiol in combined dosage form utilizing spectrophotometric methods and high performance thin layer chromatographic method on nanosilica gel plates

Simultaneous determination of levonorgestrel and ethinyl estradiol in combined dosage form utilizing spectrophotometric methods and high performance thin layer chromatographic method on nanosilica gel plates

A comparative study of the novel spectrophotometric methods versus conventional ones for the simultaneous determination of Esomeprazole magnesium trihydrate and Naproxen in their binary mixture

Mean centering of double divisor ratio spectra, a novel spectrophotometric method for analysis of ternary mixtures

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