A comparative study of microbial dynamics and phosphorus removal for a two side-stream wastewater treatment processes

Zhang, Yanyan; Islam, Md. Shahinoor; McPhedran, Kerry N.; Dong, Shimiao; Rashed, Ehab M.; Elshafei, Mohamed; Noureldin, Ahmed M.; El-Din, Mohamed Gamal

doi:10.1039/c7ra07610j

Cited by 18 publications

(6 citation statements)

References 55 publications

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“…Acinetobacter-0.2-2.4% in R1, 0.3-2.4% in R2, and 0.0-2.6% in R3-persistent but usually low in abundance in AGS reactors [46], were also found. Environmental conditions control the abundance of each bacterial species [47].…”

Section: Distribution Of the Microbial Profilementioning

confidence: 99%

Phosphorus Removal from Aerobic Granular Sludge: Proliferation of Polyphosphate-Accumulating Organisms (PAOs) under Different Feeding Strategies

et al. 2022

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Aerobic granular sludge (AGS) is known for high phosphorus removal from wastewaters, and phosphorus can be recovered from high phosphorus-containing waste sludge granules. This study aimed at determining the feeding strategy that provides the best performance in terms of the proliferation of polyphosphate-accumulating organisms (PAOs) and phosphorus removal. Using three AGS bioreactors, this study compared phosphorus removal and the proliferation dynamics of PAOs under three different feeding strategies: anaerobic slow feeding (R1), pulse feeding + anaerobic mixing (R2), and pulse feeding (R3). Results indicate that R1 and R2 achieved significantly higher phosphorus removal (97.6 ± 3% for R1 and 98.3 ± 1% for R2) than R3 (55 ± 11%). The anaerobic slow feeding procedure (R1) achieved the highest specific phosphorus release rate (SPRR) and specific phosphorus uptake rate (SPUR) as compared to the other two feeding conditions. 16S ribosomal ribonucleic acid (rRNA) gene sequencing assay of the microbial community for the three feeding strategies indicated that although the feeding strategy impacted reactor performance, it did not significantly alter the microbial community. The bacteria community composition maintained a similar degree of diversity. Proteobacteria, Bacteroidetes, and Verrucomicrobia were the dominant bacterial phyla in the system. Dominant PAOs were from the class Betaproteobacteria and the genera Paracoccus and Thauera. Glycogen-accumulating organisms were significantly inhibited while other less-known bacteria such as Wandonia and Hyphomonas were observed in all three reactors.

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Section: Distribution Of the Microbial Profilementioning

confidence: 99%

Phosphorus Removal from Aerobic Granular Sludge: Proliferation of Polyphosphate-Accumulating Organisms (PAOs) under Different Feeding Strategies

et al. 2022

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“…Dechloromonas phosphorivorans abundances ranging from 0.1 to 1.9% in EBPR facilities and observed up to 1% relative abundance in non-EBPR facilities. Prior to that study and the proposal of the Dechloromonas PAO taxonomic classification, unclassified Dechloromonas were enriched in a variety of lab-scale and full-scale EBPR bioreactors. ,− To our knowledge, no other full-scale studies have identified Dechloromonas, specifically Ca . Dechloromonas phosphorivorans, as a key PAO in S2EBPR configurations.…”

Section: Resultsmentioning

confidence: 99%

Confronting Assumptions of Phosphorus-Accumulating Organisms and Glycogen-Accumulating Organisms: Peaceful Coexistence in a Carbon-Limited Sidestream EBPR Demonstration

Farmer

Sabba²,

Jia

et al. 2023

ACS EST Water

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Enhanced biological phosphorus removal (EBPR) is strongly influenced by the influent ratio of readily biodegradable carbon to soluble phosphorus due to the preferences of phosphorus-accumulating organisms (PAO). The sidestream EBPR (S2EBPR) process redirects a portion of return activated sludge (RAS) to a sidestream fermenter, increasing the availability of biodegradable carbon. In this study, we assessed the performance and microbial community structure of a full-scale S2EBPR demonstration supplemented with external carbon dosing. By the end of the 10 month study period, the demonstration achieved a median effluent orthophosphate of 0.3 mg/L. The microbial community consisted of a common core microbiome in the RAS fermenter, EBPR basin, and nitrification basin. The most abundant PAO detected were Ca. “Dechloromonas phosphorivorans”, while the canonical PAO Ca. “Accumulibacter” and Tetrasphaera were observed in lower relative abundance. In addition to non-canonical PAO enrichment, the glycogen-accumulating organism Ca. “Competibacter” proliferated throughout the study and at some points outnumbered PAO taxa by 30 to 1 with no tangible performance impacts. This study provides insights into successful S2EBPR implementation at a low-carbon facility and improves our understanding of microbial community structure and key PAO and GAO in S2EBPR systems.

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“…The presence of GAOs might interfere with the accumulation of poly-P by PAOs. 11 The PHA compounds contained PHB and PHV, and their values increased with the release proceeding. The increased PHA content accelerated the disintegration and the following cell lysis of anaerobic sludge.…”

Section: Intracellular Phosphorus Contentsmentioning

confidence: 95%

“…In addition, anaerobic sludge is the pool of microorganisms for P release, which relies on a group of bacteria, i.e., phosphorus accumulating organisms (PAOs). 11 PAOs could store carbon sources as intracellular polymeric (poly-b-hydroxyalkanoate, PHA) and release ortho-P from the microbial cells using the energy of the degradation of glycogen and polyphosphate (poly-P). [12][13][14] PAOs are identied as mesophiles or even psychrophiles.…”

Section: Introductionmentioning

confidence: 99%

Temperature effect on extracellular polymeric substances (EPS) and phosphorus accumulating organisms (PAOs) for phosphorus release of anaerobic sludge

2019

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A comparative study of microbial dynamics and phosphorus removal for a two side-stream wastewater treatment processes

Abstract: A side-stream process with anoxic/aerobic tanks was designed as a denitrifying enhanced biological phosphorus removal process for wastewater treatment as compared to a modified side-stream process using contact/stabilization tanks.

Cited by 18 publications

References 55 publications

Phosphorus Removal from Aerobic Granular Sludge: Proliferation of Polyphosphate-Accumulating Organisms (PAOs) under Different Feeding Strategies

Phosphorus Removal from Aerobic Granular Sludge: Proliferation of Polyphosphate-Accumulating Organisms (PAOs) under Different Feeding Strategies

Confronting Assumptions of Phosphorus-Accumulating Organisms and Glycogen-Accumulating Organisms: Peaceful Coexistence in a Carbon-Limited Sidestream EBPR Demonstration

Temperature effect on extracellular polymeric substances (EPS) and phosphorus accumulating organisms (PAOs) for phosphorus release of anaerobic sludge

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