Temperature effect on extracellular polymeric substances (EPS) and phosphorus accumulating organisms (PAOs) for phosphorus release of anaerobic sludge

Zeng, Fanzhe; Jin, Wei; Zhao, Qingliang

doi:10.1039/c8ra10048a

Cited by 23 publications

(11 citation statements)

References 51 publications

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“…For example, P-PO 4 3− in EPS was found to be just 5 mg/gVSS in one study, which was much lower than that of the WAS cell content (∼60 mg/gVSS). 12 Thus, dosing with ADC is a logical method to recover CH 4 or VFAs with low associated N and P release, and this is further demonstrated below.…”

Section: Was Fermentation By Dosing With Adcmentioning

confidence: 95%

“…11 N (such as protein and amino sugar) and P (such as DNA and cytomembrane) are known to be mainly stored within the microbial cell, although these nutrients are rather low in EPS. 12 For example, Liu et al reported that the released protein increased notably from 325 to 988 mg/L after dosing lysozyme in WAS. 13 Soluble DNA also increased from 7 to 34 mg/L by the cation exchange resin (CER)-induced hydrolysis of WAS.…”

Section: ■ Introductionmentioning

confidence: 99%

“…reported that a pH of 8.9 provided optimized conditions for the maximum acidification of WAS with an associated VFA production of 423 mgCOD/gVSS (where VSS was the volatile suspended solid) . N (such as protein and amino sugar) and P (such as DNA and cytomembrane) are known to be mainly stored within the microbial cell, although these nutrients are rather low in EPS . For example, Liu et al reported that the released protein increased notably from 325 to 988 mg/L after dosing lysozyme in WAS .…”

Section: Introductionmentioning

confidence: 99%

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Highly Selective Fermentation of Waste-Activated Sludge by Alginate-Degrading Consortia

et al. 2021

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Produced waste-activated sludge (WAS) can be recovered as a promising carbon source in wastewater treatment plants. However, the cell integrity is always ignored, which results in the unwanted release of N and P. In this work, an alginate-degrading microbial consortium (ADC) with a higher percentage of genus Bacteroides (>90%) was enriched. The role of enriched ADC on the productions of methane and volatile fatty acids (VFAs) and cell integrity (via lactate dehydrogenase (LDH) activity and DNA concentrations) was then investigated in WAS fermentation. The results showed that dosing with ADC increased methane production by 53% without P release. Methane production of 440 mL from extracted extracellular polymeric substances was comparable to that from WAS (445 mL). After inhibiting methanogenesis, VFA concentrations via dosing with ADC were comparable to those of alkali fermentation, while expectantly showing lower N (114 vs 367 mg/L) and P (0 vs 122 mg/L) release. Since N and P are mainly stored within cells, such better selectivity was attributed to the ADC not affecting cell integrity, which was also verified by LDH activity and DNA concentrations. Consequently, these results provide a highly selective microbial method to produce biochemicals from WAS while maintaining cell integrity.

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Section: Was Fermentation By Dosing With Adcmentioning

confidence: 95%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Highly Selective Fermentation of Waste-Activated Sludge by Alginate-Degrading Consortia

et al. 2021

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“…As a major part of sludge, extracellular polymeric substances (EPS) play a significant role in phosphorus removal through preserving orthophosphate, pyrophosphate, and polyphosphate. − Furthermore, some phosphate precipitates adsorbed or formed in situ inside sludge might also be associated with EPS . Phosphate minerals, such as particles rich in magnesium and phosphorus or calcium and phosphorus, were detected in EPS extracted from various sludge systems. , In addition, struvite was also observed in the isolated sludge EPS after the reaction with ammonium, although this qualitative characterization could not confirm that the struvite formation was due to induced precipitation by EPS, as struvite could also be present in the original EPS.…”

Section: Introductionmentioning

confidence: 99%

Observing the Biologically Induced Phosphate Precipitation by Sludge Extracellular Polymeric Substances in Enhanced Biological Phosphorus Removal

et al. 2022

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Biologically induced phosphate precipitation is a common phenomenon in the enhanced biological phosphorus removal (EBPR) process. In this study, as observed by backscattered scanning electron microscopy coupling energy-dispersive X-ray spectroscopy, large amounts of phosphate precipitates (mainly Ca phosphate) were found to be accumulated in EBPR granular sludge. The Ca phosphate mineral inside EBPR granules was present in two forms: independent mineral clusters and aggregates along with cells packaged by sludge extracellular polymeric substances (EPS). In addition, speciation of phosphorus in EPS extracts was investigated. It was found that about 20.7 ± 2.4% of the EPS-associated phosphorus was found present in a nanosized particulate form by single particle-inductively coupled plasma-mass spectroscopy, and phosphate precipitation induced by isolated EPS was confirmed. Therefore, it was proposed that EPS might play an important role in promoting the phosphate precipitation process through adsorption or entrapment of precipitates to EPS or growth of precipitates inside EPS. Results in this study will be helpful to better understand the phosphorus removal approach through induced precipitation in granular EBPR systems.

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“…Acetate and propionate are the typical volatile fatty acids (VFAs) in sewage or municipal wastewater, which are key carbon sources for the EBPR process [19,20]. Temperatures higher than 20 °C were generally reported as unfavorable to the EBPR process, so the EBPR technique might not be suitable for tropical climates with temperatures above 30 °C [21,22,23,24]. Furthermore, the content and composition of LB-EPS and TB-EPS as well as their roles in biological phosphorus removal might also be influenced by the type of organic substrate and the temperature.…”

Section: Introductionmentioning

confidence: 99%

Layered Extraction and Adsorption Performance of Extracellular Polymeric Substances from Activated Sludge in the Enhanced Biological Phosphorus Removal Process

Xi²

2019

Molecules

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A large amount of phosphorus was found in the extracellular polymeric substances (EPS) of activated sludge used in enhanced biological phosphorus removal (EBPR), so the role of EPS and extracellular phosphorus in EBPR should not be neglected. The composition and properties of tightly bound EPS (TB-EPS) and loosely bound EPS (LB-EPS) were significantly different, and it was necessary to study the adsorption performance of EPS through the fractionating of activated sludge into LB-EPS, TB-EPS and microbial cells. In this study, the adsorption performance of LB-EPS and TB-EPS for phosphate was explored by extracting LB-EPS and TB-EPS via sonication and cation exchange resin (CER), respectively. The results indicated that the sonication-CER method was an efficient and reliable extraction method for EPS with a synergistic effect. The performance of EPS in the adsorption/complexing of phosphate was excellent because of its abundant functional groups. Specifically, the type and content of metal elements and functional groups in TB-EPS were much greater than those in LB-EPS, which led to the key role of TB-EPS in the adsorption/complexing of phosphate. Finally, a metabolic model for EBPR with consideration of the adsorption performance of LB-EPS and TB-EPS was proposed.

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Temperature effect on extracellular polymeric substances (EPS) and phosphorus accumulating organisms (PAOs) for phosphorus release of anaerobic sludge

Abstract: Phosphorus (P) is an essential element for living organisms and anaerobic sludge is an attractive source for P recovery.

Cited by 23 publications

References 51 publications

Highly Selective Fermentation of Waste-Activated Sludge by Alginate-Degrading Consortia

Highly Selective Fermentation of Waste-Activated Sludge by Alginate-Degrading Consortia

Observing the Biologically Induced Phosphate Precipitation by Sludge Extracellular Polymeric Substances in Enhanced Biological Phosphorus Removal

Layered Extraction and Adsorption Performance of Extracellular Polymeric Substances from Activated Sludge in the Enhanced Biological Phosphorus Removal Process

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