A Comparative Study of in Vitro Antimicrobial Activity and TLC Studies of Petals of Selected Indian Medicinal Plants

Abstract

Objective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.

Characterisation of Chamaecytisus tagasaste, Moringa oleifera and Vachellia karroo Vermicomposts and Their Potential to Improve Soil Fertility

“…Alkaloids were not detected in M. oleifera and V. karroo but only in C. tagasaste. These results are in contrast with Rengarajan, Melanathuru [68] who found that M. oleifera contained alkaloids among other phytochemicals. It is not immediately clear why none of the analysed phytochemicals were not detected in V. karroo vermicompost in this study.…”

“…These results are also in agreement with Rengarajan, Melanathuru [68] who noted that M. oleifera contains flavonoids among other phytochemicals. Legumes exude specific flavonoids that act as signalling molecules to attract N-fixing bacteria [78], which is essential in making N available in the soil for plant use.…”

“…Moringa oleifera is known to be rich in different forms of phytochemicals [67], which could have been the reason for the presence of tannins as well as flavonoids observed in this study. This is also supported by Rengarajan, Melanathuru [68] who indicated that methanol extracts of M. oleifera petals contained flavonoids, tannins, saponins and alkaloids. Kraus, Yu [69] reported that tannins may limit litter decomposition in a number of different ways such as by themselves being resistant to decomposition or by sequestering proteins in protein-tannin complexes that are resistant to decomposition.…”

Anthocyanin Profile and Its Antioxidant Activity of Widely Used Fruits, Vegetables, and Flowers in Thailand

“…Similarly, Turkyılmaz (2013) reported cyanidin-3,5-diglucoside as major anthocyanins present in the PJs of Turkey varieties and PJ also contains cyanidin-3-glucoside, delphinidin-3-glucoside, pelargonidin-3-glucoside, delphinidin-3,5-diglucoside, and pelargonidin-3,5-diglucoside [53]. The content of anthocyanin and antioxidant property of methanolic extract of Po has already been reported [54]. Borochov-Neori et al (2011) reported the effect of climate on the composition of anthocyanin and its content Po fruit arils [55].…”