A Comparative Study of Immunohistochemical Expression of Moesin , Cytokeratin 14 and MMP7 in Oral Squamous Cell Carcinoma and Oral Verrucous Carcinoma

Abstract

Background: Squamous cell carcinoma (SCC) is the most prevalent malignant neoplasm of the oral cavity that exhibits certain histological variations. Verrucous carcinoma (VC) is an uncommon exophytic low-grade welldifferentiated variant of SCC. Cellular differentiation and morphology play important roles in cell functions and maintenance of structural integrity .As the cancer is a malignant process in which disorder of the cell growth and behavior occurs, such changes may differ in different tumor types and within different grades of the same tumor. Materials and Methods:Forty two formalin-fixed, paraffin-embedded tissue blocks were included in this study (30 blocks were diagnosed as OSCC and 12 blocks were diagnosed as OVC). An immunohistochemical staining was performed using anti moesin, anti CK14 and anti MMP7 monoclonal antibodies. Results: Moesinimmunoreactivity was recognized in all the studied groups with predominant cytoplasmic expression in OSCC & membranous expression in OVC. No difference was noticed between 2 studied groups &between different grades of OSCC. Cytokeratin 14 positivity was noticed in all studied groups with significant difference between OSCC&OVC (p=0.012) & there was a significant difference between the different grade of OSCC (p=0.047). MMP7 expression was observed in the all studied groups with predominant cytoplasmic pattern in OSCC & nuclear pattern in OVC. No difference was found between the 2studied groups &between the different grades of SCC. A Strong positive linear correlation between MMP7&CK14 was noticed. Conclusion: Verrucous carcinoma has a specific pattern for moesin and MMP7 that differs from OSCC, however; the difference is not significant. CK14 immunoreactivity indicated a significant difference in the degree of cellular differentiation between OSCC &OVC.

To evaluate an association between prognostic significance of Moesin with histopathological grading of oral squamous cell carcinoma: A systematic review

“…Most studies in this review found that Moesin was strongly associated with the histopathological grading for cancer[ 6 8 9 16 18 26 ] but was not elicited in a study[ 17 ] done in Baghdad where no statistical significance was elicited between the expression of Moesin and its association with the histopathological grading in OSCC cases. In a study[ 26 ] on laryngeal squamous cell carcinoma (LSCC), Moesin expression did not show any association with the advanced clinical staging (III and IV).…”

“…Most of the studies were cross-sectional and were conducted in university hospitals, cancer hospitals, or tertiary care centers. [ 6 9 16 17 18 20 27 ] The tissues samples were stereotypically collected from male patients, more than 55 years of age, and at least 40% of the samples showed marked expression of Moesin in their cytoplasm [ Table 1 ].…”