A comparative study of ELISA and other methods for the detection of Brucella antibodies in bovine sera

Aert, A. Van; Brioen, Paul; Dekeyser, P.; Uytterhaegen, L; Sijens, Robert; Boeyé, Albert

doi:10.1016/0378-1135(84)90052-x

Cited by 26 publications

(14 citation statements)

References 9 publications

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“…This may be due to selection techniques, such as the use of pigs 2 to 3 months of age, which are susceptible to infection with B. suis but have very limited ability to produce antibody (16). There may also be a lack of antibody response in the very early stages of infection, or seronegative animals may have been infected without exhibiting any serological or clinical evidence of infection (21). The actual sensitivity values were close, due to the small sample set, and predicting which test would be more accurate would be difficult.…”

Section: Discussionmentioning

confidence: 99%

“…The 2-ME test is not included in the Office International des Epizooties manual. A general problem with all conventional tests and some primary binding assays is that they detect antibody resulting from exposure to cross-reacting organisms, such as Y. enterocolitica O:9, a problem in some areas (4,21). This is demonstrated by data found at the Statens Veterinaere Serumlaboratorium, Copenhagen, Denmark, website (http:// www.svs.dk/dk/ye-rapp.htm) showing that the complement fixation test does not distinguish between antibodies produced by Y. enterocolitica O:9 and antibodies produced by B. suis or B. abortus in 70% of the sera tested while the CELISA was positive in only 14% of the cases.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina

Paulo

Vigliocco

Ramondino

et al. 2000

Clin Diagn Lab Immunol

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An indirect enzyme-linked immunosorbent assay (IELISA), a competitive ELISA (CELISA), and a fluorescence polarization assay (FPA) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free Canadian herds and sera from Argentina selected based on positive reactions in the buffered antigen plate agglutination test (BPAT) and the 2-mercaptoethanol (2-ME) test. In addition, sera from adult swine from which Brucella suis was isolated at least once for each farm of origin were evaluated. The IELISA, CELISA, and FPA specificity values were 99.9, 99.5, and 98.3%, respectively, and the IELISA, CELISA, and FPA sensitivity values relative to the BPAT and the 2-ME test were 98.9, 96.6, and 93.8%, respectively. Actual sensitivity was assessed by using 37 sera from individual pigs from which B. suis was cultured, and the values obtained were as follows: BPAT, 86.5%; 2-ME test, 81.1%; IELISA, 86.5%; CELISA, 78.5%; and FPA, 80.0%.Brucella suis occurs naturally in the smooth phase. The smooth Brucella spp. share certain smooth lipopolysaccharide (SLPS) epitopes, resulting in extensive serological cross-reactions. For this reason, conventional serological tests for swine brucellosis use antigens from Brucella abortus rather than B. suis, as shown by extensive testing for swine brucellosis in the United States (6). Conventional serological tests (16) and indirect enzyme-linked immunosorbent assays (IELISAs) (4, 16) cannot identify animals infected with Yersinia enterocolitica O:9 because of epitopes shared with Brucella spp. (16), resulting in lower specificity values than assays such as the competitive ELISA (CELISA) and the fluorescence polarization assay (FPA), which are capable of distinguishing animals infected with cross-reacting microorganisms (8, 9, 15) or vaccinated with B. abortus strain 19 (11) from animals infected with B. abortus or B. suis. The specificity of conventional serological agglutination tests was further reduced by nonspecific antibody thought to be immunoglobulin M (IgM) (16).Due to the uncertain nature of serological reactions to Brucella in pigs, the screening buffered antigen plate agglutination test (BPAT) (2) and the 2-mercaptoethanol (2-ME) agglutination test (7) are currently used for the detection of B. suis in infected herds. Only a few studies have described the development of primary binding assays for the detection of antibody to B. suis (17,20).More recently the IELISA, the CELISA, and the FPA developed for the diagnosis of bovine brucellosis (10, 14) have been validated for the presumptive diagnosis of swine brucellosis (15). The aims of the present study were to evaluate the IELISA, CELISA, and FPA for diagnosis of porcine brucellosis in Argentina, compared with the BPAT and 2-ME test used as diagnostic tests in Argentina, and to confirm previous data. MATERIALS AND METHODSNegative sera. Sera from Canadian pigs with no clinical or epidemiological evidence of brucellosis (B. suis has not been detected in Canad...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina

Paulo

Vigliocco

Ramondino

et al. 2000

Clin Diagn Lab Immunol

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“…Depending on the assay, they can be relatively easy to perform (STA) or more labor intensive (RIV). Sensitivities are variable (as low as 21% in RBT), however, specificities are usually quite high (96.8–99.3%) [94–96]. …”

Section: Diagnosticsmentioning

confidence: 99%

Comparisons of brucellosis between human and veterinary medicine

Hull

Schumaker

2018

Infection Ecology & Epidemiology

146

129

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Brucellosis is the world’s most widespread zoonosis, but also ranks as one of the seven most neglected diseases, according to the World Health Organization. Additionally, it is recognized as the world’s most common laboratory-acquired infection. There are a reported 500,000 incident cases of human brucellosis per year. However, true incidence is estimated to be 5,000,000 to 12,500,000 cases annually. Once diagnosed, focus is directed at treating individual patients with antibiotic regimes, yet overall neglecting the animal reservoir of disease. Countries with the highest incidence of human brucellosis are Syria (1,603.4 cases per 1,000,000 individuals), Mongolia (391.0), and Tajikistan (211.9). Surveillance on animal populations is lacking in many developed and developing countries. According to the World Animal Health Information Database, Mexico had the largest number of reported outbreaks, 5,514 in 2014. Mexico is followed by China (2,138), Greece (1,268), and Brazil (1,142). The majority of these outbreaks is Brucella abortus, the etiologic agent of bovine brucellosis. Brucellosis is an ancient disease that still plagues the world. There are still knowledge gaps and a need for better diagnostics and vaccines to make inroads towards control and eradication.

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“…Bu durum ELİsA testinin Bruceıla antikorlarını saptamada SAT'a göre daha duyarlı olduğunuda belirtmekte ve bu bulgular diğer araştırıcılara ait bulgularla da uygunluk göstermiştir (7,9,18,23,27,36,37).…”

Section: Tartışma Ve Sonuçunclassified

TÜRKİYE'DE SIGIR BRUCELLOsİs'İNİN İNsİDENSİ VE DENEYSEL OLARAK FARKLI AŞILARıN İMMUNOJENİTELERİNİN TAYİNİ ÜZERİNDE ARAŞTıR. MALAR'

Aydin¹,

Bisping²,

Izgür³

et al. 1987

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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A comparative study of ELISA and other methods for the detection of Brucella antibodies in bovine sera

Cited by 26 publications

References 9 publications

Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina

Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina

Comparisons of brucellosis between human and veterinary medicine

TÜRKİYE'DE SIGIR BRUCELLOsİs'İNİN İNsİDENSİ VE DENEYSEL OLARAK FARKLI AŞILARıN İMMUNOJENİTELERİNİN TAYİNİ ÜZERİNDE ARAŞTıR. MALAR'

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