A comparative study of Bulgarian and Turkish Asphodeline lutea root extracts: HPLC–UV profiles, enzyme inhibitory potentials and anti-proliferative activities against MCF-7 and MCF-10A cell lines

Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies

“…α-Amylase inhibitory activity was performed using Caraway–Somogyi iodine/potassium iodide (IKI) method 32 , 34 . Sample solution (25 µL, 2 mg/mL) was mixed with α-amylase solution (porcine pancreas, EC 3.2.1.1, Sigma, Saint Louis, MO) (50 µL) in phosphate buffer (pH 6.9 with 6 mM sodium chloride) in a 96-well microplate and incubated for 10 min at 37 °C.…”

A study on in vitro enzyme inhibitory properties of Asphodeline anatolica: New sources of natural inhibitors for public health problems

“…For example, the methanolic extracts of A. lutea roots from Bulgaria and Turkey were investigated in terms of enzyme inhibitory potentials, the activities were found to be 1.739-2.042 mgGALAEs/g extract for AChE, 1.745-2.938 mgGALAEs/g extract for BChE, 5.66-10.31 mgKAEs/g extract for tyrosinase, 0.268-0.526 mmolACAEs/g extract for ␣-amylase and 0.839-1.020 mmolACAEs/g extract for ␣-glucosidase (Lazarova et al, 2015). The observed inhibitory activities of A. lutea extracts may be explained with the presence of anthraquinones.…”

“…The extracts were potent enzyme inhibitor and antiproliferative agents Lazarova et al (2015) Eight Asphodeline root extracts Anthraquinones profiles, antioxidant and enzyme inhibitory properties…”

“…␣-Amylase inhibitory activity was performed using CarawaySomogyi iodine/potassium iodide (IKI) method (Lazarova et al, 2015) with some modifications. Sample solution (25 L) was mixed with ␣-amylase solution (50 L) in phosphate buffer (pH 6.9 with 6 mM sodium chloride) in a 96-well microplate and incubated for 10 min at 37 • C. After pre-incubation, the reaction was initiated with the addition of starch solution (50 L, 0.05%).…”

“…␣-Glucosidase inhibitory activity was performed by the previous method (Lazarova et al, 2015) with some modifications. Sample solution (50 L) was mixed with glutathione (50 L), ␣-glucosidase solution (50 L) in phosphate buffer (pH 6.8) and PNPG (50 L) in a 96-well microplate and incubated for 15 min at 37 • C. Similarly, a blank was prepared by adding sample solution to all reaction reagents without enzyme (␣-glucosidase) solution.…”