A study on in vitro enzyme inhibitory properties of Asphodeline anatolica: New sources of natural inhibitors for public health problems

Antihyperglycemic, antioxidant activities of two Acer palmatum cultivars, and identification of phenolics profile by UPLC-QTOF-MS/MS: New natural sources of functional constituents

“…Many medicinal plants have currently formed an integral part of primary health care in many developing countries across the world due to low side effects. What's more, about 20% of the drugs used worldwide come from plants (Zengin, 2016). Tannins, phenolic acids, flavonoids, terpenoids, alkaloids and steroids are generally considered as the major bioactive constituents in plants (Bueno et al, 2014;Sousa et al, 2016;Zhang et al, 2016).…”

Enzymatic assays and molecular modeling studies ofSchisandra chinensislignans and phenolics from fruit and leaf extracts

“…ChE inhibitory activity was measured using Ellman's method, as previously reported 28 . Sample solution (50 mL) was mixed with DTNB (5,5-dithio-bis(2-nitrobenzoic) acid) (125 mL) and AChE (acetylcholinesterase (Electric ell acetylcholinesterase, Type-VI-S, EC 3.1.1.7, Sigma-Aldrich)), or BChE (butyrylcholinesterase (horse serum butyrylcholinesterase, EC 3.1.1.8, Sigma-Aldrich)) solution (25 mL) in Tris-HCl buffer (pH 8.0) in a 96-well microplate and incubated for 15 min at 25 C. The reaction was then initiated with the addition of acetylthiocholine iodide (ATCI) or butyrylthiocholine chloride (BTCl) (25 mL).…”

“…-Amylase inhibition a-Amylase inhibitory activity was performed using CarawaySomogyi iodine/potassium iodide (IKI) method 28 . Sample solution (25 mL) was mixed with a-amylase solution (ex-porcine pancreas, EC 3.2.1.1, Sigma-Aldrich) (50 mL) in phosphate buffer (pH 6.9 with 6 mM sodium chloride) in a 96-well microplate and incubated for 10 min at 37 C. After pre-incubation, the reaction was initiated with the addition of starch solution (50 mL, 0.05%).…”

“…-Glucosidase inhibition a-Glucosidase inhibitory activity was performed by the previous method 28 . Sample solution (50 mL) was mixed with glutathione (50 mL), a-glucosidase solution (from Saccharomyces cerevisiae, EC 3.2.1.20, Sigma-Aldrich) (50 mL) in phosphate buffer (pH 6.8) and PNPG (4-N-trophenyl-a-D-glucopyranoside) (50 mL) in a 96-well microplate and incubated for 15 min at 37 C. Similarly, a blank was prepared by adding sample solution to all reaction reagents without enzyme (a-glucosidase) solution.…”

“…Tyrosinase inhibitory activity was measured using the modified dopachrome method with L-DOPA as substrate, as previously reported 28 with slight modifications. Sample solution (25 mL) was mixed with tyrosinase solution (40 mL) and phosphate buffer (100 mL, pH 6.8) in a 96-well microplate and incubated for 15 min at 25 C. The reaction was then initiated with the addition of L-DOPA (40 mL).…”