A Comparative Study of Blood Culture, Widal Test and Immunochromatographic Assay for Rapid Diagnosis of Typhoid Fever in a Tertiary Care Centre

Kumar, Maya; Velvizhi, G.; Sucilathangam, G.; Revathy, C.

doi:10.20546/ijcmas.2019.802.057

Cited by 3 publications

(3 citation statements)

References 4 publications

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“…Of them, 24(13.33%) cases were found positive for S. Typhi. Similar findings were also reported from India, 14% blood culture positive 13 and 12.22% culture positive 14 . The relative low rate of isolation from blood may be because of factors such as the bacteriostatic effect of antimicrobials which were already administered to some patients before blood culture was done, the time of sample collection, the type of culture medium used, the host immune response and the intracellular characteristics of S. Typhi 15 .…”

Section: Figures In Parenthesis Indicate Percentage (P Value=0000003743 P <005 By Chi-square Test) Discussionsupporting

confidence: 88%

A comparative study of blood culture and immunochromatographic assay for rapid diagnosis of typhoid fever

Akter¹,

Akter²,

Ahmed

et al. 2020

Bangladesh J Med Microbiol

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Background and objectives: Typhoid fever, caused by Salmonella Typhi, is an important cause of morbidity and mortality in many developing countries including Bangladesh. As early diagnosis of disease and prompt treatment are essential for optimal management, a rapid method is urgently needed. Blood culture is the gold standard for diagnosis of typhoid fever, but it is time consuming. Immunochromatographic test (ICT) is easily accessible, cheap and simple method for diagnosis of typhoid fever, especially in the resource poor countries like Bangladesh. Methodology: 180 samples were collected from suspected typhoid fever cases. This study was conducted to isolate the S. Typhi by blood culture and to detect IgM and IgG antibody to S. Typhi by ICT method. Results: Out of 180 samples, 105(58.33%) were positive in ICT and 24(13.33%) were positive in blood culture. Considering blood culture as gold standard, the sensitivity, specificity, positive predictive value of ICT was 91.67%, 46.79%, 20.95% and 97.33% respectively. Bangladesh J Med Microbiol 2020; 14 (1): 7-10

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Section: Figures In Parenthesis Indicate Percentage (P Value=0000003743 P <005 By Chi-square Test) Discussionsupporting

confidence: 88%

A comparative study of blood culture and immunochromatographic assay for rapid diagnosis of typhoid fever

Akter¹,

Akter²,

Ahmed

et al. 2020

Bangladesh J Med Microbiol

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show abstract

“…Peningkatan titer aglutinin H saja tanpa disertai peningkatan aglutinin O tidak dapat dipakai untuk mendiagnosis penyakit demam tifoid. Penyebab hal tersebut dapat terjadi ada 3, antara lain [8,9,12] [5]. Serum lebih baik digunakan untuk beberapa pemeriksaan karena serum tidak mengandung bahan-bahan dari luar seperti adanya penambahan antikoagulan sehingga komponen-komponen yang terkandung di dalam serum tidak terganggu aktifitas atau reaksinya [7,9].…”

Section: Pemakaian Antibiotikunclassified

“…Jika dilakukan penundaan maka disimpan pada suhu 2-8℃ selama 48 jam sebelum melakukan tes, pada suhu 4℃ selama 1 minggu serta untuk waktu yang cukup lama serum harus dibekukan. Darah yang belum membeku dengan baik kemudian dicentrifuge menyebabkan darah lisis sehingga menyulitkan pembacaan karena sampel akan menjadi keruh [9].…”

Section: Pemakaian Antibiotikunclassified

Gambaran Hasil Pemeriksaan Widal Menggunakan Serum Dan Plasma Edta Pada Suspek Demam Tifoid Di Rumah Sakit Umum Daha Husada Kota Kediri

Sabban¹,

Magdalena²,

Wardani³

et al. 2023

JUKEKE

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Typhoid fever is a systemic infection of the digestive system caused by the bacteria Salmonella typhi and Salmonella paratyphi. This disease is still a major health problem in the world. One of the tests for typhoid fever is the slide method widal test which measures antibodies to Salmonella typhi antigens. The most frequently used specimen is serum, but some hospitals use plasma as a substitute for serum. The purpose of this study was to compare the results of the widal examination using EDTA serum and plasma in suspected typhoid fever at Daha Husada General Hospital, Kediri City. The research design used a comparative study with a total population of 54 people. The study sample consisted of 15 patients using the quota sampling method. Analysis of research data used the Lilliefors test to test data normality and the Wilcoxon test to determine whether there was a comparison of the results of the widal examination using EDTA serum and plasma. The results showed that there were differences in the results of the widal examination using EDTA serum and plasma. The conclusion of the study was that there was a comparison of the results of the widal examination using EDTA serum and plasma in suspected typhoid fever at Daha Husada General Hospital, Kediri City.

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Immunogenicity Response of Mus musculus var. Balb/C after Immunization using Flagellin Salmonella typhi Serovar Semarang

Darmawati¹,

Gunawa²,

Wijayanti³

et al. 2022

Pakistan J. of Biological Sciences

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A Comparative Study of Blood Culture, Widal Test and Immunochromatographic Assay for Rapid Diagnosis of Typhoid Fever in a Tertiary Care Centre

Cited by 3 publications

References 4 publications

A comparative study of blood culture and immunochromatographic assay for rapid diagnosis of typhoid fever

A comparative study of blood culture and immunochromatographic assay for rapid diagnosis of typhoid fever

Gambaran Hasil Pemeriksaan Widal Menggunakan Serum Dan Plasma Edta Pada Suspek Demam Tifoid Di Rumah Sakit Umum Daha Husada Kota Kediri

Immunogenicity Response of Mus musculus var. Balb/C after Immunization using Flagellin Salmonella typhi Serovar Semarang

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