A comparative study of bioactive secondary metabolite production in diploid and tetraploid Echinacea purpurea (L.) Moench

Xu, Chuan-gui; Tang, Tie-xin; Chen, Rong; Liang, Chun-hui; Liu, Xiangyu; Wu, Chengyou; Yang, Youxin; Yang, Dan; Wu, Hong

doi:10.1007/s11240-013-0406-z

Cited by 69 publications

(54 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Data showed that accessions of A. ipaënsis and A. duranensis have higher concentrations of resveratrol than the two allotetraploids, which is a surprising result since many other studies showed differences between diploids and polyploids, the latter having better characteristics than the former (Fávero et al, 2009;Xu et al, 2014;Michelotto et al, 2015;Leal-Bertioli et al, 2015, 2017. The lack of polyploidy superiority has been previously observed for Arabidopsis thaliana and Pyrus communis, in which only a few metabolites differ between diploids and synthetic autotetraploids.…”

Section: Resveratrol Contentmentioning

confidence: 59%

Research Article Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

et al. 2017

View full text Add to dashboard Cite

ABSTRACT.Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase genes in four Arachis genotypes. Two diploids and two tetraploid were analyzed. Contents of resveratrol on non-and UV-treated leaves were estimated using HPLC. Resveratrol synthase (RS) was analyzed using RT-qPCR with primers developed in this study. Sequences of six Arachis species were amplified using two degenerated primer pairs that were designed based on Arachis and general RS available at GenBank. Those sequences were used to qPCR primers design. Test and control leaves were collected from plants cultivated in greenhouse and three biological replicates were evaluated for each genotype. The synthesis of resveratrol in leaves was induced by treatment with UV for 2.5 h. All genotypes studied synthesized resveratrol. Concentrations ranged from 193.66 µg/g in synthetic allotetraploid to 371.97 µg/g in A. duranensis. Natural and induced allotetraoploids showed lower levels of resveratrol than their diploid parents. Untreated samples did not produce significant amounts of resveratrol. The analysis of resveratrol content and levels of RS mRNA allowed the identification of one gene induced by the UV treatment. The data showed different amounts of RS in the different genotypes suggesting early and late response to the UV induction in the different species. The understanding of the variation found among species will help to identify species that have high resveratrol content and their ideal pos-induction times. This also will allow analysis of other tissues where high levels resveratrol would be very important, such as in seeds.

show abstract

Section: Resveratrol Contentmentioning

confidence: 59%

Research Article Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Fourteen aneuploids had higher whole-plant chlorogenic acid contents than parents, with A21 exhibiting the highest content which was approximately five times higher than the contents of diploid and triploid parents. Enhanced production of cichoric acid and chlorogenic acid were also reported in E. purpurea tetraploid plants compared with diploid individuals [24,25,45], but not to the extent found in aneuploids, e.g., Abdoli detected that leaves of tetraploid plants had 45% and 71% more cichoric acid and chlorogenic acid than diploid plants [45].…”

Section: Discussionmentioning

confidence: 79%

“…We have recently made some progress on polyploidy induction, including tetraploid [21], triploid [22], and octoploid [23] organisms. Polyploidy presented higher bioactive compounds contents than diploidy [24,25]. Now, as the karyotypic particularity of aneuploidy, developing new aneuploid lines with high contents of functional compounds may be innovative and promising.…”

Section: Introductionmentioning

confidence: 99%

In vitro cloning potential and phytochemical evaluations of aneuploid individuals produced from reciprocal crosses between diploid and triploid in Echinacea purpurea L.

Jiang

Ren

et al. 2017

Acta Soc Bot Pol

Self Cite

View full text Add to dashboard Cite

Aneuploidy often presents large variations in morphology, physiology, biochemistry, and genetics owing to karyotypic imbalance. This study aimed to evaluate the efficacy of aneuploid breeding in Echinacea purpurea L, an important medicinal plant. Reciprocal crosses between diploid and triploid plants were performed to generate aneuploid plants. Cross with triploid as female parent resulted in increased production of aneuploid individuals (19 of 23; 82.61%), while using diploid as female parent yielded much higher percentage of diploid progenies (130 of 133; 97.74%). Each aneuploid had particular karyotypic characteristics compared to the parents. The proportions of median, submedian, and subterminal centromere location chromosomes in gross chromosomes among aneuploids and two parents showed large variations. Although aneuploids had relatively lower adventitious bud regeneration rates than their parents, almost half of them looked morphologically normal, with high survival rates when transplanted to ex vitro conditions. Among the bioactive compounds assessed, cichoric acid and chlorogenic acid contents were extremely encouraging. Most aneuploids had higher cichoric acid and chlorogenic acid contents than their parents. For example, A2 had the highest cichoric acid content of 21.98 mg/g dry weight, more than twice the values of diploid and triploid. Meanwhile, A21 had the highest chlorogenic acid content of 1.84 mg/g, approximately five times more than the parental values. Eleven superior aneuploid lines were successfully screened as breeding candidates. The present findings indicated E. purpurea is highly tolerant of karyotypic imbalance and aneuploid plants could serve as prospective breeding resources in E. purpurea.

show abstract

“…In nitrogen deficient plants, the activity of phenyl alanine lyase (PAL) which is a key compound in flavonoids synthesis pathway, is positively correlated with carbonbase compounds (Zhang & Liu, 2015;Xu et al, 2014), is always higher than that in nitrogen enough plants and has more polyphenolic compounds to synthesis flavonoids (Kováčik & Bačkor, 2007). Therefore, plants with nitrogen deficiency will increase flavonoids concentration (Liu et al,2010a;Liu et al, 2010b;Ibrahim et al, 2011).…”

Section: Organic and Inorganic N On Flavonoids Concentration Of Pnotmentioning

confidence: 99%

Effect of Organic-Inorganic N Sources on Growth, NPK Nutrients and Secondary Metabolites of Panax Notoginseng (Burk.) F. H. Chen

Yang

Guo

et al. 2017

Emir J Food Agric

View full text Add to dashboard Cite

The effects of organic and inorganic N combined application on P.notoginseng cultivation are poorly known. For this reason, we have carried out a pot culture experiment in 2010. The treatments respectively were (1) control (no N input, CK), (2) organic N (ON), (3) half organic N and half inorganic N (1/2ON+ 1/2IN), (4) inorganic N (IN). Plant growth, NPK and secondary metabolites concentration were assayed. Results showed that compared to CK, ON increased plant height, N, P concentration and accumulation and total saponins concentration, but reduce total flavonoids concentration. IN increased shoot and root biomass, N concentration and accumulation, K accumulation, but reduce total flavonoids and saponins concentration. 1/2ON+ 1/2IN had the highest hair root length, root diameter, biomass, N concentration and accumulation, K concentration in roots, total saponins concentration, but had the lowest K concentration in shoots, total flavonoids concentration in shoots and roots. Correlation analysis showed total flavonoids concentration was negative correlated with N concentration and accumulation, K concentration in shoots, but was positive correlated K concentration in roots. Total saponins concentration was negative correlated with K concentration in shoots whereas positive in roots, and was negative correlated with total flavonoids concentration. Totally, organic and inorganic N combined application with the ratio of 50/50 is the best way for growth and saponins of P.notoginseng.

show abstract

A comparative study of bioactive secondary metabolite production in diploid and tetraploid Echinacea purpurea (L.) Moench

Cited by 69 publications

References 32 publications

Research Article Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

Research Article Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

In vitro cloning potential and phytochemical evaluations of aneuploid individuals produced from reciprocal crosses between diploid and triploid in Echinacea purpurea L.

Effect of Organic-Inorganic N Sources on Growth, NPK Nutrients and Secondary Metabolites of Panax Notoginseng (Burk.) F. H. Chen

Contact Info

Product

Resources

About