A comparative proteomic analysis provides insights into pigment biosynthesis in brown color fiber

Li, Yanjun; Zhang, Xinyu; Wang, Fuxin; Yang, Chun-Lin; Liu, Feng; Xia, Gui-Xian; Sun, Jie

doi:10.1016/j.jprot.2012.10.005

Cited by 40 publications

(38 citation statements)

References 53 publications

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“…Recent studies have detected the expression of structural genes involved in the flavonoid (including anthocyanin) synthetic pathway, such as CHS , CHI , F3H , DFR , ANS , and ANR , in the cotton fibers of several cotton cultivars [49], [50]. Despite no visible anthocyanin accumulation, the expression of structural genes was detected in the leaves of CCRI 24 plants grown in light conditions in our study (data not shown).…”

Section: Resultscontrasting

confidence: 43%

The Promoter Structure Differentiation of a MYB Transcription Factor RLC1 Causes Red Leaf Coloration in Empire Red Leaf Cotton under Light

et al. 2013

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The red leaf coloration of Empire Red Leaf Cotton (ERLC) (Gossypium hirsutum L.), resulted from anthocyanin accumulation in light, is a well known dominant agricultural trait. However, the underpin molecular mechanism remains elusive. To explore this, we compared the molecular biological basis of anthocyanin accumulation in both ERLC and the green leaf cotton variety CCRI 24 (Gossypium hirsutum L.). Introduction of R2R3-MYB transcription factor Rosea1, the master regulator anthocyanin biosynthesis in Antirrhinum majus, into CCRI 24 induced anthocyanin accumulation, indicating structural genes for anthocyanin biosynthesis are not defected and the leaf coloration might be caused by variation of regulatory genes expression. Expression analysis found that a transcription factor RLC1 (Red Leaf Cotton 1) which encodes the ortholog of PAP1/Rosea1 was highly expressed in leaves of ERLC but barely expressed in CCRI 24 in light. Ectopic expression of RLC1 from ERLC and CCRI 24 in hairy roots of Antirrhinum majus and CCRI 24 significantly enhanced anthocyanin accumulation. Comparison of RLC1 promoter sequences between ERLC and CCRI 24 revealed two 228-bp tandem repeats presented in ERLC with only one repeat in CCRI 24. Transient assays in cotton leave tissue evidenced that the tandem repeats in ERLC is responsible for light-induced RLC1 expression and therefore anthocyanin accumulation. Taken together, our results in this article strongly support an important step toward understanding the role of R2R3-MYB transcription factors in the regulatory menchanisms of anthocyanin accumulation in red leaf cotton under light.

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Section: Resultscontrasting

confidence: 43%

The Promoter Structure Differentiation of a MYB Transcription Factor RLC1 Causes Red Leaf Coloration in Empire Red Leaf Cotton under Light

et al. 2013

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“…Cotton protein extraction for proteomics studies has largely been performed using modifications on the traditional phenol extraction method as reported [2,5,7,8,10,11,13,20,22,24,25] and TCA extraction as described by Pang et al [6]. As cotton fiber tissue matures, however, the protein content decreases, the cell wall thickens and interfering content, such as polysaccharides, polyphenolic compounds, pectin, lipids and waxes, is present, which substantially affect downstream proteomic applications [7,46].…”

Section: Resultsmentioning

confidence: 99%

“…During primary wall elongation (within 20 days post anthesis (dpa)) and secondary wall deposition and thickening (from 20 to 35 dpa), sizeable amounts of polysaccharide components are synthesized and deposited, producing a cell wall 3 to 4 µm thick, made up of more than 94% cellulose [3,7,8]. These advantageous features make cotton fiber an excellent single-celled model for studying the molecular mechanisms of plant cell elongation, cell wall development and cellulose biosynthesis [2,5,9,10,11]. …”

Section: Introductionmentioning

confidence: 99%

“…Several comparative proteome and transcriptome studies during different stages of cotton fiber development have been reported [2,5,8,11,12]. In addition, the transcriptome and proteome comparisons between reduced fiber and fiberless mutants and their respective parental wild-types (WT) have also been conducted [6,14,15,16,17,18,19,20,21,22].…”

Section: Introductionmentioning

confidence: 99%

“…However, this method was unsuitable for two-dimensional gel electrophoresis due to the horizontal and vertical streaking and smearing caused by the phenolic and other contaminants co-extracted with proteins [7,23]. In recent years, cotton fiber proteins have been mainly extracted with modifications on the phenol-based procedure [2,5,7,8,10,11,13,20,22,24,25] and by the trichloroacetic acid extraction method, as described by Pang et al [6]. However, there have not been any reports of the successful extraction of cotton fiber proteins for shotgun proteomics from maturing fiber tissues, for example fiber stages after 30 dpa.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers

et al. 2016

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The distinct stages of cotton fiber development and maturation serve as a single-celled model for studying the molecular mechanisms of plant cell elongation, cell wall development and cellulose biosynthesis. However, this model system of plant cell development is compromised for proteomic studies due to a lack of an efficient protein extraction method during the later stages of fiber development, because of a recalcitrant cell wall and the presence of abundant phenolic compounds. Here, we compared the quality and quantities of proteins extracted from 25 dpa (days post anthesis) fiber with multiple protein extraction methods and present a comprehensive quantitative proteomic study of fiber development from 10 dpa to 25 dpa. Comparative analysis using a label-free quantification method revealed 287 differentially-expressed proteins in the 10 dpa to 25 dpa fiber developmental period. Proteins involved in cell wall metabolism and regulation, cytoskeleton development and carbohydrate metabolism among other functional categories in four fiber developmental stages were identified. Our studies provide protocols for protein extraction from maturing fiber tissues for mass spectrometry analysis and expand knowledge of the proteomic profile of cotton fiber development.

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Molecular analysis of structural genes involved in flavonoids biosynthesis in naturally colored cotton

Canavar

Rausher

2020

Crop Science

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In order to facilitate the development of cotton (Gossypium hirsutum L.) strains with colored fiber, the genetic and developmental control of flavonoids in fibers must be understood. The major goal of this study was to determine whether anthocyanidins accumulate in three different natural colored cotton fibers during their development, and to document the expression levels of flavonoid genes necessary for the production of anthocyanidins to determine if differences in expression levels reflect differences in anthocyanidin production. Most flavonoid structural genes were more highly expressed during fiber elongation than before the beginning of secondary cell wall formation. High performance liquid chromatography (HPLC) analyses showed that the abundance of anthocyanidins was higher in brown fibers than in green or white fibers. Anthocyanidins were present at high levels in fibers, starting at 20 d post‐anthesis of fiber development, which is almost at the end of the fiber elongation stage. Brown cotton contains higher delphinidin levels than of green and white cotton. This pattern is consistent with greater expression of anthocyanin pathway genes in brown fibers. In naturally colored cotton, the enzyme Lar (leucoanthocyanidin reductase) converted leucoanthocyanidins to catechin, which are proanthocyanins (PAs) precursor subunits. It is suggested that failure to produce anthocyanins is likely due to a combination of insufficient expression of Ufgt (UDP‐glucose: flavonoid 3‐O‐glucosyltransferase) and high expression of Lar and Anr (anthocyanidin reductase).

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A comparative proteomic analysis provides insights into pigment biosynthesis in brown color fiber

Cited by 40 publications

References 53 publications

The Promoter Structure Differentiation of a MYB Transcription Factor RLC1 Causes Red Leaf Coloration in Empire Red Leaf Cotton under Light

The Promoter Structure Differentiation of a MYB Transcription Factor RLC1 Causes Red Leaf Coloration in Empire Red Leaf Cotton under Light

Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers

Molecular analysis of structural genes involved in flavonoids biosynthesis in naturally colored cotton

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