A comparative proteomic analysis of rice seedlings under various high-temperature stresses

Han, Feng; Chen, Hui; Li, Xiaojuan; Yang, Mo; Liu, Gongshe; Shen, Shihua

doi:10.1016/j.bbapap.2009.07.013

Cited by 106 publications

(91 citation statements)

References 46 publications

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“…High temperature is one of the most important abiotic stresses that reduce crop yield and quality [3,4]. Rice production is likely to be affected severely by an increase in mean global temperature [5,6]. The overall global temperature has steadily increased in recent decades due to rapid increases in atmospheric greenhouse gas concentrations [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Lee et al [8] found that HSPs and energy- and metabolism-associated proteins were the major proteins affected by a high temperature of 42°C in leaves. In another study, lignification-related proteins were regulated by high temperature, and distinct proteins related to protection were up-regulated at different high temperatures, indicating that different strategies were adopted at different levels of high temperature: the higher the temperature, the greater the involvement of the protection machineries [6]. …”

Section: Introductionmentioning

confidence: 99%

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Phosphoproteins regulated by heat stress in rice leaves

Chen

Zhang

et al. 2011

Proteome Sci

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BackgroundHigh temperature is a critical abiotic stress that reduces crop yield and quality. Rice (Oryza sativa L.) plants remodel their proteomes in response to high temperature stress. Moreover, phosphorylation is the most common form of protein post-translational modification (PTM). However, the differential expression of phosphoproteins induced by heat in rice remains unexplored.MethodsPhosphoprotein in the leaves of rice under heat stress were displayed using two-dimensional electrophoresis (2-DE) and Pro-Q Diamond dye. Differentially expressed phosphoproteins were identified by MALDI-TOF-TOF-MS/MS and confirmed by Western blotting.ResultsTen heat-phosphoproteins were identified from twelve protein spots, including ribulose bisphos-phate carboxylase large chain, 2-Cys peroxiredoxin BAS1, putative mRNA binding protein, Os01g0791600 protein, OSJNBa0076N16.12 protein, putative H(+)-transporting ATP synthase, ATP synthase subunit beta and three putative uncharacterized proteins. The identification of ATP synthase subunit beta was further validated by Western-blotting. Four phosphorylation site predictors were also used to predict the phosphorylation sites and the specific kinases for these 10 phosphoproteins.ConclusionHeat stress induced the dephosphorylation of RuBisCo and the phosphorylation of ATP-β, which decreased the activities of RuBisCo and ATP synthase. The observed dephosphorylation of the mRNA binding protein and 2-Cys peroxiredoxin may be involved in the transduction of heat-stress signaling, but the functional importance of other phosphoproteins, such as H+-ATPase, remains unknown.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phosphoproteins regulated by heat stress in rice leaves

Chen

Zhang

et al. 2011

Proteome Sci

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“…The responses of rice seedlings to different hightemperature stresses at 35, 40 and 45°C for 48 h. At 35°C, some protective mechanisms were activated to maintain the photosynthetic capability. At 40°C, antioxidative pathways were also active so, the overall protein content increased with temperature (Han et al, 2009). When seven day old rice seedlings encountered high-temperature stress at 45°C, in addition to those induced at 35°C and 40°C, heat shock proteins were effectively induced so the protein content also increased as temperature increased.…”

Section: Protein Contentmentioning

confidence: 81%

Biochemical and physiological constituents and their correlation in wheat (Triticum aestivum L.) genotypes under high temperature at different development stages

Ramani¹,

Mandavia²,

Dave³

et al. 2017

Int. J. Plant Physiol. Biochem.

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Wheat is the most popular and staple food for millions of people. It is severely affected by heat stress in many countries. Vegetative growth and reproductive phases in wheat differ in their sensitivity to temperature. Heat tolerant (GW-190) and Heat susceptible (J-2010-11) genotypes grown up to tillering and grain filling stages and Heat treatments (40°C and 45°C for 2h and 4h) were given using Heating House. After heat treatment samples were collected, the biochemical and physiological analysis such as Protein, Proline, Glycine betaine, Membrane stability, Relative water content, Germination percent, Seed vigour and Heat tolerant index were performed. Protein, proline and glycine betaine were found significantly highest 34.06 mg/g Fr. Wt., 13.70 mg% and 902.24 µg/F. wt respectively in heat tolerant genotype GW-190 at 45°C for 4 h at tillering stage. Membrane stability and relative water content were found significantly highest 56.83% and 86.15 % respectively in heat tolerant genotype GW-190 at tillering stage. Germination percent, Seed vigor and Heat tolerance index were found higher in control group of Heat tolerant GW-190 genotypes. Where, all the biochemical and physiological contents were found lower in heat susceptible J-2010-11 genotype. From the above results it was concluded that GW-190 was heat tolerant genotypes which is suitable for grown in area of high temperature and J-2010-11 was found heat susceptible.

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“…Although anatomical and physiological properties of the root system are not easily accessible in field experiments, root growth, activity and response to abiotic stresses must be borne in mind for identifying suitable genotypes [27,141,174]. Besides basic characteristics of unstressed plants, especially stress-inducible adaptations in gene expression, protein pattern and physiological properties are important for the stress susceptibility of a genotype [113,162,163]. Heat waves and drought periods occur often simultaneously and should therefore also be addressed in combination in selection procedures [169][170][171].…”

Section: Discussionmentioning

confidence: 99%

“…Different types of tissues are studied: roots [141,154,159], root nodules [161], leaves [138,160], roots and leaves compared at seedling stage [157], roots, flag leaves and spikelets at reproductive stage [151]. As the high temperature is particularly detrimental during the reproductive stage, besides the studies on leaf proteome under heat stress in rice [113,162], wheat [163] radish [142], alfalfa [164] and stromal proteins in agave [165], special attention is paid to the reproductive phase [166], source-sink interactions at grain filling [167], grain development and composition [155,160] in cereals, and protein composition of soybean seeds [168] formed under unfavorable temperature conditions. Relatively few proteomic studies deal with combined drought and heat stress [169][170][171].…”

Section: Proteomics In Search Of Molecular Markers For Assisted Selecmentioning

confidence: 99%

Selection and Breeding of Suitable Crop Genotypes for Drought and Heat Periods in a Changing Climate: Which Morphological and Physiological Properties Should Be Considered?

2016

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Selection and breeding of genotypes with improved drought/heat tolerance become key issues in the course of global change with predicted increased frequency of droughts or heat waves. Several morphological and physiological plant traits must be considered. Rooting depth, root branching, nutrient acquisition, mycorrhization, nodulation in legumes and the release of nutrients, assimilates or phytohormones to the shoot are relevant in root systems. Xylem embolism and its repair after a drought, development of axillary buds and solute channeling via xylem (acropetal) and phloem (basipetal and acropetal) are key processes in the stem. The photosynthetically active biomass depends on leaf expansion and senescence. Cuticle thickness and properties, epicuticular waxes, stomatal regulation including responses to phytohormones, stomatal plugs and mesophyll resistance are involved in optimizing leaf water relations. Aquaporins, dehydrins, enzymes involved in the metabolism of compatible solutes (e.g., proline) and Rubisco activase are examples for proteins involved in heat or drought susceptibility. Assimilate redistribution from leaves to maturing fruits via the phloem influences yield quantity and quality. Proteomic analyses allow a deeper insight into the network of stress responses and may serve as a basis to identify suitable genotypes, although improved stress tolerance will have its price (often lowered productivity under optimal conditions).

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A comparative proteomic analysis of rice seedlings under various high-temperature stresses

Cited by 106 publications

References 46 publications

Phosphoproteins regulated by heat stress in rice leaves

Phosphoproteins regulated by heat stress in rice leaves

Biochemical and physiological constituents and their correlation in wheat (Triticum aestivum L.) genotypes under high temperature at different development stages

Selection and Breeding of Suitable Crop Genotypes for Drought and Heat Periods in a Changing Climate: Which Morphological and Physiological Properties Should Be Considered?

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