Wheat is the most popular and staple food for millions of people. It is severely affected by heat stress in many countries. Vegetative growth and reproductive phases in wheat differ in their sensitivity to temperature. Heat tolerant (GW-190) and Heat susceptible (J-2010-11) genotypes grown up to tillering and grain filling stages and Heat treatments (40°C and 45°C for 2h and 4h) were given using Heating House. After heat treatment samples were collected, the biochemical and physiological analysis such as Protein, Proline, Glycine betaine, Membrane stability, Relative water content, Germination percent, Seed vigour and Heat tolerant index were performed. Protein, proline and glycine betaine were found significantly highest 34.06 mg/g Fr. Wt., 13.70 mg% and 902.24 µg/F. wt respectively in heat tolerant genotype GW-190 at 45°C for 4 h at tillering stage. Membrane stability and relative water content were found significantly highest 56.83% and 86.15 % respectively in heat tolerant genotype GW-190 at tillering stage. Germination percent, Seed vigor and Heat tolerance index were found higher in control group of Heat tolerant GW-190 genotypes. Where, all the biochemical and physiological contents were found lower in heat susceptible J-2010-11 genotype. From the above results it was concluded that GW-190 was heat tolerant genotypes which is suitable for grown in area of high temperature and J-2010-11 was found heat susceptible.
Herein, we have studied the self-assembling and aggregation properties of Homogentisic acid (HA), N-acetyl aspartic Acid (NAA) and Isovaleric acid (IVA) with the aim to understand the effect of aggregation of these metabolites on the toxicities associated with rare metabolic diseases caused by their accumulation. To our surprise, we noted aggregation of HA, NAA and IVA follow amyloidogenic pathway and as these metabolites are aged small globules tend to merge and form fibrillar aggregates. These assemblies were characterized by conventional microscopy tools and their amyloidogenic nature was assessed by Thioflavin T binding assays. The cytotoxicity analysis by MTT assay suggest cellular viability was decreased in presence of metabolites in a dose dependent manner implicating their cytotoxic nature. Hence, the results presented in the manuscript are of significant interest as they can be of great practical implications in understanding the etiology/pathophysiology of rare metabolic diseases caused by the accumulation of these metabolites namely Alkaptonuria, Canavan diseases and Isovaleric academia from an amyloid perspective and may have possible implications in its therapeutic cure in future.
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