A Comparative Metabolomics Approach Reveals Early Biomarkers for Metabolic Response to Acute Myocardial Infarction

Ali, Sara E.; Farag, Mohamed A.; Holvoet, Paul; Hanafi, Rasha S.; Gad, Mohamed Z.

doi:10.1038/srep36359

Cited by 72 publications

(56 citation statements)

References 62 publications

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“…For instance, Ali et al. have described how HS‐SPME sampling with the use of DVB/CAR/PDMS coated fibers can be highly useful for extraction and further analysis by GC–MS of volatile metabolites, while avoiding derivatization step . Their data confirmed that an increase in acetone levels in the serum of some ST‐elevation myocardial infarction STEMI patients can be observed when compared to healthy controls.…”

Section: Spme In Biomedical and Clinical Studiesmentioning

confidence: 94%

“…The ability to simultaneously determine changes in the hundreds of metabolites in a serum sample enables researchers to measure the multidirectional metabolic responses of living systems to pathophysiological stimuli. For instance, Ali et al have described how HS-SPME sampling with the use of DVB/CAR/PDMS coated fibers can be highly useful for extraction and further analysis by GC-MS of volatile metabolites, while avoiding derivatization step [55]. Their data confirmed that an increase in acetone levels in the serum of some ST-elevation myocardial infarction STEMI patients can be observed when compared to healthy controls.…”

Section: Head Space Spme In Untargeted Metabolomicsmentioning

confidence: 95%

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Application of solid‐phase microextraction in current biomedical research

Roszkowska

Miękus

Bączek

2018

J of Separation Science

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Extraction of endogenous compounds and drugs and their corresponding metabolites from complex matrices, such as biofluids and solid tissues, requires adequate analytical approach facilitating qualitative and quantitative analysis. To this end, solid‐phase microextraction has been introduced as modern technology that is capable of efficient and high‐throughput extraction of compounds due to its ability to amalgamate sampling, extraction, and pre‐concentration steps, while requiring minimal use of organic solvents. The ability of solid‐phase microextraction to enable analyses on small‐volume biological samples and growing availability of biocompatible solid‐phase microextraction coatings make it a highly useful technology for variety of applications. For example, solid‐phase microextraction is particularly useful for identifying biomarkers in metabolomics studies, and it can be successfully applied in pharmaceutical and toxicological studies requiring the fast and sensitive determination of drug levels, especially those that are present at low levels in biological matrices such as plasma, urine, saliva, and hair. Moreover, solid‐phase microextraction can be directly applied in in vivo studies because this extraction technique is non‐exhaustive and its biocompatible probes offer minimal invasiveness to the analyzed system. In this article, we review recent progress in well‐established solid‐phase microextraction technique for in vitro and in vivo analyses of various metabolites and drugs in clinical, pharmaceutical, and toxicological applications.

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Section: Spme In Biomedical and Clinical Studiesmentioning

confidence: 94%

Section: Head Space Spme In Untargeted Metabolomicsmentioning

confidence: 95%

Application of solid‐phase microextraction in current biomedical research

Roszkowska

Miękus

Bączek

2018

J of Separation Science

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“…For assessment of biological replicates, 100 μl of the methanolic extract was aliquoted in screw cap vials and left to evaporate under nitrogen gas stream until complete dryness. Derivatization was carried out as mentioned in Ali, Farag, Holvoet, Hanafi, and Gad ().…”

Section: Methodsmentioning

confidence: 99%

Effect of grilling as processing method on Zea mays (corn) metabolites composition as analyzed via SPME GC‐MS and chemometrics

Farag

Ammar

Gendy

et al. 2019

J Food Process Preserv

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Zea mays L. is a worldwide economical crop that is used as a food for man or as animal fodder owing to its rich nutritive composition. Despite being extensively studied, grilling effect on corn flavor and chemistry has yet to be reported. Impact of grilling at different degrees, light grilled corn (LGC) versus heavy grilled corn (HGC) on metabolite profile of Z. mays was assessed in this study using a holistic approach via SPME GC‐MS and multivariate data analysis. A total of 39 volatiles were identified post‐grilling to encompass pyrazines, phenols, pyrroles, furans, and aldehydes, with pyrazines likely to mediate for its flavor present as major constituents (80.9% in HGC vs. 68.4% in LGC). GC‐MS was further utilized to localize nutrients (viz., sugars, sugar alcohols, fatty acids, and organic acids) revealing that HGC is enriched in sugar 89.5% concurrent with lower organic acid levels imparting a sweetless acidulated taste. Practical applications This study presents the first approach utilizing SPME and GC‐MS to characterize the grilling impact as a processing method on corn metabolome as analyzed using chemometric tools. The study rationalize for corn improved taste and flavor upon grilling based on detailed chemical analyses.

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“…Recent report unveiled that NaHS-induced anti-atherosclerotic effects could also be partially through up-regulating ATP-Binding Cassette Transporter A1 (ABCA1) [116]. In addition to the therapeutic effects of H 2 S, H 2 S could be a biomarker for ST-elevation myocardial infarction and unstable angina [117]. …”

Section: Evidence Related To Atherosclerosis Prevention and Treatmentmentioning

confidence: 99%

Atherosclerosis and the Hydrogen Sulfide Signaling Pathway – Therapeutic Approaches to Disease Prevention

Zj¹,

Wu²,

Guo³

et al. 2017

Cell Physiol Biochem

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Hydrogen sulfide (H₂S) is now admitted as a third gasotransmitter together with nitric oxide (NO) and carbon monoxide (CO), albeit it was originally considered as a foul and poisonous gas. Endogenous H₂S production in mammalian cells is counting on the three enzymes acting on cysteine. Involvement of H₂S in various physiological and pathological processes has been extensively studied in the last fifteen years. Mounting evidence suggests that H₂S is able to protect against atherosclerosis development and progression. Exogenous H₂S supplement has salutary effects on atherogenesis, and reduction of the endogenous H₂S level accelerates atherosclerosis. The anti-atherosclerotic mechanisms of H₂S have been descried in different aspects, including endothelium preservation, antioxidative action, anti-inflammatory responses, vasorelaxation, regulation of ion channels, etc. However, further investigation is still needed to help us gain more insights into the fundamental underlying mechanisms, and that will allow us to design better therapeutic applications of H₂S in the treatment of atherosclerosis.

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A Comparative Metabolomics Approach Reveals Early Biomarkers for Metabolic Response to Acute Myocardial Infarction

Cited by 72 publications

References 62 publications

Application of solid‐phase microextraction in current biomedical research

Application of solid‐phase microextraction in current biomedical research

Effect of grilling as processing method on Zea mays (corn) metabolites composition as analyzed via SPME GC‐MS and chemometrics

Atherosclerosis and the Hydrogen Sulfide Signaling Pathway – Therapeutic Approaches to Disease Prevention

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