A comparative in vitro study of antibody binding to different stages of the hookwormAncylostoma caninum

Abstract

The Indirect Fluorescent Antibody Technique (IFAT) and the Indirect Immuno Peroxidase Technique (IIPT) have been applied to cryostat sections and intact stages of the hookworm species Ancylostoma caninum with sera from infected dogs. Especially the role of the body surface (= cuticle (cortex, matrix, basal layer) and hypodermis) in immunity was studied. Using cryostat sections and dead intact stages as the antigen, specific antibody binding was demonstrated round the ovum membrane and the cuticle of all stages of this hookworm species. Cryostat sections of adult worms showed, that it probably is not the cuticle itself that is antigenic, but that the specific reaction that is observed consisted of a layer, covering the cortex of the cuticle. Infective and parasitic living stages, however, showed no antibody binding in contrast to the free-living stages in which specific antibody binding was demonstrated.

Excretory–secretory products of helminth parasites: effects on host immune responses

“…Indirect evidence for the release of surface antigens of Ancylostoma caninum (Klaver-Wesseling, Vetter & Visser, 1978; and T. canis (Smith, Quinn, Kusel & Girdwood, 1981) is provided by the observation that antibody bound to the surface of these nematodes is shed rapidly in vitro. This release of surface molecules was found to be temperature dependent and inhibited by anti-metabolites.…”

Immunohistochemical localization of excretory/secretory antigens in adult Ancylostoma caninum using monoclonal antibodies and infected human sera

“…Binding was strong in the amphidial glands, moderate in the oesophageal and excretory glands and much weaker with the epicuticle and other structures (Figure 4a). In infected dogs, the cuticle of all developmental stages of A. caninum is a prime target of immune responses (Klaver-Wesseling, Vetter & Visser 1978, but our studies suggest that, at least in infected humans who develop EE, antigens from amphidial glands of adult worm stimulate more intense responses. This may reflect the hypersensitivity to worm secretions that probably underlies the pathogenesis of EE, or simply that the worm is not in its usual host.…”