A Comparative Electron Microscopic Study on the Morphogenesis of Canine Distemper and Rinderpest Viruses

“…The presence of inclusion bodies both in the nucleus and cytoplasm is consistent with such findings in MDI~ virus infections (1,27,30,32,35,39). The presence of inclusion bodies both in the nucleus and cytoplasm is consistent with such findings in MDI~ virus infections (1,27,30,32,35,39).…”

Section: Discussionsupporting

confidence: 83%

Sporadic bovine meningo-encephalitis-isolation of a paramyxovirus

Bachmann

Meulen

Jentsch

et al. 1975

Archives of Virology

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Isolation of a viral agent (107) directly from brain explants of a 15-month-old heifer with symptoms of a sporadic encephalomyelitis is described. The virus shares properties with the paramyxovirus family. It grows in a variety of cell cultures from different species, and induces nuclear and cytoplasmic inclusion bodies in infected cells. Nucleocapsids measuring 17 nm in diameter were found in the nucleus and cytoplasm of these cells when studied electron microscopically, thus indicating a close relationship of the agent to the measles-distemper-rinderpest group. No infectious virus was released from infected cells, although alignment of nucleocapsids was observed beneath the cell membrane, and no hemagglutinating activity could be detected with the methods employed. The 107 agent was compared serologically with parainfluenza viruses type 1, 2 and 3, simian virus 5, mumps and Newcastle disease virus (NDV), two bovine respiratory syncytial viruses and measles/subacute sclerosing panencephalitis, distemper and rinderpest viruses, always using 107 virus infected CV1 cells and antiserum of the different viruses in indirect FA tests. Positive FA reactions were observed only with two sera obtained from SSPE patients with high antibody titer to SSPE virus, and with one rabbit-anti-rinderpest serum. The titers of these sera to 107 virus, however, were significantly lower than those against homologous viruses. Five out of 9 sera from randomly selected healthy cattle showed antibody titers between 1:10 and 1:80 to 107 virus in FA tests. The significance of these results is discussed with respect to the epidemiology of SSPE in children and its possible implication with rinderpest in Europe.

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“…The particles differed in form, but usually were oval or circular, with a size ranging from 200 to 500 nm. Other inclusions were formed by interwoven filaments or tubules, corresponding in size to the nucleocapsids of this virus which have been described by others [2,10,16,19]. Minimal cell damages were found in association with intracytoplasmic in clusion, chiefly slight displacement of cell organelles.…”

Section: Resultssupporting

confidence: 58%

Demonstration by Electron Microscopy of Viruses in Cells Found by Light Microscopy to Contain Inclusion Bodies

Rossi¹,

Pauli²,

Luginbühl³

et al. 1972

Pathobiology

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Recently, a method was developed in this laboratory by means of which inclusion bodies found in cells during examination of histological sections might be processed for electron microscopy and by which the presence or absence of viruses in these bodies would be determined. The results of applying this method to the inclusion bodies of psittacosis, canine infectious hepatitis, equine viral rhinopneumonitis, bovine rabies and canine distemper are described. These demonstrate that definitive diagnosis can be established by this method.

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A Comparative Electron Microscopic Study on the Morphogenesis of Canine Distemper and Rinderpest Viruses

Cited by 12 publications

References 24 publications

Canine Distemper Virus

Canine Distemper Virus

Sporadic bovine meningo-encephalitis-isolation of a paramyxovirus

Demonstration by Electron Microscopy of Viruses in Cells Found by Light Microscopy to Contain Inclusion Bodies

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