A comparative burn wound model in the New Yorkshire pig for the histopathological evaluation of local therapeutic regimens: silver sulfadiazine cream as a standard

Hoekstra, M.J.; Hupkens, Pieter; Dutrieux, Richard P.; Bosch, M.M.C.; Brans, Toon; Kreis, R.W.

doi:10.1016/0007-1226(93)90111-n

Cited by 70 publications

(41 citation statements)

References 17 publications

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“…The animals were fed with a balanced mixture R 233 in the full physical and mental comfort eliminating the stress reactions. The skin burn wounds were inflicted according to Hoekstra's model [14]. In the study, three 16-week sows weighing about 35-40 kg were used.…”

Section: Methodsmentioning

confidence: 99%

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Application of Numerical Analysis of the Shape of Electron Paramagnetic Resonance Spectra for Determination of the Number of Different Groups of Radicals in the Burn Wounds

Olczyk

Komosińska-Vassev

Ramos

et al. 2017

Oxidative Medicine and Cellular Longevity

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Background. The evidence exists that radicals are crucial agents necessary for the wound regeneration helping to enhance the repair process. Materials and methods. The lineshape of the electron paramagnetic resonance (EPR) spectra of the burn wounds measured with the low microwave power (2.2 mW) was numerically analyzed. The experimental spectra were fitted by the sum of two and three lines. Results. The number of the lines in the EPR spectrum corresponded to the number of different groups of radicals in the natural samples after thermal treatment. The component lines were described by Gaussian and Lorentzian functions. The spectra of the burn wounds were superposition of three lines different in shape and in linewidths. The best fitting was obtained for the sum of broad Gaussian, broad Lorentzian, and narrow Lorentzian lines. Dipolar interactions between the unpaired electrons widened the broad Gaussian and broad Lorentzian lines. Radicals with the narrow Lorentzian lines existed mainly in the tested samples. Conclusions. The spectral shape analysis may be proposed as a useful method for determining the number of different groups of radicals in the burn wounds.

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Section: Methodsmentioning

confidence: 99%

“…The abovementioned procedure allowed a simultaneous degreasing of the samples. The tissue homogenates were dried to constant mass and weighed again [14]. …”

Section: Methodsmentioning

confidence: 99%

Application of Numerical Analysis of the Shape of Electron Paramagnetic Resonance Spectra for Determination of the Number of Different Groups of Radicals in the Burn Wounds

Olczyk

Komosińska-Vassev

Ramos

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…Four domestic four-month-old, pigs were used for the evaluation of wound repair because of many similarities between pig and human skin. Seventy-two contact burn wounds were inflicted on the right and left flanks of the pigs' body, according to the methods of Hoekstra et al [29] and Brans et al [30]. Pigs were housed according to the Good Laboratory Practice (GLP) Standards of Polish Veterinary Law.…”

Section: Methodsmentioning

confidence: 99%

Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

Olczyk

Komosińska-Vassev

Wisowski

et al. 2014

BioMed Research International

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The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process.

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“…In another study using rabbits, Pistacia lentiscus fatty oil (PLFO), and honey mixture was suggested to enhance healing of burn wounds as evidenced by faster contraction rates, especially when the mixture was applied during the inflammatory phase (26). It is worth mentioning that a standard burn wound model was developed in pigs to enable evaluation of histopathological parameters of wound healing under different circumstances (17) Sidr honey was proven to produce superior wound healing effects to thyme and spring honeys in thermally-and chemically-induced burn wounds in rabbits (19). In this study, the effects of Sidr honey, thyme, and spring honey were compared to commercially available and synthetic burn healing agents such as Mebo and Fusidin and the results were comparable.…”

Section: Laboratory Animals (Rats Mice and Rabbits)mentioning

confidence: 99%

Review of animal models used to study effects of bee products on wound healing: findings and applications

Hananeh

Ismail

Alshehabat

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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Non-healing wounds are associated with high morbidity and might greatly impact a patient's well-being and economic status. For many years, scientific research has focused on developing and testing several natural and synthetic materials that enhance the rate of wound healing or eliminate healing complications. Honey has been used for thousands of years as a traditional remedy for many ailments. Recently, honey has reemerged as a promising wound care product especially for infected wounds and for wounds in diabetic patients. In addition to its proposed potent broad-spectrum antibacterial properties, honey has been claimed to promote wound healing by reducing wound hyperaemia, oedema, and exudate, and by stimulating angiogenesis, granulation tissue formation and epithelialisation. Several animal models, including large animals, dogs and cats, and different species of laboratory animals have been used to investigate the efficacy and safety of various natural and synthetic agents for wound healing enhancement. Interpreting the results obtained by these studies is, however, rather difficult and usually hampered by many limiting factors including great variation in types and origins of honey, the type of animal species used as models, the type of wounds, the number of animals, the number and type of controls, and variation in treatment protocols. In this article, we provide a comprehensive review of the most recent findings and applications of published experimental and clinical trials using honey as an agent for wound healing enhancement in different animal models.

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A comparative burn wound model in the New Yorkshire pig for the histopathological evaluation of local therapeutic regimens: silver sulfadiazine cream as a standard

Cited by 70 publications

References 17 publications

Application of Numerical Analysis of the Shape of Electron Paramagnetic Resonance Spectra for Determination of the Number of Different Groups of Radicals in the Burn Wounds

Application of Numerical Analysis of the Shape of Electron Paramagnetic Resonance Spectra for Determination of the Number of Different Groups of Radicals in the Burn Wounds

Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

Review of animal models used to study effects of bee products on wound healing: findings and applications

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