A Comparative Analysis of Somatolactin-Related Immunoreactivity in the Pituitaries of Four Neopterygian Fishes and One Chondrostean Fish: An Immunohistochemical Study

Dores, Robert M.; Hoffman, Nicole E.; Chilcutt-Ruth, Tina; Lancha, Ana; Brown, Christopher L.; Marra, Luciano E.; Youson, John H.

doi:10.1006/gcen.1996.0049

Cited by 16 publications

(10 citation statements)

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“…The presence of ir-SL cells has been studied in a great number of fish. Nevertheless, this hormone has not been localized by IHC in Gymothorax meleagris (Dores et al 1996), though we believe that this gene is present in the pituitaries of all species of fish. The IHC is limited, in some cases, by the distinct specificity and sensitivity of the antibodies used.…”

Section: Discussionmentioning

confidence: 77%

“…In two species of holostean, Lepisosteus osseus and Amia calva, the SL and the melanocite stimulating hormone (MSH) are co-expressed in distinct degrees in the PI cells (Dores et al 1996). Thus the analyses of the evolution of the family of GH/PRL/SL genes have shown the presence of different protein variants of a single hormone.…”

Section: Discussionmentioning

confidence: 98%

“…Up to the present, the location of SL-producing cells in different species of fish has been performed mainly by immunohistochemistry (IHC; Rand-Weaver et al 1991b;Kaneko et al 1993a;Olivereau and Rand-Weaver 1994a,b;Zhu and Thomas 1995;Dores et al 1996;García-Hernández et al 1996;Vissio et al 1996Vissio et al , 1997Rendón et al 1997;Villaplana et al 1997;Johnson et al 1997;Saga et al 1999;Mousa and Mousa 1999;Salam et al 2000). The immunoreactive SL (ir-SL) cells were located principally in the PI of the hypophysis, bordering the neurohypophysial tissue.…”

Section: Discussionmentioning

confidence: 98%

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Co-expression of somatolactin mRNAs in the pituitary gland of gilthead sea bream

Herrero-Turrión

Velasco

Rodrı́guez

et al. 2002

Anatomy and Embryology

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The expression of mRNAs of the two types of somatolactin which have been found, up to the present, in the pituitary of gilthead sea bream (Sparus aurata) have been analyzed by in situ hybridization (ISH). The method of non-radioactive ISH, which we optimize in this study, uses oligonucleotides labelled in the 5' end with biotin or dioxygenin as probes. This allows simple and double ISH of high specificity and sensitivity to be performed. The distinct somatolactin oligoprobes used present a strong signal fundamentally in the pars intermedia of the pituitary gland. For the first time we present cells that co-express both the forms of mRNA mentioned above. Moreover, after studying three groups of different sizes, produced by asynchronic growth of the gilthead sea bream in industrial cultivation, we did not find qualitative differences in the levels of expression of the somatolactin gene.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Co-expression of somatolactin mRNAs in the pituitary gland of gilthead sea bream

Herrero-Turrión

Velasco

Rodrı́guez

et al. 2002

Anatomy and Embryology

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“…GH and PRL are present throughout the vertebrates (Kawauchi and Yasuda, 1989a), while SL is unique in teleosts (RandWeaver and Kawauchi, 1993;Dores et al, 1996). In teleosts, GH is involved in somatic growth (Donaldson et al, 1979), PRL in freshwater adaptation (Hirano, 1986) and SL in osmoregulation as well as final maturation (Rand-Weaver et al, 1992;Rand-Weaver and Swanson, 1993;Olivereau and RandWeaver, 1994a;Kakizawa et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Immunocytochemical Analysis of Pituitary Cells in Pre-spawning Chum Salmon

et al. 2000

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ABSTRACT-Pituitary hormones regulate various physiological functions during spawning migration in salmonid. Cytological features of pituitary cells were therefore immunocytochemically examined by use of antisera against homologous hormones in pre-spawning chum salmon (Oncorhynchus keta) caught from Ishikari Bay and the Chitose River in October, 1996. Immunoreactivity and sizes of pituitary cells were determined by a computer-aided image analyzing technique. Immunoreactivity in growth hormone (GH) cells was stronger with enlarged cell sizes in freshwater (FW) fish than in seawater (SW) ones of both sexes. Majority of prolactin (PRL) cells also had significantly stronger immunoreactivity with enlarged cell sizes in FW fish than in SW animals of both sexes. Immunoreactivity in somatolactin (SL) cells was markedly stronger with enlarged cell sizes in FW fish than in SW ones of both males and females. In addition, greater portions of SL cells were strongly stained in FW animals than in SW ones. A greater portion of gonadotropin (GTH) I cells had stronger immunoreactivity with reduced cell sizes in FW fish of both sexes, when compared with SW fish. Conversely, GTH II cells had significantly stronger immunoreactivity with enlarged cell sizes in FW ones of both sexes. In proopiomelanocortin (POMC)-derived hormone producing cells, adrenocorticotropin (ACTH) cells had stronger immunoreactivity in FW animals of both sexes, while cell sizes did not change. In melanotropes, the cells immunoreactive to α-melanophore stimulating hormone (α-MSH) antiserum had stronger immunoreactivity with reduced cell sizes only in FW males, while the cells immunoreactive to β-endorphin antiserum had stronger immunoreactivity with reduced cell sizes in FW fish than in SW animals of both sexes. Implication of these results was discussed along with previous reports on gene expression of pituitary hormone precursors.

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“…Immunocytochemical studies indicate that SLimmunoreactive cells are located in the pars intermedia of teleostean and holostean fish , Parhar & Iwata 1994, Dores et al 1996. These cells are periodic acid-Schiff positive in most species, but chromophobic in salmonids in which cloning and sequencing of cDNA SL shows a lack of Nglycosylation sites in the mature protein (Takayama et al 1991b).…”

Section: Introductionmentioning

confidence: 99%

Recombinant somatolactin as a stable and bioactive protein in a cell culture bioassay: development and validation of a sensitive and reproducible radioimmunoassay

Calduch-Giner¹,

Pendón²,

Mm³

et al. 1998

Journal of Endocrinology

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A recombinant somatolactin (SL) obtained by cloning and expression of sole SL cDNA was analyzed and used to develop a sensitive and specific RIA. In contrast to native proteins, which tend to dimerize and aggregate immediately after pituitary isolation, the majority of recombinant sole SL (rsSL) remained as a monomeric protein after long-term storage, as shown by size exclusion chromatography and Western blot. Using rsSL as a tracer and standard in the RIA, the minimum detectable dose and the midrange (ED 50 ) of the assay were 0·15 and 1·8-2·1 ng/ml respectively. Intra-and interassay coefficients of variation were 4·3% and 6·5% at ED 50 levels. Recombinant gilthead sea bream GH and recombinant trout GH did not show cross-reactivity, whereas a good parallelism between rsSL standard and serial dilutions of plasma and sole pituitary extracts was observed. In order to demonstrate some biological activity of rsSL, the ability of this recombinant product to prime gilthead sea bream phagocytes for in vitro enhancement of mitochondrial activity was examined by a chromogenic assay. A bell-shape dose-response curve was obtained with a maximum at 50 nM (1·2 µg/ml), similar to that reported previously for GH. Therefore, taking together all these data, it appears conclusive that rsSL is a long-term stable protein which retains, at least in part, biological activity, providing a useful tool to clarify the physiological role of fish SL.

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A Comparative Analysis of Somatolactin-Related Immunoreactivity in the Pituitaries of Four Neopterygian Fishes and One Chondrostean Fish: An Immunohistochemical Study

Cited by 16 publications

References 0 publications

Co-expression of somatolactin mRNAs in the pituitary gland of gilthead sea bream

Co-expression of somatolactin mRNAs in the pituitary gland of gilthead sea bream

Immunocytochemical Analysis of Pituitary Cells in Pre-spawning Chum Salmon

Recombinant somatolactin as a stable and bioactive protein in a cell culture bioassay: development and validation of a sensitive and reproducible radioimmunoassay

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