A common uptake system for serotonin and dopamine in human platelets.

Omenn, Gilbert S.; Smith, Lynne T.

doi:10.1172/jci109121

Cited by 47 publications

(17 citation statements)

References 31 publications

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“…In the present system, the K m for 5-hydroxytryptamine uptake was 1.6-2.2 μιηοΐ/ΐ (1.8 ± 0.25 μιηοΐ/ΐ, mean ± S. D., n = 4) with a F max of 16.5 pmol/5 min per 10 7 platelets. These results are slightly higher than those reported previously (11,16). This may be due to differences in the anticoagulant used, the extraction procedure for radioactive 5-hydroxytryptamine, or the dilution medium used for the platelet-rich plasma.…”

Section: -Hydroxytryptamine Carcinogenic Heterocyclic Aminescontrasting

confidence: 69%

Carcinogenic Tryptophan Pyrolysis Products Potent Inhibitors of Type A Monoamine Oxidase and the Platelet Response to 5-Hydroxytryptamine

Manabe¹,

Kanai²,

Ishikawa³

et al. 1988

Clinical Chemistry and Laboratory Medicine

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The effects of carcinogenic heterocyclic amines and -carbolines on 5-hydroxytryptamine-induced human platelet aggregation,-on the uptake of 5-hydroxytryptamine by platelets, and on human monoamine oxidase activity were investigated. Of the dietary carcinogens and -carbolines studied, carcinogenic tryptophan pyrolysis products had greater pharmacological activities than other heterocyclic amines. The carcinogenic tryptophan pyrolysis products, 3-amino-l,4-dimethyl-5H-pyrido[4,3-b]indole and 3-amino-l-methyl-5H-pyrido[4,3-b]indole, which have been identified in the dialysis fluid of uraemic patients, were the most potent inhibitors of the aggregation response to 5-hydroxytryptamine, with IC 50 (the concentrations causing 50% inhibition) values of 10 μιηοΐ/ΐ and 50 μιηοΐ/ΐ, respectively. 3-Amino-l,4-dimethyl-5H-pyrido[4,3-b]indoleand 3-amino-l-methyl-5H-pyrido[4,3-b]indole by themselves did not induce platelet aggregation, although these dietary carcinogens structurally resemble 54iydroxytryptamine. Kinetic analyses showed that 3-amino-l,4-dimethyl-5H-pyrido [4,3^b]indole and 3*amino-l-methyl-5H-pyrido[4,3-b]indole were potent competitive inhibitors of 5-hydroxytryptamine uptake by platelets with /ζ 18 μπιοΐ/ΐ and 42 μιηοΐ/ΐ, respectively. Furthermore, carcinogenic tryptophan pyrolysates as well as -carbolines were found to be competitive selective inhibitors of monoamine oxidase 'type A'. IntroductionAmong such carcinogens, 3-amino-l ,4-dimethyl-5H-During the last decade, a new series of heterocyclic pyrido[4,3-b]indole, 3-amino-l-methyl-5H-pyramines has been isolated as potent mutagens from ido[4,3-b]indole and γ-carboline derivatives, strongly pyrolysates of amino acids and proteins and from inhibit human platelet aggregation by inhibiting prosbroiled fish and meat (1, 2). All the mutagenic het-taglandin endoperoxide synthetase (7). In the course erocyclic amines so far tested have been shown to be of the previous study, we found that 3-amino-l,4-carcinogenic in experimental animals (1,2). Recently, dimethyl-5H-pyrido[4,3-b]indole and 3-amino-lsome mutagenic and carcinogenic heterocyclic amines methyl-5H-pyrido [4,3-b]indole are also potent inhibsuch as 3,amino-l,4-dimethyl- 5H-pyrido[4,3-b]indole, itors of 5-hydroxytryptamine-induced human platelet 3-amino-l-methyl, 5H-pyrido[4,3-b]indole, 2-amino-aggregation. Since aggregation induced by 5-hydroxyo-methyldipyridoIl^-arS'^'-dlimidazole and 2-ami-tryptamine is mediated by a specific receptor (8,9), nodipyrido[l>a:^2'-d]imidazote were identified in il is perhaps relevant that the tryptophan pyrolysis the dialysis fluid of patients with uraemia, and in products, 3-amino-l,4-dimethyl-5H-pyrido[4,3-b]-human plasma (3-6).indole and 3-amino-l -methyl-5H-pyrido[4,3-b]indole, are structurally related to 5-hydroxytryptamine (ser-

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Section: -Hydroxytryptamine Carcinogenic Heterocyclic Aminescontrasting

confidence: 69%

Carcinogenic Tryptophan Pyrolysis Products Potent Inhibitors of Type A Monoamine Oxidase and the Platelet Response to 5-Hydroxytryptamine

Manabe¹,

Kanai²,

Ishikawa³

et al. 1988

Clinical Chemistry and Laboratory Medicine

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“…The higher percentage overlap between brain and platelets could be due to the many shared functions and proteins that are found in both tissues. Omenn and colleagues [24] could show, e.g., that serotonin can be removed by reuptake into serotonergic neurons as well as into platelets. In addition, the relatively small size of the platelet proteome could lead to a higher overlap compared to the plasma.…”

Section: Data Mining and Comparison With Literaturementioning

confidence: 99%

HUPO Brain Proteome Project: Summary of the pilot phase and introduction of a comprehensive data reprocessing strategy

Hamacher¹,

Apweiler²,

Arnold³

et al. 2006

Proteomics

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The Human Proteome Organisation (HUPO) initiated several projects focusing on the proteome analysis of distinct human organs. The Brain Proteome Project (BPP) is the initiative dedicated to the brain, its development and correlated diseases. Two pilot studies have been performed aiming at the comparison of techniques, laboratories and approaches. With the help of the results gained, objective data submission, storage and reprocessing workflow have been established. The biological relevance of the data will be drawn from the inter-laboratory comparisons as well as from the re-calculation of all data sets submitted by the different groups. In the following, results of the single groups as well as the centralised reprocessing effort will be summarised and compared, showing the added value of this concerted work.

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“…However the lower level of platelet catecholamines and the high level of platelet serotonin (Ferrari, et al, 1985), measured simultaneously in the same children, suggest that the elevated plasmatic concentration of norepinephrine and epinephrine might be, at least partly, due to their less efficient storage in platelets. In fact, in thrombocytes several biochemical mechanisms are common for catecholamines and serotonin" Dopamine and serotonin uptakes depend on the same membrane carrier (Omenn & Smith, 1978) and accordingly platelet uptakes of serotonin (Launay et al, submitted) and dopamine (Boullin & O'Brien, 1972) are "normal" in autistic children. Epinephrine, norepinephrine, dopamine, and serotonin are costored in the same "dense granules" (Da Prada, Richards, & Kettler 1981) which possess a "maximal packet size" (Costa et al, 1978).…”

Section: Discussionmentioning

confidence: 99%

Catecholamines metabolism in infantile autism: A controlled study of 22 autistic children

Launay

Bursztejn

Ferrari

et al. 1987

J Autism Dev Disord

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In a group of 22 autistic children aged 5 to 16 years and a group of normal controls matched for age and sex, catecholamines metabolism was investigated in plasma, platelets, and urine. This investigation was part of a research project in which several biological parameters (including serotonin) were explored simultaneously in the same children. In the autistic group, epinephrine and norepinephrine were significantly elevated in plasma, while epinephrin, norepinephrine, and dopamine were significantly lower in isolated platelets. No significant difference was found between the two groups for the urinary excretion of epinephrine, norepinephrine, dopamine, DOPAC, and MHPG. Other differences between the two groups in the statistical correlations of several biochemical parameters also suggest abnormalities of bioamine metabolism in the platelets of autistic children.

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A common uptake system for serotonin and dopamine in human platelets.

Cited by 47 publications

References 31 publications

Carcinogenic Tryptophan Pyrolysis Products Potent Inhibitors of Type A Monoamine Oxidase and the Platelet Response to 5-Hydroxytryptamine

Carcinogenic Tryptophan Pyrolysis Products Potent Inhibitors of Type A Monoamine Oxidase and the Platelet Response to 5-Hydroxytryptamine

HUPO Brain Proteome Project: Summary of the pilot phase and introduction of a comprehensive data reprocessing strategy

Catecholamines metabolism in infantile autism: A controlled study of 22 autistic children

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